SU1066551A1 - Method of diagnosis of activity of lung tuberculosis - Google Patents

Abstract

METHOD OF DIAGNOSTIC ACTIVITY OF PULMONARY TUBERCULOSIS by cytochemical blood analysis, characterized in that, in order to improve the accuracy of the method, the percentage of positive reactions to the Meptxi monocyte esterase enzyme is determined at 72 hours after administration of tuberculin, and with increasing levels of positively reactive monocytes, the monocyte enzyme is 72 hours after the introduction of tuberculin. active pulmonary tuberculosis.

Description

about o: o: eat

The invention relates to medicine and can be used to decide on the activity of pulmonary tuberculosis and the effectiveness of treatment.

A known method for diagnosing pulmonary tuberculosis activity is by comparing serum protein fractions before and after subcutaneous administration of tuberculin. The assessment is made on the basis of changes in the correlation of albumin and globulins, as well as on the local, general and focal reaction of the organism to tuberculin 1.

This test has the following disadvantages: changes in serum protein fractions can be not only during this process, but also in other infectious, inflammatory diseases, and can also be caused by allergization of the organism.

There is also known a method for diagnosing leukemic tuberculosis activity by T-and-B-rosettoobrazuyu lymphocytes of peripheral blood of patients with pulmonary tuberculosis. The method is based on the fact that the peripheral blood leukocytes defend, centrifuged, washed with medium No. 199, the precipitate is suspended. The sheep erythrocytes are added to the suspension. Leukocytes with erythrophytes are incubated, centrifuged, kept in the refrigerator. Subsequently, the sockets are fixed with aldehyde, the supernatant is sucked off, the precipitate is pipetted and a drop of suspension is placed on a glass slide. Smears are dried, fixed, colored with azure-P-eosin. For a rosette-forming cell, a lymphocyte is taken, fixing 3 or more red blood cells on its surface 2.

However, this method does not have sufficient accuracy, is difficult to perform, and requires a lot of labor / cost, therefore it is difficult to use in everyday phthisiology practice.

The purpose of the invention is to improve the accuracy of the method.

The goal is achieved according to the method of diagnosing the activity of tubercle. pulmonary tuberculosis.

The method is carried out as follows: OOM.

Blood samples are taken from the patient's finger, air dried blood smears are tricked in formalin vapors for 5–10 minutes at room temperature and washed in water for 20 minutes. G (incubation time 24 hrs. The incubation mixture consists of two solutions: hexazotene pararosaniline and sodium nitrite solution. Equal. We pacTiiopoB, shake for a few seconds, the liquid will not turn straw-yellow in color .- After incubation, the swabs are washed in distilled water, dried and stained with 2 ° / oh methyl green. In smears, 100 mo1; ocytes are counted and the number of positive and negative monocytes is determined before and 72 hours after the subcutaneous administration of turberculin (in percent). About the presence of this enzyme and indicates a red-brown diffuse monocyte staining.

With an increase in the percentage of positively reagent monocytes from 88 to 100%, active pulmonary tuberculosis is diagnosed.

Example 1. Patient P., 49 years old, was admitted to the therapeutic department to clarify the activity of tuberculous changes in the lungs. Complaints of insignificant weakness, cough with scanty sputum, loss of appetite. With percussion and auscultation local signs of tuberculosis are absent. Mycobacterium tuberculosis was not detected in the sputum. Radiographically: in the upper parts of both lungs, - in the II, single small-medium foci with Fuzzy contours. Blood test: leukocytes 4,500, eosinophils 2, bacillus 2, segmental-type 54, lymphocytes 29, monocytes 13, ESR 12 mm / h, erythrocytes 4.750.000, Hb 7 & units

Cytochemical index of monocytes 48 hours after administration of tuberculin 88%. Diagnose active pulmonary tuberculosis. Further observation of the patient confirmed the conclusion. The indicator of positively reusing monocytes is 98.0%, negative "zero monocytes - 2.0 ° / o.

Example 2. Patient L. S.37 years old was admitted to a tuberculosis dispensary to clarify the activity of tuberculosis changes in the upper lobe of the right lung. The patient has mild signs of tuberculosis intoxication, with percussion and auscultation, local signs of tuberculosis are not detected, mycobacterium tuberculosis is not detected in the sputum; Radiographically: in the upper lobe of the right lung, single medium sized lesions with fuzzy contours. Blood test: leukocytes 5.800, zoosinophils 3, bacillus 3, segmented bacteria 70, lymphocytes 17. monocytes 7, ESR 10 mm / h, erythrocytes 4.300.000, PV 74 units

Cytochemical indicator of monocytes on the enzyme exterase 100%. The diagnosis of active pulmonary tuberculosis. Further observation confirmed the correctness of the diagnosis.

Thus, the method permits a more accurate diagnosis of the active anti-tuberculosis, 1066551.

34

easy treatment of the lungs, timely execution, available, does not require curative treatment, and assess the effectiveness of the equipment and the large laboriousness of chemotherapy treatment costs, it can be used on a daily basis. The proposed method is simple in technical phthisiological practice.

Claims (1)

METHOD FOR DIAGNOSTIC OF LUNG TUBERCULOSIS ACTIVITY by cytochemical blood test, characterized in that, in order to increase the accuracy of the method, the percentage of monocytes positively reacting to the enzyme esterase is determined 48 hours after administration of tuberculin, and with an increase in the content of positively reacting monocytes from 88 to 100% diagnosed with active pulmonary tuberculosis. 1066551 '