SG11202101900PA - Inducible expression system for plasmid-free production of a protein of interest - Google Patents

Inducible expression system for plasmid-free production of a protein of interest

Info

Publication number
SG11202101900PA
SG11202101900PA SG11202101900PA SG11202101900PA SG11202101900PA SG 11202101900P A SG11202101900P A SG 11202101900PA SG 11202101900P A SG11202101900P A SG 11202101900PA SG 11202101900P A SG11202101900P A SG 11202101900PA SG 11202101900P A SG11202101900P A SG 11202101900PA
Authority
SG
Singapore
Prior art keywords
plasmid
interest
protein
expression system
inducible expression
Prior art date
Application number
SG11202101900PA
Inventor
Artur Schuller
Monika Cserjan
Reingard Grabherr
Gerald Striedner
Original Assignee
Boehringer Ingelheim Rcv Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Rcv Gmbh filed Critical Boehringer Ingelheim Rcv Gmbh
Publication of SG11202101900PA publication Critical patent/SG11202101900PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
SG11202101900PA 2018-09-11 2019-09-11 Inducible expression system for plasmid-free production of a protein of interest SG11202101900PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18193655 2018-09-11
PCT/EP2019/074239 WO2020053285A1 (en) 2018-09-11 2019-09-11 Inducible expression system for plasmid-free production of a protein of interest

Publications (1)

Publication Number Publication Date
SG11202101900PA true SG11202101900PA (en) 2021-04-29

Family

ID=63708078

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202101900PA SG11202101900PA (en) 2018-09-11 2019-09-11 Inducible expression system for plasmid-free production of a protein of interest

Country Status (9)

Country Link
US (1) US20220049260A1 (en)
EP (1) EP3850101A1 (en)
JP (1) JP2022500036A (en)
KR (1) KR20210057751A (en)
CN (1) CN112689676A (en)
AU (1) AU2019337392A1 (en)
CA (1) CA3111365A1 (en)
SG (1) SG11202101900PA (en)
WO (1) WO2020053285A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115011625A (en) * 2022-06-02 2022-09-06 山东大学 IPTG (isopropyl thiogalactoside) inducible promoter vector for Acidithiobacillus caldus and application of IPTG inducible promoter vector

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002348205A1 (en) 2001-12-12 2003-06-23 Eli Lilly And Company Expression system
WO2008127997A1 (en) * 2007-04-12 2008-10-23 Wisconsin Alumni Research Foundation Enhanced protein expression using auto-induction media
JP5837747B2 (en) * 2007-05-17 2015-12-24 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト Method for producing recombinant protein on an industrial scale
CN101993878B (en) * 2010-10-14 2012-04-18 南京农业大学 RRNA mosaic promoter and expression vector containing same
CN103276005B (en) * 2013-05-07 2015-06-10 清华大学 Recombinant plasmid based on T7 expression system
CN103361345B (en) * 2013-06-15 2016-05-04 福州大学 The biosynthetic method of the biological components and parts strengthening secondary metabolite of restructuring regulation and control

Also Published As

Publication number Publication date
EP3850101A1 (en) 2021-07-21
AU2019337392A1 (en) 2021-03-11
CA3111365A1 (en) 2020-03-19
KR20210057751A (en) 2021-05-21
US20220049260A1 (en) 2022-02-17
CN112689676A (en) 2021-04-20
WO2020053285A1 (en) 2020-03-19
JP2022500036A (en) 2022-01-04

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