SU1109990A1 - METHOD OF CULTIVATION OF THE VIRUS OF THE VASCINE - Google Patents

METHOD OF CULTIVATION OF THE VIRUS OF THE VASCINE

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Publication number
SU1109990A1
SU1109990A1 SU3552029/15A SU3552029A SU1109990A1 SU 1109990 A1 SU1109990 A1 SU 1109990A1 SU 3552029/15 A SU3552029/15 A SU 3552029/15A SU 3552029 A SU3552029 A SU 3552029A SU 1109990 A1 SU1109990 A1 SU 1109990A1
Authority
SU
USSR - Soviet Union
Prior art keywords
virus
cells
mat
vascine
cultivation
Prior art date
Application number
SU3552029/15A
Other languages
Russian (ru)
Inventor
Б.Я. Бодин
Ю.А. Черняев
В.М. Гневашев
Original Assignee
Всесоюзный научно-исследовательский ящурный институт
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Всесоюзный научно-исследовательский ящурный институт filed Critical Всесоюзный научно-исследовательский ящурный институт
Priority to SU3552029/15A priority Critical patent/SU1109990A1/en
Application granted granted Critical
Publication of SU1109990A1 publication Critical patent/SU1109990A1/en

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Abstract

Способ выращивания вируса ящура, включающий заражение матровым вирусом суспензионной культуры клеток ВНК-21 и последующую инкубацию в ней вируса, отличающийся тем, что, с целью уменьшения расхода количества матрового вируса, перед заражением клетки культуры концентрируют, заражение концентрата клеток матровым вирусом осуществляют в дозе 1 - 2 LDна клетку, перед инкубацией проводят адсорбцию вируса в течение 50 - 70 мин при комнатной температуре и разведение вируссодержащей суспензии питательной средой до исходной концентрации.The method of growing FMD virus, which includes infecting with Math virus a suspension culture of BHK-21 cells and subsequent incubation of the virus in it, characterized in that, in order to reduce the consumption of the amount of mat virus, before infection the culture cells are concentrated, infection of the cell concentrate with a mat virus is performed in dose 1 - 2 LD cells, prior to incubation, the virus is adsorbed for 50-70 minutes at room temperature and diluted with the virus-containing suspension in a nutrient medium to the initial concentration.

SU3552029/15A 1983-02-09 1983-02-09 METHOD OF CULTIVATION OF THE VIRUS OF THE VASCINE SU1109990A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU3552029/15A SU1109990A1 (en) 1983-02-09 1983-02-09 METHOD OF CULTIVATION OF THE VIRUS OF THE VASCINE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU3552029/15A SU1109990A1 (en) 1983-02-09 1983-02-09 METHOD OF CULTIVATION OF THE VIRUS OF THE VASCINE

Publications (1)

Publication Number Publication Date
SU1109990A1 true SU1109990A1 (en) 2000-03-27

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ID=60517205

Family Applications (1)

Application Number Title Priority Date Filing Date
SU3552029/15A SU1109990A1 (en) 1983-02-09 1983-02-09 METHOD OF CULTIVATION OF THE VIRUS OF THE VASCINE

Country Status (1)

Country Link
SU (1) SU1109990A1 (en)

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