SK5868Y1 - Antiseptic formulation based on organic peroxides - Google Patents

Abstract

An antiseptic formulation for disinfection of spaces and surfaces based on organic peroxides as the principal active component in combination with an organic solvent as carrier and secondary active component, wherein the content of the organic peroxides being of from 1 to 3 % by weight and the content of the organic solvents being of from 97 to 99 % by weight.

Description

Technical field

The utility model relates to a disinfectant based on organic peroxides in organic solvents and belongs to the field of disinfectants for disinfecting the working and public environment, i. j. spaces and surfaces.

BACKGROUND OF THE INVENTION

Disinfection is an act applied for centuries using chlorine lime and chloramine. Disinfectants are now applied to disinfect surfaces, tools, laundry, medical supplies, drinking and process water (pool chemistry). The development of new disinfectants is under pressure from increasing criteria for the effectiveness of disinfectants, given the wide range of bacterial, fungal, viral and other human infections as well as the quality and safety of the use of disinfectants. The volume of use of disinfectants is considerable and can be roughly divided into surface disinfection (hospitals, workplaces with infectious material), water disinfection (sewage treatment plants, water treatment plants), air disinfection and disinfection of objects (medical material and the like).

Disinfection means a set of measures for the destruction of micro-organisms by means of physical, chemical and combined processes. Sterilization is a set of measures that kill all microorganisms on objects, including highly resistant spores, microscopic fungi, protozoa developmental stages, and possibly helminths. Sterilization techniques must be so effective that no more than one non-sterile per million sterilized articles is involved.

The effectiveness of disinfectants can be low (leading to the destruction and death of microorganisms) and static (temporarily preventing the growth of microorganisms, or leading to a decrease in growth activity).

The object of the antimicrobial effect is viruses, protozoa, bacteria, actinomycetes, fungi, protozoa, algae and higher small organisms, as well as subcellular structures such as primes and toxic proteins.

The most common disinfection mechanism is:

• oxidation (chlorine, H ₂ O ₂ , ozone, peroxo compounds), • hydrolysis (acids, alkalis, hot water), • formation of salts with proteins (heavy metal salts, halogenation), • coagulation of proteins in the cell (quaternary ammonium salts - KAS , metals, phenol, alcohols), changes in cell membrane permeability (KAS, aldehydes), penetration into the enzymatic system (metals, formaldehyde, phenol).

Disinfectant requirements:

• wide spectrum of efficacy or high selective efficacy • action at low concentrations • action at short exposures • effect does not affect the presence of proteins • without unpleasant odor • does not leave toxic residues • simple dosing • biodegradable • stable • well • suitable packaging.

General principles for disinfection of objects:

• In the case of objects contaminated with biological material, first disinfection, followed by cleaning. • Alternation of disinfectants to prevent possible selection or resistance of microbes to long-term use of the product. • Monitor indoor air quality, do not exceed applicable hygiene regulations.

Overview of microbiological active substances:

• hydroxides and other alkalis, • acids and some of their salts, • oxidizing substances (ozone, hydrogen peroxide, metal peroxides, persulphates, peroxoacetic acid, peroxo borate), mechanism of action - strong oxidative oxygen damage at birth;

• halogens (chlorine, hypochlorites, chloramines, iodine, iodophores), mechanism of action - oxidative damage due to oxygen and halogen at birth, reaction of iodine to thiol groups of proteins, • alcohols and ethers (methanol, ethanol, propanol, isopropanol , benzyl alcohol glycerol, 1,2-propanediol, ether, ethylene oxide) • aldehydes (formaldehyde, glutaraldehyde, glyoxal), mechanism of action - high reactivity of aldehyde group, ability to react with functional groups of proteins, alkylation reactions with nucleic acid bases • phenol derivatives (phenol, alkylphenols, cresols, diphenyls, 2-hydroxybiphenyl, chlorophenols, quinolines), mechanism of action - ability to dissolve cell wall lipoids • guanidines (chlorhexidine, polyhexamethylenebiguanidine hydrochloride) • quaternary ammonium salts (benzalkonium chloride, cellular mechanism of action) due to increased permeability

Table 1 presents the spectrum of antimicrobial activity of selected agents:

Table 1

Disinfectant Vegetative forms of bacteria spores viruses Mycobacterium thuberculosis Phenol and its derivatives + - + + Chlorine and chlorine preparations + - + + aldehydes + + + + KAS + - + - Organic peroxyacids + + + + Iodine and its derivatives + + + +

Development trends in disinfection:

• Combined preparations with a broad spectrum of activity. • Peroxy compounds II. generation, Further development of chlorine preparations, • “Renaissance” of quaternary ammonium salts, • New iodophor preparations, • Preparations with a higher degree of disinfection.

