Lek, Tovarna Farmacevtskih In Kemicnih Izdelkov, D.D.
Zavod Republike Slovenije Za Transfuzijo Krvi
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Application filed by Lek, Tovarna Farmacevtskih In Kemicnih Izdelkov, D.D., Zavod Republike Slovenije Za Transfuzijo KrvifiledCriticalLek, Tovarna Farmacevtskih In Kemicnih Izdelkov, D.D.
Priority to SI9600206ApriorityCriticalpatent/SI9600206B/en
Priority to DE69626829Tprioritypatent/DE69626829T2/en
Priority to EP96926042Aprioritypatent/EP0846169B1/en
Priority to AT96926042Tprioritypatent/ATE234918T1/en
Priority to PCT/SI1996/000018prioritypatent/WO1997006251A1/en
Priority to AU66345/96Aprioritypatent/AU703091B2/en
Publication of SI9600206ApublicationCriticalpatent/SI9600206A/en
Publication of SI9600206BpublicationCriticalpatent/SI9600206B/en
The submitted invention belongs to the area of pharmaceutical industry and refers to new monoclonic antibodies and their fragments that bind to an accessible surface of human recombinant TNF-Alpha, TNF-Alpha analogues and soluble TNF-Alpha p55 and p75 receptors. The invention also refers to stable cellular lines of hybridomas that are able to produce such monoclonic antibodies, the procedure for their preparation and to pharmaceutical preparations that contain them. Further on, the invention refers to the method for a quantitative determination of human TNF-Alpha, free and complexed with soluble TNF-Alpha p55 and p75 receptors and the kit that is used for this. The monoclonic antibodies known so far and the reagents developed from them could not be used for a simultaneous determination of the free TNF-Alpha and their soluble p55 and p75 receptors as well as their complexes. The invention also refers to TNF-1 analogue to TNF His107/108, Cys95/148 that is used as an antigen for the immunization of mice in the complex with TNF-Alpha receptors.
Claims (4)
Λ PATENTNI ZAHTEVKI 1. Monoklonska protitelesa in njihovi fragmenti, ki so zmožni vezati se ali na dostopno površino humanega TNF-α ali TNF-α analogov ali prostih topnih TNF-α receptorjev p55 ali p75 ter njihovih kompleksov, označeni s tem, da jih pridobimo z gojenjem stabilne celične linije hibridomov ECACC 95101016. | Z. Stabilna celična linija hibridomov ECACC 95101016.1. Monoclonal antibodies and fragments thereof capable of binding either to the accessible surface of human TNF-α or TNF-α analogues or free soluble TNF-α receptors p55 or p75 and their complexes, characterized in that they are obtained by growing a stable cell line of ECACC 95101016 hybridomas Z. Stable cell line of hybridomas ECACC 95101016.3.Uporaba monoklonskih protiteles in njihovih fragmentov po zahtevku 1 za pripravo testnega kita za določanje humanega TNF-α, prostega in kompleksiranega s topnima TNF-α receptorjema p55 in p75, v bioloških vzorcih. 4 Uporaba monoklonskih Drotiteles in njihovih fragmentom ki so zmožni vezati se ali na dostopno površino humanega TNF-α ali TNF-α analogov ali prostih topnih TNF-α receptorjev p55 ali p75 ter njihovih kompleksov, označeni s tem, da jih pridobimo z gojenjem stabilne celične linije hibridomov ECACC 95071217, za pripravo testnega kita za določanje humanega topnega TNF-α receptorja p55, prostega ali kompleksiranega s TNF-α, v bioloških vzorcih. ζ .Uporaba monoklonskih protiteles in njihovih fragmentov, ki so zmožni vezati se ali na dostopno površino humanega TNF-α ali TNF-α analogov ali prostih topnih TNF-α receptorjev p55 ali p75 ter njihovih kompleksov, označeni s tem, da jih pridobimo z gojenjem stabilne celične linije hibridomov ECACC 95071218, za pripravo testnega kita za določanje humanega topnega TNF-α receptorja p75, prostega ali kompleksiranega, v bioloških vzorcih.Use of monoclonal antibodies and fragments thereof according to claim 1 for the preparation of a test kit for the determination of human TNF-α, free and complexed with soluble TNF-α receptors p55 and p75, in biological samples. Use of monoclonal Drotiteles and their fragments capable of binding either to the access surface of human TNF-α or TNF-α analogues or free soluble TNF-α receptors p55 or p75 and their complexes, characterized in that they are obtained by culturing a stable cell hybridoma line ECACC 95071217, for the preparation of a test kit for the determination of human soluble TNF-α receptor p55, free or complexed with TNF-α, in biological samples. U .Use of monoclonal antibodies and fragments thereof capable of binding either to the access surface of human TNF-α or TNF-α analogues or free soluble TNF-α receptors p55 or p75 and their complexes, characterized by being obtained by culturing stable cell lines of hybrids ECACC 95071218, for the preparation of a test kit for the determination of human soluble TNF-α receptor p75, free or complex, in biological samples.6 .Metoda za kvantitativno določanje humanega TNF-α, prostega ali kompleksiranega s topnima TNF-α receptorjema p55 ali p75, v bioloških vzorcih z uporabo monoklonskih protiteles ali njihovih fragmentov po zahtevku 1.A method for the quantitative determination of human TNF-α, free or complexed with soluble TNF-α receptors p55 or p75, in biological samples using monoclonal antibodies or fragments thereof according to claim 1.7 .Kit za določanje humanega TNF-α, prostega ali kompleksiranega s topnima TNF-α receptorjema p55 in/ali p75, v bioloških vzorcih, ozhačen s tem, da vsebuje monoklonska protitelesa in njihove fragmente po zahtevku 1.Kit for the determination of human TNF-α, free or complexed with soluble TNF-α receptors p55 and / or p75, in biological samples, characterized in that it contains monoclonal antibodies and fragments thereof according to claim 1.//
SI9600206A1995-08-041996-06-28Monoclonic antibodies versus soluble tnf-alpha p55 and p75 receptors and tnf-alpha and their ana
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