SG11202104524YA - Compositions and methods for t cell engineering - Google Patents

Compositions and methods for t cell engineering

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Publication number
SG11202104524YA
SG11202104524YA SG11202104524YA SG11202104524YA SG11202104524YA SG 11202104524Y A SG11202104524Y A SG 11202104524YA SG 11202104524Y A SG11202104524Y A SG 11202104524YA SG 11202104524Y A SG11202104524Y A SG 11202104524YA SG 11202104524Y A SG11202104524Y A SG 11202104524YA
Authority
SG
Singapore
Prior art keywords
compositions
methods
cell engineering
engineering
cell
Prior art date
Application number
SG11202104524YA
Inventor
Xinxin Wang
Tao Jin
Chunhui Yang
Zhongdong Shi
Liping Liu
Jing Sun
Shuyi Qiu
Wei Cao
Original Assignee
Gracell Biotechnologies Shanghai Co Ltd
Suzhou Gracell Biotechnologies Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gracell Biotechnologies Shanghai Co Ltd, Suzhou Gracell Biotechnologies Co Ltd filed Critical Gracell Biotechnologies Shanghai Co Ltd
Publication of SG11202104524YA publication Critical patent/SG11202104524YA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
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SG11202104524YA 2018-11-01 2019-11-01 Compositions and methods for t cell engineering SG11202104524YA (en)