There is a large amount of disinfectants on the world, European and Slovak markets, whether for personal hygiene, surface sterilization, water sterilization, or for other application purposes. The individual formulations utilize the active ingredients described in detail above.

Preparations for surface disinfection of hands and skin; Ajatin, Betadine Liquid Soap, Biotenside, Cutasept F, Cutasept G, Deladerm, Dodesept, Chloramine B, Chlorhexidine Solution 20%, Jodisol, Jodonal B, Manoprotito, Manox, Manusept, Octeniderm, Octeniserm, Persteril, Pliva Sept - Tincture, Primas , Sagrosept, Septonex, Septonex tincture, Spitaderm, Sterilium, Triformin, Triformin HR.

Disinfectants in dentistry: Deconex Dental BB, Dezipur, Neodisher Septo 2000, Neodisher Septo 2000, Orotol Ultra, Persteril, Sekudrill, Sekusept Forte, Sekusept Pulver.

Disinfectants for surface sterilization of instruments: Aldesol, Cidex, Desam GK, Desident - solution, Desident spray 94, Desoform, Dezipur, Dikonite, Gali Valerio Solutio decemplex Medicamenta, Gigasept FF, Glutaraldehyde, Hibicet, Chloramine B, Chlorhexidine Solution, Kohrsolin ID, Lysetol AF, Lysetol FF, Lysoformin 3000, Neodisher Septo 2000, Neodisher Septo 2000 neu, Neodisher Septo DN, Neodisher Septo SF, Perfectan, Persteril, Spit Sept Tincture, Presept, Sekudrill, Sekusept Extra N, Sekusept Forte, Sekusept Plus, Sekusept Pulver and Virkon.

Disinfectants for laundry sterilization: Demyro D, Dikonit, Chloramine B, Chloramine BM, Chloramine BS, Chloramine Extrasept, Orthosan BF 12, Persteril, Savo, Savo Direct (Savo Direct with fragrance), Stamid.

Disinfectants for surface sterilization: Dosilin S, Dusept, FD 312, FD 322, FD 350, Forten, Foxamln, Gastri-sil, Gastril, Guttar, Chloramine B, Javel Solid, Jodonal A, Chloramine BM, Chloramine BS, Chloramine Extrasept, Chlorodetal, Chlorine Lime, Incidin Extra, Incidin Plus, Incidur (Incidur SP), Incidur Spray, Jodonal B, Lysoformin 3000, Microbac Forte, Microside Liquid, Neodisher Alka 300, Neodisher Dekonta, Neoform D, Neoquat S, Neoquat S, Neoseptal CL , Neoseptal IM, Novrokept, Orthosan BF 12, Perform, Persteril, Spit Sept tincture, Presept, Puron DA, Puron KD, Savo, Savo Direct against mildew, Savo Direct (Savo Direct with fragrance), Septonex, Septonex tincture , Stamid, Ventarsan, Virkon, Weicosil, Weigosept DF Spray, Weiquat LF.

SK 5868 Υ1

Sterilization methods:

Physical sterilization (wet heat / PS /, dry heat / HS /, radiation, plasma)

Chemical sterilization (formaldehyde, ethylene oxide)

Formaldehyde sterilization - Formaldehyde evaporation from formalin, vacuum up to -90 kPa, temperature up to 80 ° C, exposure determined for each device separately

Sterilization with ethylene oxide - effects under vacuum and overpressure, temperatures significantly below 100 ° C, proper ventilation after sterilization is necessary.

After evaluating all the above-mentioned disinfectants and sterilizers, it can be stated that:

• practically all of these formulations use as a basic, carrier component, one of the highly reactive sterilizing agents, whether aldehyde-based, oxygen-releasing or halogen-based, at birth; • some formulations exhibit corrosive properties to common materials (metal , paper, wood, plastic) or loss of color and colored surface coatings, • most of the formulations for topical application are built in the form of concentrates for dilution to working concentrations for manual topical application by washing / washing, • only part of the formulations are adapted for spray application • In the profile of the disinfectants offered, there are practically absent or only on the rise products for disinfecting the working environment, as categorizing disinfectants for the sterilization of premises and especially for the sterilization of surfaces are categorized.

