SG11202112409WA - Method - Google Patents

Method

Info

Publication number
SG11202112409WA
SG11202112409WA SG11202112409WA SG11202112409WA SG11202112409WA SG 11202112409W A SG11202112409W A SG 11202112409WA SG 11202112409W A SG11202112409W A SG 11202112409WA SG 11202112409W A SG11202112409W A SG 11202112409WA SG 11202112409W A SG11202112409W A SG 11202112409WA
Authority
SG
Singapore
Application number
SG11202112409WA
Inventor
Andrew John Heron
Mark John Bruce
Rebecca Victoria Bowen
Luke Alexander Mcneill
Simon Rafael Villarreal
Samuel John Martin
Rebecca Anne Stafford-Allen
Original Assignee
Oxford Nanopore Tech Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Tech Plc filed Critical Oxford Nanopore Tech Plc
Publication of SG11202112409WA publication Critical patent/SG11202112409WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
SG11202112409WA 2019-05-22 2020-05-22 Method SG11202112409WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1907244.6A GB201907244D0 (en) 2019-05-22 2019-05-22 Method
PCT/GB2020/051260 WO2020234612A1 (en) 2019-05-22 2020-05-22 Method

Publications (1)

Publication Number Publication Date
SG11202112409WA true SG11202112409WA (en) 2021-12-30

Family

ID=66867555

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202112409WA SG11202112409WA (en) 2019-05-22 2020-05-22 Method

Country Status (9)

Country Link
EP (1) EP3973076A1 (en)
JP (1) JP2022533243A (en)
KR (1) KR20220011665A (en)
CN (1) CN114269947A (en)
AU (1) AU2020280243A1 (en)
CA (1) CA3139866A1 (en)
GB (1) GB201907244D0 (en)
SG (1) SG11202112409WA (en)
WO (1) WO2020234612A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
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GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
CN113862264A (en) * 2021-12-03 2021-12-31 北京齐碳科技有限公司 Adapters, constructs, methods and uses for sequencing of target polynucleotides
CN114262735A (en) * 2021-12-15 2022-04-01 成都齐碳科技有限公司 Adaptors for characterising polynucleotides and uses thereof
GB202118906D0 (en) * 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
CN114457145B (en) * 2022-01-29 2023-08-11 成都齐碳科技有限公司 Linkers, constructs, methods and uses for characterizing target polynucleotide sequencing
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
WO2023223033A1 (en) 2022-05-18 2023-11-23 Oxford Nanopore Technologies Plc Compositions and methods for nucleic acid extraction and purification
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method

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US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
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AU2008217579A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
EP2158476B8 (en) 2007-05-08 2019-10-09 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
EP2195648B1 (en) 2007-09-12 2019-05-08 President and Fellows of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
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WO2010086603A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Enzyme mutant
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US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
CN103460040B (en) 2011-02-11 2016-08-17 牛津纳米孔技术有限公司 Saltant type hole
WO2012164270A1 (en) 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method
KR20140090633A (en) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 Method of characterizing a target polynucleotide using a pore and a hel308 helicase
GB201120910D0 (en) 2011-12-06 2012-01-18 Cambridge Entpr Ltd Nanopore functionality control
CN104126018B (en) 2011-12-29 2021-09-14 牛津纳米孔技术公司 Enzymatic process
CN104136631B (en) 2011-12-29 2017-03-01 牛津纳米孔技术公司 Method using XPD unwindase characterising polynucleotides
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
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CN105209634B (en) * 2013-03-08 2020-05-12 牛津纳米孔技术公司 Enzyme arrest method
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AU2015208919B9 (en) 2014-01-22 2021-04-01 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
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KR20170042794A (en) 2014-09-01 2017-04-19 브이아이비 브이지더블유 Mutant csgg pores
GB201609221D0 (en) * 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method

Also Published As

Publication number Publication date
WO2020234612A1 (en) 2020-11-26
GB201907244D0 (en) 2019-07-03
EP3973076A1 (en) 2022-03-30
JP2022533243A (en) 2022-07-21
CN114269947A (en) 2022-04-01
AU2020280243A1 (en) 2021-12-02
KR20220011665A (en) 2022-01-28
CA3139866A1 (en) 2020-11-26

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