SG11202111525XA - Stable targeted integration - Google Patents

Stable targeted integration

Info

Publication number
SG11202111525XA
SG11202111525XA SG11202111525XA SG11202111525XA SG11202111525XA SG 11202111525X A SG11202111525X A SG 11202111525XA SG 11202111525X A SG11202111525X A SG 11202111525XA SG 11202111525X A SG11202111525X A SG 11202111525XA SG 11202111525X A SG11202111525X A SG 11202111525XA
Authority
SG
Singapore
Prior art keywords
targeted integration
stable targeted
stable
integration
targeted
Prior art date
Application number
SG11202111525XA
Inventor
Scott Bahr
Michael Johns
James Ravellette
Joaquina Mascarenhas
Trissa Borgschulte
Original Assignee
Sigma Aldrich Co Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co Llc filed Critical Sigma Aldrich Co Llc
Publication of SG11202111525XA publication Critical patent/SG11202111525XA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
SG11202111525XA 2019-04-18 2020-04-20 Stable targeted integration SG11202111525XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962835810P 2019-04-18 2019-04-18
PCT/US2020/028991 WO2020215077A2 (en) 2019-04-18 2020-04-20 Stable targeted integration

Publications (1)

Publication Number Publication Date
SG11202111525XA true SG11202111525XA (en) 2021-11-29

Family

ID=71833405

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202111525XA SG11202111525XA (en) 2019-04-18 2020-04-20 Stable targeted integration

Country Status (8)

Country Link
US (1) US20220195465A1 (en)
EP (1) EP3956460A2 (en)
JP (1) JP7472167B2 (en)
KR (1) KR20220008274A (en)
CN (1) CN114026241A (en)
CA (1) CA3136659A1 (en)
SG (1) SG11202111525XA (en)
WO (1) WO2020215077A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022197749A1 (en) * 2021-03-15 2022-09-22 Donald Danforth Science Center Targeted insertion via transposition

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101999410B1 (en) 2011-04-05 2019-07-11 더 스크립스 리서치 인스티튜트 Chromosomal landing pads and related uses
WO2014205192A2 (en) 2013-06-19 2014-12-24 Sigma-Aldrich Co. Llc Targeted integration
US20210309988A1 (en) 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
EP3665291B1 (en) 2017-08-11 2021-10-06 Boehringer Ingelheim International GmbH Integration sites in cho cells

Also Published As

Publication number Publication date
WO2020215077A2 (en) 2020-10-22
JP7472167B2 (en) 2024-04-22
US20220195465A1 (en) 2022-06-23
CN114026241A (en) 2022-02-08
EP3956460A2 (en) 2022-02-23
WO2020215077A3 (en) 2020-12-03
CA3136659A1 (en) 2020-10-22
KR20220008274A (en) 2022-01-20
JP2022529063A (en) 2022-06-16

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