SU1089118A1 - Strain bacillus subtilis 65 - Google Patents

Abstract

STAMM BACILLUS SUBTILIS 65 (collection of the Central Museum of Industrial Microorganisms of the Institute of Scientific Research Institute of Genetics, collection number CMPM B-238 7) - PRODUCER about AMYLASES.

Description

About 90. JI

The invention relates to the production of enzymes and is a new strain of BaciJJus subtij 65 used for the biosynthesis of OC-amylase. Many microorganisms are known as producers of oi, -amylases. The enzyme of the amylolytic complex produced by bacteria of the genus Bacillus has industrial value. Thus, the strain BaciUus su tilis SK 52 is known - producer of ob-amyl. The producer's activity in production conditions is on average 190 units / ml AU, in flasks 26 units / ml AU 1. The closest to the proposed is the strain and producer oL -Amylase Bacillus subtilis RA - possesses active activity in the fermenter 401.2 units / ml AC 2. The disadvantages of the BaciJIus subtilis SK-52 and Bacilfus subtiE RA strains are colonial-morphological variability. Both strains isolate a colonial-morphological variant of M, which does not synthesize ob-amylase at all. The strain of Bacillus subtifis RA is characterized by a particularly high morphological variability, as a result of which the strains show a decrease in amylotic activity and degeneration of the strains, besides the fluctuation of the amylolytic activity of certain individuals in the population. The variability coefficient of the BaciJJus subtifis SK-52 strain is 38.5%, the BacilZus subtiZis HA strain is 41.0%, while the Bacillus subtilis-65 strain has a variability coefficient of 16%, which indicates the genetic stability of the strain. The purpose of the invention is to obtain a strain with high activity under deep cultivation conditions. Culture BaciEIus subtilis-65 obtained from strain You. subtiJis SK-52 as a result of stepwise selection under the action of 250 μg / ml, N-methyl-N-nitpo-N-nitrosome in the phosphate buffer pH 5.0. The Bacillus subtiHs-65 strain is stored in the All-Union Collection of the Institute of All-Russian Scientific Research Institute of Genetics under the number CM CM PM-2387 and is characterized by 82 following morphological, cultural and physiological prizes. Morphological signs. Bacilfus subtifis-65 culture is a bacillus (1.7–3.0) x (4.0–0.0) microns grown on a rich medium and has the appearance of thick, large sticks, sometimes arranged in chains. The rods are motile with the help of pereitrihialn flagella, gram-positive. The culture forms Cylindrical or ellipsoid spores, (1.0-1.5) x (0.6-0.9) microns in size. Cultural and physiological. the signs. After 24 h of incubation in a thermostat at 37 ° C, the culture forms flat, dry, dense colonies with a characteristic white granular coating, which can be easily removed by a loop of agar. Diameter - 2-5 mm. The edges are almost flat or slightly cut. Other types of colonies are not marked. On a slice of potatoes, growth is plentiful in the form of a grayish-white dry edgy of morsh film. On the surface of the meso-peptone broth, Ultra 65 forms a thin film of an elder-white color, and the broth is transparent. The strain BacifJus subtiZis 65 systems aerob with an optimal temperature of growth of 37 ° C at a pH of 6.2-7.0. Ultra absorbs glucose, sugar, fructose, arabinose hydrates starch, pentonizes oloko, decompresses gelatin, nitrates sets to nitrites, on the red. Clarke forms acetylmethylarbonyl. Example. For the cultivation of B. subti Cis 65 producer of o-amyase, the production ratio of medium composition is used, g / l: Potato starch239 Corn extract 12.43 Brewer's yeast2.19 Lactose 2.87 Copper sulfate 0.0036 Ammonium phosphate dibasic 8.4 Sodium 20.28 sulphate0, 14 3 Potassium sulphate2, 87 Calcium chloride0, 45 Sterile-cooked and inoculated with you strain, -lus subtifis 65 is incubated in a thermostat at 37 ° C on a rocking chair at 180 rpm, as well as in semi-production conditions in fermentors with a capacity of 3 m 48 h growth in culture but the liquid is determined by amylase acti NOSTA BER method. The control strains are the production 8h strain BaciFfus subtifis SK-52 and VacilJus subtifis RA. The test results are shown in the table. As can be seen from the table you. subtilis 65 is characterized by amnpolitical activity. Amshtolitichesky activity in semi-production conditions compared with the strain you. subtifiis SK-52 increased by 18.3 and by 15% compared with Bacillus subtiEis RA. The expected annual effect from the introduction of Bacillus subtilis 65 is approximately 30 rubles.

Bacillus subtilis SK-52

In flasks of Bacillus subtilis RA BacilJus subtifis 65

Bacillus subtilis SK-52

In the fermenter Bacillus subtiiis RA Bacillus subtilis 65

ten

348.0 375.5 553.5

3033.2

ten

3700

5562.5

Claims (1)

STRAIN BACILLUS SUBTILIS 65 (collection of the Central Museum of Industrial Microorganisms of the Institute VNIIgenetics, collection number CMPM V-2387) - PRODUCER oL ~ AMILASE. producer flasks