SG11202105625UA - Methods for generating and using organoids and tissue therein - Google Patents

Methods for generating and using organoids and tissue therein

Info

Publication number
SG11202105625UA
SG11202105625UA SG11202105625UA SG11202105625UA SG11202105625UA SG 11202105625U A SG11202105625U A SG 11202105625UA SG 11202105625U A SG11202105625U A SG 11202105625UA SG 11202105625U A SG11202105625U A SG 11202105625UA SG 11202105625U A SG11202105625U A SG 11202105625UA
Authority
SG
Singapore
Prior art keywords
organoids
tissue
generating
methods
Prior art date
Application number
SG11202105625UA
Inventor
Amanda Vegoe
Timothy D O'brien
Beth Lindborg
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of SG11202105625UA publication Critical patent/SG11202105625UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/19—Growth and differentiation factors [GDF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/905—Hyaluronic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
SG11202105625UA 2018-12-19 2019-12-18 Methods for generating and using organoids and tissue therein SG11202105625UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862781858P 2018-12-19 2018-12-19
PCT/US2019/067150 WO2020132055A1 (en) 2018-12-19 2019-12-18 Methods for generating and using organoids and tissue therein

Publications (1)

Publication Number Publication Date
SG11202105625UA true SG11202105625UA (en) 2021-07-29

Family

ID=69182679

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202105625UA SG11202105625UA (en) 2018-12-19 2019-12-18 Methods for generating and using organoids and tissue therein

Country Status (10)

Country Link
US (1) US20210301251A1 (en)
EP (1) EP3898944A1 (en)
JP (1) JP2022514298A (en)
KR (1) KR20210105898A (en)
AU (1) AU2019404040A1 (en)
BR (1) BR112021010988A2 (en)
CA (1) CA3119650A1 (en)
MX (1) MX2021007121A (en)
SG (1) SG11202105625UA (en)
WO (1) WO2020132055A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4358980A1 (en) * 2021-06-21 2024-05-01 The Board of Trustees of the Leland Stanford Junior University Multi-regional human neural circuits in assembloids derived from pluripotent stem cells
WO2023044032A1 (en) * 2021-09-16 2023-03-23 Regents Of The University Of Minnesota Multi-tissue organoid products and methods
CN117778313B (en) * 2024-02-23 2024-05-24 成都云测医学生物技术有限公司 Differentiation method and application of mesenchymal stem cells obtained from brain organoids

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0504427D0 (en) * 2005-03-03 2005-04-06 Roslin Inst Edinburgh Method for differentiation of stem cells
US20110082549A1 (en) * 2008-02-01 2011-04-07 Osteosphere, Llc Mineralized three-dimensional bone constructs formed from osteoblasts
JP2011041472A (en) * 2009-08-19 2011-03-03 Tokai Univ Spheroid for cell transplantation treatment composed of cell mixture and method for preparing the same
DK2878664T3 (en) * 2012-07-24 2018-11-05 Nissan Chemical Corp CULTURE MEDIUM COMPOSITION AND PROCEDURE FOR CULTIVATING CELLS OR TISSUE USING THE COMPOSITION
LU92845B1 (en) 2015-10-08 2017-05-02 Univ Du Luxembourg Campus Belval Means and methods for generating midbrain organoids

Also Published As

Publication number Publication date
US20210301251A1 (en) 2021-09-30
AU2019404040A1 (en) 2021-06-03
EP3898944A1 (en) 2021-10-27
KR20210105898A (en) 2021-08-27
MX2021007121A (en) 2021-08-11
CA3119650A1 (en) 2020-06-25
JP2022514298A (en) 2022-02-10
BR112021010988A2 (en) 2021-08-31
WO2020132055A1 (en) 2020-06-25

Similar Documents

Publication Publication Date Title
SG11202000693YA (en) Method and device for generating medical report
IL279393A (en) Bone conduction speaker and testing method therefor
PT3481285T (en) Implants using ultrasonic waves for stimulating tissue
EP3875150C0 (en) Device and cartridge for hifu skincare
EP3542742A4 (en) Customized surgical guide and customized surgical guide generating method and generating program
PT3518783T (en) Apparatus and methods for tissue reduction
SG11202105625UA (en) Methods for generating and using organoids and tissue therein
IL280001A (en) Device for facilitating the formation of new bone tissue
IL259095A (en) Device for isolating cell fractions from human and animal tissues and method for the use thereof
GB2571956B (en) Head-mountable apparatus and methods
IL281233A (en) Automated generation and analysis of organoids
SG11202006666XA (en) An apparatus and a method for the treatment of a prostatic disease
GB201708835D0 (en) Direct tissue analysis
GB2584229B (en) Multi-faceted tissue therapy tool
GB201814516D0 (en) User profile generating system and method
GB201807410D0 (en) Diagnostic method and therapy
EP3244816A4 (en) Apparatus and method for forming an opening in patient's tissue
EP3869490A4 (en) Human body simulation apparatus
GB2572330B (en) Devices and methods for generating electricity
IL280066A (en) Injection techniques for the treatment of cellulite
GB2591964B (en) Surgical apparatus and method
GB2612914B (en) Tissue analysis
EP3815651C0 (en) Medical device and preparation method therefor
IL269817B2 (en) Macro-encapsulated therapeutic cells and methods of using the same
SG11202103815TA (en) Medical device and method for producing the same