SU1049542A1 - Process for preparing suspension of spores clostridium tyrobutyricum - Google Patents

Description

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o 1-: otku 1; ute PROMR IKI dispute dopil.

The greatest bdizkigl i and zoobrotemits gyu tsnileoka ooshnotm and positivol-th effect of the cripples criOooG:; from-gssh; ous;: that; zi1g g; ps: p Wed. tyrobu - ryrioMni, r; genus om:; trg | waggon: jaoc :: Tj ii itat.p ;; u; about Wednesday to Enkubirová -1is ioode, g, USMA kudtur: l image: for ;; and. task copy c :: op.j.

SG; S5SOBO, M PRODUCT% SG: RO 0 l yii-l T r. P LOS OS incubated not only in the middle of the environment and in a supportive dispute and long-term Pul yriipoiian-te.

However: pveech; -; ::; e, the way they work out PG) and to e ;: i ::: i i et alal :: i i o o b c r y y o; i dsol.zd-i: unified n, -gv ;; kov, and Tajf; ke boJd-, -consumption and time. .-: e Tip /: - ;, phycicome ;;; ani-1 escoo d himchchss; Сее юадейсал-и е и а disputes :::: Odessa oh by: r.geg: silt by this method / ynca .vi; v : c-; aT to complete iia-kx properties per hour l-; :: oti ka 1x: rmoreziste 1T yuot7 ..

The invention's invention is to shorten ;; and the potential of the process and its determination:;

Another goal is achieved: a .EHO method for obtaining a suspension cncip CCostr.i.diura tyrobptyricuiu: 1 eduo:.: Atrio 11o: the topic of sowing in a nutrient diet, e, tsu and 1; The quality of the dispute, the incubation of the follow-on culture of the wasp; culture of Syesa; sterile skimmed milk and honey ratio of 99: 1 to, the constants of value, pH ere, poy 5, I - 5, 2

Necessity of co-cultivation of MG1s of noxious bacteria eme 3 of g htmr :: milk, about 3 times with third g; tokhokkami mozhet be explained by handing over education.

Known; that the process of intense growth that is carried out in the art of mass bacteria can be hindered by the sudden creation of environment 1: eb; convenient conditions for their life / id-guest; and against the background of the rapid development of vegetational 1 (le: -current, and the nature of these conditions is hardening; features of the type of clotridia used. When working with C., tyrobiityricnm 1-1, the induction of spores, the development is usually significant; This seems to be caused by some kind of spedif | -h-s; - mi; s; with them they bring about the vital activity of the microorganisms of this nida.Therefore, the usual (such KatK sudden depletion of the medium; d, the sharp dehydration of the cells, the effect air, etc.) does not have the desired effect, and the dispute titer of the CP. tyrobu-Lyj-i.curn iip; -i et does not exceed 10 m.

In the case of joint development of bacteria and bacteria and mesophilic m-one: Isd: Streptococcus s, Modoc with mey: - In bacteria, only crushed bacteria develop in bacteria. C2. tyrobuty-XlcuT, cannot develop in modoco, - also, it is not capable of fermenting MODO-1NY sugar. As cTpeirroKOKicoB develops, a part of the lactose sugar peroxides with them into milk acid, which, neutralized by honey, is an energy substrate for doostridium, With the appearance of; The salt of lactic acid begins to live with the bacteria and bacteria. Over time; and the c-pede will accumulate a sufficient amount of their vegetative ktetok. However, as the p-oste mesophidic MODEs of Streptococcus pH, the pH of the medium gradually decreases to a value of 5.1-5.2, which is 1: the lower limit of the pM values, at which: - where the bacteria are found in the species CC. tyrobutyricum. Upon reaching this value ;;; l; active kiodotnooty and abruptly come adverse conditions for the further development of cudtum 1: lostridium, which is responsible for the intensification of sporulation.

Thus, with the help of mesophilic lactic acid cells, the conditions are created for the accumulation in the medium of sufficiently large numbers of vegetative cells and the interventions of the latter as a result of lowering the pH to a level that prevents them from using energetic material accumulated in the medium.

For the implementation of the method it is possible to use any mesophilic lactic streptococci.

S ,, Eactis (strains 438

551sr

eight

6

551

And) 5

899

619157, 162,

five

eight

-7

5 187 and 213} f S. diaceti actis (51, 222, 405, 506, 598, 752, 81C, 851, 923 and 522), S.cremori (strains fg, 3M-S, 49 and 613). The strains were selected proizvopno but from the collection of the Altai branch of VNIIM :: and they all gave roughly the same yield of disputes CP. tyrobutyricum (IIJTHMMH G, G, K-13) - about 10 spores / ml. Example. 3 10 ml of sterile skimmed milk with chalk introduces one bacteriological loop freshly from the culture of any strain of lactic acid streptococcus belonging to the species S.Eactis, S.cremoris or S. diacetlBactis, which do not show an inhibitory effect on CP.tyrobricum, and 0, 1 ml of an 18-hour broth culture of C.C. tyrobutyricum. Oily acid bacteria are introduced as compactly as possible to the bottom of the tube. Sowing incubated at 5 days. After this time the clot contains at least 10 spores in 1 ml. The resulting suspension of C. tyrobutyricum spores and lactic acid CTpenTOKOKicoB is placed in a refrigerator where it can be stored without changing the spore titer for up to six months or more. Before use, any dilution of the resulting mixture, which is three ph r-ph

- the number of cells SgeagoNe „

- the number of protective cells, c.tyr pulyfcum

- amount of dispute C. tyroduti / cum

 It is used for hot water work, heated in a water bath at 80-85 seconds for 15 minutes, which is necessary for the thermal activation of spore germination and sufficient for complete release of the lactic acid streptococcus cells that have disappeared from it. processing. The illustrations of the co-culture and culture used are shown graphically in FIGS. 1-3. The resulting spores can be applied in any kind of research. For example, 0.1–0.2 ml of the first dilution of the mixture is taken on a petri dish with a continuous lawn for inoculation of butyric acid bacteria, and five times the dilution of spore suspension is used to simulate the number of spore anaerobes in ordinary raw milk. The proposed method is simple to implement, does not require additional equipment and is time consuming and completely eliminates the mechanical and chemical effects on the spores in the production process. In addition, the duration of the process is reduced and its cost decreases.

Claims (1)

.54 57 METHOD FOR GETTING SUSPENSION · ' CLOSTRIDIUM TYR0BUTYRI CUM DISPUTE, providing for inoculation into the nutrient medium and incubation of the test culture to form a predetermined number of spores, characterized in that, in order to reduce the duration of the process and reduce its cost, the test culture is incubated together with a culture of Streptococcus mesophilic lactic streptococcus species Streptococcus E diacefiEactis and Streptococcus cremoris, which do not inhibit Clostridium tyrobutyricum, on a nutrient solution consisting of sterile skim milk and chalk ratio of 99: 1. until a pH of 5.1-5.2 is reached.