SU1064954A1 - Method of extracorporal detoxication of organism - Google Patents

Abstract

METHOD OF EXTRACORPORAL DETOXICATION OF THE ORGANISM by using a suspension of isolated liver cells separated from blood by a semipermeable membrane, characterized in that, with the purpose of reusing liver cells, a suspension of isolated liver cells in a nutrient medium is placed in closed shells of a semipermeable membrane of 1.01, 2 cm, the formed granules are introduced into a column filled with a blood substitute, pro. bleed through it, at the end of the perfusion granules are washed and placed in a nutrient medium for storage.

Description

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4 The invention relates to medicine, in particular to hepatology and toxicology, and can be used in the treatment of diseases and toxic damages of the liver in order to collect its lost functions. There is a method of extracorporeal detoxification of the organism by using a suspension of isolated cells of the cell, separated from the blood by the semi-proton cell membrane 1. However, the well-known method can not always and not everywhere be used because of its complexity, the obligatory presence of complex equipment, i.e. centers There are annairati artificial kidney, peritoneal dialysis. In addition, it is not always possible to perform an intervention on the abdominal cavity (this is peritoneal dialysis), since there is an adhesive process in the abdominal cavity, which limits the possibility of performing this operation. Therefore, peritoneal dialysis has its limitations and cannot always be used to treat patients with acute liver failure. When using these methods, the cell suspension of the allogeneic liver is applied only once and then ejected. Therefore, the method requires a large amount of preparation of biological material and stock of cell suspension. The purpose of the invention is the reuse of liver cells. This goal is achieved by using an extracorporeal detoxication of the body by using a suspension of isolated liver cells separated from blood by a semipermeable membrane, a suspension of isolated liver cells and nutrient medium are placed in closed shells of a semipermeable membrane of 1.0-1.2 cm, formed the granules are introduced into a column filled with a blood substitute, blood is passed through it, at the end of the perfusion the granules are washed H are placed in a nutrient medium for storage. The method is carried out as follows. A suspension of isolated liver cells (obtained by the enzymatic-mechanical method) was diluted in medium 199 to a concentration of 210 (20 ml / ml), filled with them. Accurate graduation and a system of flexible silicon of horses. At the same time, the preparation of a semi-permeable cellophane film is carried out, cutting it into squares of a certain shape, which are initially placed in a physiological solution, and then transferred into a solution of dioecide, 1: 2000, followed by distilled water. A section of the film is put on the end of the silicone hose and, retreating 1 cm from its tip, ligates the ligature, after which 1 m of cell suspension is introduced into this cavity and, tightening the lining, the silicone hose is removed. Excess film and material seam is removed. 250-300 granules are prepared and placed in a vial with medium 199. When taking the required amount of granules, they fill the column through which blood is perfused. For this purpose, vessels (artery and vein) are picked up and catheterized to form an arteriovenous shunt. The pellet column is connected to the shunt in this way. In order for arterial blood to enter the column from the bottom of the top, fill it in all over the tank and out through the upper cannula, which is connected to the venous system. Perfusion is continued for 2 hours at a rate of 100-120 ml / min. The column is then disconnected from the shunt and the blood returns to the system. After this, the column and pellets are washed and the latter are placed for 1-2 days on Wednesday 199. With this, P) we create all the conditions for the restoration of their reserves and functions, as they are in a culture medium where all the necessary metabolites are present. for their restoration and maintenance of viability. Consequently, the cell. ki can restore their metabolic balance and can be reused to detoxify the blood. In addition, cells i found in granules (they have been subjected to treatment with toxic substances) can increase their detoxification properties, as if they were training - working out their detoxification function. Metabolizing many substances, synthesizing many enzymes and metabolites, the cell seems to be close in these conditions to the object of action, i.e., biologic IMC media, and will have a much greater effect on the restoration of the equilibrium of the biological environment, as it is in small volumes . Therefore, the processes of diffusion and osmosis, ultrafiltration will be carried out between the granules and the medium significantly faster than the B system of the artificial kidney apparatus. After the cells are kept in medium 199, they are again placed in the column, the latter is connected to the arteriovenous shunt and perfused for 1.5-2.0 hours. Then the column and the granules are washed with Hens solution and the latter are placed on medium 199. After 1 2 days Granules are used again for detoxification of the body. Thus, the wounds are used 3-5 times to detoxify the blood and the body according to the indicated method. The column was connected with granules in 3 patients with 6 poisoned Cltt and hepato-renal insufficiency. An arteriovenous shunt is connected to a 350 ml KortoN, which is filled with rales and blood substitute so that the arterial KjWBb is filled from the bottom. Blood outflow

regulate the level of lift of the clone (40-60 cm) and the speed of blood flow. The perfusion is carried out within 1.5-2.0 hours. During the operation, clinical observation is carried out in patients and after 30-60 minutes blood is drawn: 5

for biochemical research. As a result of the operation, it was established that the patient's general condition (consciousness, BP, P1, respiratory rate) improves, patients become contact, answer questions. In the biochemical 10 control, the concentration of toxic substances is reduced by. 50-60% (ammonia, bilirubin) and. The concentration of some metabolites increases by 20% -40% (urea, cholesterol). All this . allows to make a conclusion about the favorable course of research institutes of clinical and laboratory tests of control

patients and the favorable effect of the ongoing. operations that have a significant effect on the outcome of the disease.

The method will find a wide application in the department; In addition to intensive therapy for the treatment of acute liver failure in liver damage, its use provides multiple effective use of suspension of isolated liver cells, affecting the degree of degradation of the isolated cells. The procession of the granules allows the cells to be successfully separated from the blood and to prevent the preventive reactions and at the same time bring them closer to the object of action - biological fluids.

Claims (1)

METHOD FOR EXTRACORPORAL ORGANISM DETOXICATION by using a suspension of isolated liver cells separated from the blood by a semipermeable membrane, characterized in that, for the purpose of reusing liver cells, a suspension of isolated liver cells in a nutrient medium is placed in closed shells of a semi-permeable membrane with a volume of 1, ΟΙ, 2 cm ^ formed granules are introduced into a column filled with a blood substitute, blood is passed through it, at the end of perfusion, the granules are washed and placed in a nutrient medium for storage. <10645