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Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11471490B2 (en) 2017-07-03 2022-10-18 Torque Therapeutics, Inc. T cells surface-loaded with immunostimulatory fusion molecules and uses thereof
TW201934575A (en) * 2018-02-11 2019-09-01 大陸商江蘇恆瑞醫藥股份有限公司 An isolated chimeric antigen receptor and modified T cell contacting same and use thereof
US20200038443A1 (en) * 2018-08-04 2020-02-06 AbCyte Therapeutics Inc. Multi-function and multi-targeting car system and methods for use thereof
KR20220017914A (en) 2019-05-07 2022-02-14 그라셀 바이오테크놀로지스 (상하이) 컴퍼니, 리미티드 Engineered immune cells targeting BCMA and uses thereof
WO2022007784A1 (en) * 2020-07-06 2022-01-13 Nanjing Legend Biotech Co., Ltd. Methods of reducing graft rejection of allogeneic cell therapy
WO2022036495A1 (en) 2020-08-17 2022-02-24 Utc Therapeutics Inc. Lymphocytes-antigen presenting cells co-stimulators and uses thereof
TW202216990A (en) * 2020-09-08 2022-05-01 中國大陸商亘喜生物科技（上海）有限公司 Compositions and methods for t cell engineering
EP4214318A1 (en) * 2020-09-18 2023-07-26 Vor Biopharma Inc. Compositions and methods for cd7 modification
KR20230118887A (en) * 2020-12-03 2023-08-14 센츄리 쎄라퓨틱스 인코포레이티드 Genetically Engineered Cells and Uses Thereof
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
WO2022125439A2 (en) * 2020-12-07 2022-06-16 The Regents Of The University Of California Innate immune cell silencing by sirp-alpha engager
TW202241935A (en) 2020-12-18 2022-11-01 美商世紀治療股份有限公司 Chimeric antigen receptor system with adaptable receptor specificity
CN116648457A (en) * 2020-12-31 2023-08-25 亘喜生物科技（上海）有限公司 Membrane fusion proteins and their use in immune cells
CN116964083A (en) * 2021-03-04 2023-10-27 艾洛基治疗公司 FasL expression and FasR gene knockout for protecting therapeutic cells from allograft rejection and activation-induced cell death
CN112980800A (en) * 2021-03-08 2021-06-18 河北森朗生物科技有限公司 CAR-T cell, construction method and application thereof
BR112023018844A2 (en) * 2021-04-07 2023-10-10 Century Therapeutics Inc COMPOSITIONS AND METHODS FOR GENERATION OF GAMMA-DELTA T CELLS FROM INDUCED PLURIPOTENT STEM CELLS
WO2023283644A2 (en) * 2021-07-09 2023-01-12 Board Of Regents, The University Of Texas System Chimeric antigen receptor to target trop-2-positive cancers
CN113603778A (en) * 2021-08-06 2021-11-05 上海优卡迪生物医药科技有限公司 Blocking antibody and application thereof in preparation of targeting T cell expression antigen CAR-T cell
TW202325744A (en) * 2021-09-29 2023-07-01 美商莫德斯醫療公司 Antigen binding polypeptides, antigen binding polypeptide complexes and methods of use thereof in hiv
CN115873801A (en) * 2021-09-29 2023-03-31 南京北恒生物科技有限公司 Engineered immune cells and uses thereof
CN113925876B (en) * 2021-10-14 2022-08-12 范德里希(上海)生物科技有限公司 Use of CIK immune cells in the treatment of cancer
WO2023086379A2 (en) * 2021-11-10 2023-05-19 TCR2 Therapeutics Inc. Compositions and methods for tcr reprogramming using fusion proteins
CN114456262B (en) * 2022-01-13 2024-06-11 浙江大学医学院附属第一医院 Anti-H1N 1 influenza virus nucleoprotein monoclonal antibody ZJU-NP-A1 and application thereof in detection
WO2023150544A1 (en) * 2022-02-01 2023-08-10 Seattle Children's Hospital D/B/A Seattle Children's Research Institute Simplified method of preparing cells for patient administration
WO2023192948A1 (en) * 2022-04-01 2023-10-05 Seattle Children's Hospital (dba Seattle Children's Research Institute) Chimeric cytokine receptors
CN116640226B (en) * 2022-04-02 2024-04-26 广东东阳光药业股份有限公司 Chimeric receptor for inhibiting host anti-exogenous immune cell HVG reaction
WO2023197980A1 (en) * 2022-04-11 2023-10-19 苏州沙砾生物科技有限公司 Chimeric antigen receptor and use thereof
CN117004603A (en) * 2022-04-28 2023-11-07 南京北恒生物科技有限公司 Engineered immune cell with CD7 gene knocked out and application thereof
WO2024019961A1 (en) * 2022-07-18 2024-01-25 Cargo Therapeutics, Inc. Cd2 recruiting chimeric antigen receptors and fusion proteins
CN115838423B (en) * 2022-08-16 2023-05-09 北京科跃中楷生物技术有限公司 Cell rapid fusion technology and antibody prepared by same
CN117683139A (en) * 2022-09-09 2024-03-12 信达细胞制药(苏州)有限公司 Constitutive chimeric cytokine receptor, immune cell expressing same and application thereof
CN115960226B (en) * 2022-09-20 2023-06-16 北京科跃中楷生物技术有限公司 Magnetic particle immunochromatography rapid reaction method and detection kit
CN116179483B (en) * 2022-12-16 2024-03-01 青岛海尔生物科技有限公司 Method for rapidly amplifying stem cell-like memory cervical cancer tumor infiltrating lymphocytes in vitro
CN117402231B (en) * 2023-12-14 2024-04-09 南方医科大学南方医院 Receptor capable of spontaneously transmitting IL-21 signal and application thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR901228A (en) 1943-01-16 1945-07-20 Deutsche Edelstahlwerke Ag Ring gap magnet system
US2779780A (en) 1955-03-01 1957-01-29 Du Pont 1, 4-diamino-2, 3-dicyano-1, 4-bis (substituted mercapto) butadienes and their preparation
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6995162B2 (en) 2001-01-12 2006-02-07 Amgen Inc. Substituted alkylamine derivatives and methods of use
EP1478648B1 (en) 2002-02-01 2014-04-30 ARIAD Pharmaceuticals, Inc. Phosphorus-containing compounds and uses thereof
TWI275390B (en) 2002-04-30 2007-03-11 Wyeth Corp Process for the preparation of 7-substituted-3- quinolinecarbonitriles
AU2013221672B2 (en) 2012-02-13 2017-11-09 Seattle Children's Hospital D/B/A Seattle Children's Research Institute Bispecific chimeric antigen receptors and therapeutic uses thereof
EP3943507A1 (en) 2014-04-10 2022-01-26 Seattle Children's Hospital, dba Seattle Children's Research Institute Drug related transgene expression
CN106191062B (en) * 2016-07-18 2019-06-14 广东华南疫苗股份有限公司 A kind of TCR-/PD-1- double negative t cells and its construction method
KR20230167769A (en) 2016-08-26 2023-12-11 베이롤 칼리지 오브 메드신 Constitutively active cytokine receptors for cell therapy
CN106544321B (en) * 2016-10-13 2019-01-29 北京艺妙神州医疗科技有限公司 Universal CAR-T cell and its preparation method and application
CN106591363A (en) * 2016-11-11 2017-04-26 广东万海细胞生物科技有限公司 Preparation method of universal heterologous CAR-T cells and application
GB201622044D0 (en) 2016-12-22 2017-02-08 Ucl Business Plc T cell-targeted T cells

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