SG11202111786RA - Codon optimized synthetic nucleotide sequences encoding cry2ai protein and uses thereof - Google Patents

Codon optimized synthetic nucleotide sequences encoding cry2ai protein and uses thereof

Info

Publication number
SG11202111786RA
SG11202111786RA SG11202111786RA SG11202111786RA SG11202111786RA SG 11202111786R A SG11202111786R A SG 11202111786RA SG 11202111786R A SG11202111786R A SG 11202111786RA SG 11202111786R A SG11202111786R A SG 11202111786RA SG 11202111786R A SG11202111786R A SG 11202111786RA
Authority
SG
Singapore
Prior art keywords
cry2ai
protein
nucleotide sequences
sequences encoding
codon optimized
Prior art date
Application number
SG11202111786RA
Inventor
Geetha Lakshmi Mangena
Dwarkesh Singh Parihar
Paresh Verma
Udayasuriyan V
Sudhakar D
balakrishnan N
Mohankumar S
Original Assignee
Dcm Shriram Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dcm Shriram Ltd filed Critical Dcm Shriram Ltd
Publication of SG11202111786RA publication Critical patent/SG11202111786RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11202111786RA 2019-04-24 2020-04-20 Codon optimized synthetic nucleotide sequences encoding cry2ai protein and uses thereof SG11202111786RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201911016327 2019-04-24
PCT/IN2020/050370 WO2020217252A1 (en) 2019-04-24 2020-04-20 Codon optimized synthetic nucleotide sequences encoding cry2ai protein and uses thereof

Publications (1)

Publication Number Publication Date
SG11202111786RA true SG11202111786RA (en) 2021-11-29

Family

ID=70617184

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202111786RA SG11202111786RA (en) 2019-04-24 2020-04-20 Codon optimized synthetic nucleotide sequences encoding cry2ai protein and uses thereof

Country Status (12)

Country Link
EP (1) EP3947698A1 (en)
JP (1) JP7458417B2 (en)
KR (1) KR20220012254A (en)
AR (1) AR118791A1 (en)
AU (1) AU2020260763A1 (en)
BR (1) BR112021021426A2 (en)
CA (1) CA3137811A1 (en)
IL (1) IL287436A (en)
MX (1) MX2021013026A (en)
SG (1) SG11202111786RA (en)
WO (1) WO2020217252A1 (en)
ZA (1) ZA202108265B (en)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5530195A (en) * 1994-06-10 1996-06-25 Ciba-Geigy Corporation Bacillus thuringiensis gene encoding a toxin active against insects
US6121521A (en) * 1998-04-01 2000-09-19 Novartis Ag Chimeric insecticidal protein and DNA coding therefor
CN100353846C (en) * 2000-08-25 2007-12-12 辛根塔参与股份公司 Novel insecticidal toxins derived from bacillus thuringiensis insecticidal crystal proteins
AU2002252974B2 (en) * 2001-01-09 2006-12-14 BASF Agricultural Solutions Seed US LLC Bacillus thuringiensis insecticidal proteins
AR035799A1 (en) * 2001-03-30 2004-07-14 Syngenta Participations Ag INSECTICIDE TOXINS ISOLATED FROM BACILLUS THURINGIENSIS AND ITS USES.
EP2822963A2 (en) * 2012-03-09 2015-01-14 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
MX2019004648A (en) * 2016-10-21 2019-05-23 Vestaron Corp Cleavable peptides and insecticidal and nematicidal proteins comprising same.

Also Published As

Publication number Publication date
CA3137811A1 (en) 2020-10-29
JP2022531146A (en) 2022-07-06
AU2020260763A1 (en) 2021-11-18
ZA202108265B (en) 2022-09-28
BR112021021426A2 (en) 2021-12-21
JP7458417B2 (en) 2024-03-29
IL287436A (en) 2021-12-01
KR20220012254A (en) 2022-02-03
MX2021013026A (en) 2022-02-10
WO2020217252A1 (en) 2020-10-29
CN114008208A (en) 2022-02-01
EP3947698A1 (en) 2022-02-09
AR118791A1 (en) 2021-11-03

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