In the context of EU legislation, a new segment has been created for disinfection, namely disinfection of jobs in connection with cyclicality of work changes in production facilities and for disinfection of public areas (public transport, gastronomic facilities, museums, libraries, waiting etc.) in connection with epidemics. and pandemics of infectious diseases and the protection of the public health of individual clients. For this segment it was necessary to develop a new type of products, namely the formulation of disinfectants, namely non-toxic, material intact aerosols of applicable disinfectants.

Efforts to apply a 'mist' of chlorinated water formulations, such as Aquasystem, are evident from literature and practice. A disadvantage of these formulations and applications is the presence of toxic chlorinated (halogen) reactive forms of HCl, HCl, NaClO, ClO ₂ , and the like.

As regards the hydrogen peroxide formulation, patents of hydrogen peroxide solutions in water are known and documented, with the addition of:

• Peroxic acids, such as peracetic acid (Persteril ™), • Aromatic acids, • Aromatic alcohols, • Quaternary ammonium salts, • Arylsulfonic acid surfactants, • Betaines, alkyl betaines, • Fromaldehyde, which is now discriminated against as a proven carcinogen • Nonionic emulsifiers (eg polysorbate 80), essential oils (silica silica, oil Valencia oil), (for example, patents EP 1562430, WO 2005110090, WO 0035289, WO2004045281, WO2007038265, EP1452484, WO2006016145 and many others). Formulations of disinfectants with organic peroxides as the main active ingredient are not known.

The essence of the technical solution

SUMMARY OF THE INVENTION The object of the present invention was to provide a disinfectant for disinfecting the working and public environment, i. j. areas and surfaces free of toxic substances and with rapid fading effect, short exposure, inertia to hard and soft surfaces, self-degradation, higher efficiency. This task was accomplished by formulating a disinfectant for disinfecting the work and public environment, i. j. Peroxide-based spaces and surfaces as the main active ingredient in combination with an organic solvent as a carrier and a co-active ingredient, characterized in that the peroxides are organic peroxides, the organic peroxide content is from 1 to 3% by weight and the organic solvent content is 99%. up to 97% by weight.

The organic peroxides may be selected from tert-butyl peroxide (TBP), benzoyl peroxide (BP), tert-butyl hydroperoxide (TBHP).

The organic solvent may be ethanol and / or 1-propanol and / or 2-propanol and / or ether.

Suitably, the disinfectant composition comprises stabilizers that extend the shelf life of the composition. It has been tested that without stabilizers, the stability of the formulation is about 6 months.

SK 5868 Υ1

It is preferred that the disinfectant is capable of being placed in an aerosol applicator. The disinfectant composition of the present invention has a high volutahility and thus dispersibility of the composition, has a short but intense effect, surface inertness and rapid elimination from the application areas, i. j. according to this invention the prepared disinfectants show experimentally proven all the required properties: fast smoke effect, short exposure, inertness to hard and soft surfaces, self-degradation.

The organic peroxides tertbutylperoxide (TBP) and tertbutyl hydroperoxide (TBHP) show demonstrably higher antimicrobial activity. This activity was tested by the OECD prescribed method by determining the minimum inhibitory concentration (MIC) parameter on microplates on prescribed microbial strains of Escherichia coli, CCM 3954, as representative of G ⁺ bacteria, Staphylococcus aureus CCM 2022, as representative of G ⁺ bacteria and potentially yeast mycotic strain. in the form of Candida albicans, CCM 2123. A higher MIC value presents a higher disinfectant efficiency. The results of a comparative study of hydrogen peroxide and two selected organic peroxides are presented in the following table. 2

Table no. 2

agent Escherichia coli CCM 3954 Staphylococcus aureus CCM 2022 [MIC] Candida albicans CCM 2123 Hydrogen peroxide 32 32 8 TBP 64 128 16 TBHP 1012 1012 64

The results show at least 8-fold higher antimicrobial activity and thus antiseptic or disinfectant effectiveness. The latter higher efficiency is at least of the order of magnitude higher since it is potentiated by the second property specifically required for the disinfectant effect on the room, namely volatility, respectively volutability, determined by lower boiling point of the substance and unlimited miscibility with volatile organic solvents.

