SG11202111965YA - Auxotrophic selection methods - Google Patents

Auxotrophic selection methods

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Publication number
SG11202111965YA
SG11202111965YA SG11202111965YA SG11202111965YA SG11202111965YA SG 11202111965Y A SG11202111965Y A SG 11202111965YA SG 11202111965Y A SG11202111965Y A SG 11202111965YA SG 11202111965Y A SG11202111965Y A SG 11202111965YA SG 11202111965Y A SG11202111965Y A SG 11202111965YA
Authority
SG
Singapore
Prior art keywords
selection methods
auxotrophic selection
auxotrophic
methods
selection
Prior art date
Application number
SG11202111965YA
Inventor
James Patterson
Matthew Porteus
Volker Wiebking
Original Assignee
Auxolytic Ltd
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Auxolytic Ltd, Univ Leland Stanford Junior filed Critical Auxolytic Ltd
Publication of SG11202111965YA publication Critical patent/SG11202111965YA/en

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    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12Y201/00Transferases transferring one-carbon groups (2.1)
    • C12Y201/03Carboxy- and carbamoyltransferases (2.1.3)
    • C12Y201/03002Aspartate carbamoyltransferase (2.1.3.2)
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SG11202111965YA 2019-05-08 2020-05-08 Auxotrophic selection methods SG11202111965YA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962844930P 2019-05-08 2019-05-08
US201962904725P 2019-09-24 2019-09-24
PCT/US2020/032114 WO2020227637A1 (en) 2019-05-08 2020-05-08 Auxotrophic selection methods

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SG11202111965YA true SG11202111965YA (en) 2021-11-29

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US (1) US20220325301A1 (en)
EP (1) EP3966339A4 (en)
JP (1) JP2022532535A (en)
KR (1) KR20220018495A (en)
CN (1) CN114026243A (en)
AU (1) AU2020267598A1 (en)
BR (1) BR112021022110A2 (en)
CA (1) CA3138030A1 (en)
IL (1) IL287886A (en)
MA (1) MA55912A (en)
MX (1) MX2021013522A (en)
SG (1) SG11202111965YA (en)
WO (1) WO2020227637A1 (en)

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WO2022197693A2 (en) * 2021-03-15 2022-09-22 490 BioTech, Inc. System of stable gene expression in cell lines and methods of making and using the same
CN113215168B (en) * 2021-04-29 2023-04-28 华中科技大学同济医学院附属同济医院 Ovarian cancer cell drug resistance marker and application thereof

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US20030224411A1 (en) * 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US9181529B2 (en) * 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
EP2971027B1 (en) * 2013-03-15 2019-01-30 Amyris, Inc. Use of phosphoketolase and phosphotransacetylase for production of acetyl-coenzyme a derived compounds
CA2933134A1 (en) * 2013-12-13 2015-06-18 Cellectis Cas9 nuclease platform for microalgae genome engineering
WO2017053729A1 (en) * 2015-09-25 2017-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nuclease-mediated genome editing of primary cells and enrichment thereof
GB201618414D0 (en) * 2016-11-01 2016-12-14 Patterson James Regulated cell lines and methods of use thereof

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EP3966339A4 (en) 2023-01-25
BR112021022110A2 (en) 2022-01-04
WO2020227637A1 (en) 2020-11-12
KR20220018495A (en) 2022-02-15
MA55912A (en) 2022-03-16
MX2021013522A (en) 2021-12-10
AU2020267598A1 (en) 2021-12-16
US20220325301A1 (en) 2022-10-13
CN114026243A (en) 2022-02-08
JP2022532535A (en) 2022-07-15
CA3138030A1 (en) 2020-11-12
IL287886A (en) 2022-01-01
EP3966339A1 (en) 2022-03-16

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