Table no. 3

agent Boiling point of pure substance [° C] Miscible with water Miscible with organic solvents Hydrogen peroxide 108 without limit limited TBP 109 limited without limit TBHP 80 without limit without limit

According to this technical solution, three sub-formulations of the disinfectant can be prepared according to the intended use:

1. Soft preparation for general non-specific disinfection of areas with a disinfectant effect on a common workplace microflora intended for disinfection of normal workplaces where no work with significant pathogens resulting from the nature of the work is carried out. This product should be used in households, public areas and normal work environments. The specificity of this type of preparation was the declaration of zero environmental impact and virtually zero cumulative corrosivity to all common materials. This formulation has been evaluated for efficacy on conventional test strains prescribed for aseptic and disinfectants.

2. Preparation of medium for specific disinfection of areas with disinfectant effect on common and specially formulated microflora of specialized workplaces intended for disinfection of specific workplaces where specific pathogens are suspected or the possibility of infection of a larger group of population (hospitals, public transport, canteens) , cosmetics, hairdressing, workplaces and so on) resulting from the nature of the work. The specificity of this kind of preparation will be declared higher specific efficacy against prescribed model pathogens.

3. A strong preparation for the specific disinfection of areas with disinfectant effect on conventional and specially formulated microflora of specialized workplaces intended for disinfection of specific workplaces where work is carried out with significant pathogens or suspected of being infected by a larger population (hospitals, research workplaces, workplaces) working with veterinary material and the like) resulting from the nature of the work. The specificity of this type of preparation will be declared specific activity against selected specific pathogens (extended range of tested microbial

Strains such as nosocomial infections of Staphylcoccus aureus, Pseudomonas aeruginosa, Candida albicans and the like).

An example of the antimicrobial (poor, disinfectant) efficacy of the product collection according to this technical solution on three selected model microorganisms, in competition with the most widely used disinfectants on the market, is given in the following table. 4

Table no. 4

agent Escherichia coli CCM 3954 Staphylococcus aureus CCM 2022 [MIC] Candida albicans CCM 2123 formulation according to the invention - soft 64 64 8 preparation according to the invention - medium 128 128 8 preparation according to the invention 1012 1012 Germ-X 2 1 0 Quartz - forte 256 512 0 Dettol 64 128 0 Fresh S 8 4 4

Examples of technical solution

Example 1

Disinfectants are prepared according to the following table no. 5 without water addition (v, v):

Table no. 5

P. č. agent Content of main active ingredient I. [% v, v] Content of minor active ingredient I. [% v, v] Content of minor active ingredient II. [% v, v] Content of minor active ingredient III. [% v, v] 1 soft TBHP 1% ethanol 99% - - 2 medium TBHP 2% ethanol 98% - - 3 TBHP 3% ethanol 97% - - 4 TBP 1% ethanol 99% - - 5 medium TBP 2% ethanol 98% - - 6 TBP 3% ethanol 97% - -

The formulations are placed in an aerosol applicator and are ready for use.

Example 2

Disinfectants are prepared according to the following table no. 6 + water ad 100% (v, v):

SK 5868 Υ1

Table no. 6

P. č. agent Content of main active ingredient I. [% v, v] Content of minor active ingredient I. [% v, v] Content of minor active ingredient II. í% v, v] Content of minor active ingredient III. [% v, v] 1 soft TBHP 1% 2-propanol 99% - - 2 medium TBHP 2% 2-propanol 98% - - 3 strong TBHP 3% 2-propanol 97% - - 4 soft TBP 1% 2-propanol 99% - - medium TBP 2% 2-propanol 98% - - 6 strong TBP 3% 2-propanol 97% - -

The formulations are placed in an aerosol applicator and are ready for use.

Industrial usability

Disinfectants according to this technical solution have wide application in the sphere of medical, food, hygiene and also in the area of low consumption in households. They can be used e.g. for the disinfection of public transport, public catering facilities, public workplaces, public areas, medical facilities and instruments, etc.

Claims (5)

PROTECTION REQUIREMENTS 1. Disinfectant for disinfecting the working and public environment, i. j. Peroxide-based spaces and surfaces as the main active ingredient in combination with an organic solvent as a carrier and a co-active ingredient, characterized in that the peroxides are organic peroxides, the organic peroxide content is from 1 to 3% by weight and the organic solvent content is from 97 to 99% by weight. Disinfectant composition according to claim 1, characterized in that the organic peroxides are selected from the group of tert-butyl peroxide (TBP), benzolyperoxide (BP), tert-butyl hydroperoxide (TBHP). Disinfectant composition according to claim 1 and 2, characterized by the solvent being ethanol and / or 1-propanol and / or 2-propanol and / or ether. Disinfectant composition according to claims 1 and 2, characterized by a source. Disinfectant composition according to claims 1 to 3, characterized by an aerosol applicator.