SG11202001029WA - Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells - Google Patents
Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cellsInfo
- Publication number
- SG11202001029WA SG11202001029WA SG11202001029WA SG11202001029WA SG11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA
- Authority
- SG
- Singapore
- Prior art keywords
- nanostraws
- apparatuses
- methods
- adherent cells
- biologically relevant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2535/00—Supports or coatings for cell culture characterised by topography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Electromagnetism (AREA)
- Mechanical Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762534511P | 2017-07-19 | 2017-07-19 | |
PCT/US2018/042516 WO2019018415A1 (en) | 2017-07-19 | 2018-07-17 | Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11202001029WA true SG11202001029WA (en) | 2020-03-30 |
Family
ID=65015878
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11202001029WA SG11202001029WA (en) | 2017-07-19 | 2018-07-17 | Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells |
Country Status (7)
Country | Link |
---|---|
US (2) | US10815499B2 (en) |
EP (1) | EP3655159B1 (en) |
JP (1) | JP7104140B2 (en) |
AU (1) | AU2018304182B2 (en) |
CA (1) | CA3070413A1 (en) |
SG (1) | SG11202001029WA (en) |
WO (1) | WO2019018415A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3512802B1 (en) | 2016-09-13 | 2021-07-21 | The Board of Trustees of the Leland Stanford Junior University | Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring |
AU2018304182B2 (en) | 2017-07-19 | 2023-04-13 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells |
US20230348938A1 (en) * | 2020-08-14 | 2023-11-02 | Navan Technologies, Inc. | Methods and apparatuses for biomolecule delivery to primary human hematopoietic stem cells using nanostraws |
Family Cites Families (37)
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EP0962524B1 (en) | 1998-05-27 | 2004-11-03 | Micronas GmbH | Apparatus and process for the intracellular manipulation of a biological cell |
DE19841337C1 (en) | 1998-05-27 | 1999-09-23 | Micronas Intermetall Gmbh | Intracellular manipulation of biological cell contents, assisting injection or removal of substances or cell components |
JP2003501639A (en) | 1999-06-03 | 2003-01-14 | ユニバーシティ オブ ワシントン | Microfluidic devices for transverse and isoelectric focusing |
US6403348B1 (en) | 1999-07-21 | 2002-06-11 | The Regents Of The University Of California | Controlled electroporation and mass transfer across cell membranes |
WO2002058847A2 (en) | 2000-11-28 | 2002-08-01 | Georgetown University | Cell transformation using a single chip silicon microfabricated array incorporating integrated micro-piercing injectors |
WO2008072029A2 (en) | 2002-02-12 | 2008-06-19 | Cellectricon Ab | Systems and methods for rapidly changing the solution environment around sensors |
WO2004036202A1 (en) | 2002-10-16 | 2004-04-29 | Cellectricon Ab | Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells |
JP2006508790A (en) | 2002-12-04 | 2006-03-16 | スピンクス インコーポレイテッド | Apparatus and method for programmable microanalytical scale manipulation of fluids |
US7355216B2 (en) | 2002-12-09 | 2008-04-08 | The Regents Of The University Of California | Fluidic nanotubes and devices |
CN1286716C (en) | 2003-03-19 | 2006-11-29 | 清华大学 | Method for growing carbon nano tube |
US7444856B2 (en) | 2004-09-23 | 2008-11-04 | The Board Of Trustees Of The Leland Stanford Junior University | Sensors for electrochemical, electrical or topographical analysis |
EP1879522A2 (en) | 2005-04-28 | 2008-01-23 | The Regents of The University of California | Compositions comprising nanostructures for cell, tissue and artificial organ growth, and methods for making and using same |
KR100672872B1 (en) | 2005-10-28 | 2007-01-24 | 한국과학기술연구원 | Fabrication method of three dimensional nanostructure |
US20100215724A1 (en) | 2005-11-22 | 2010-08-26 | Mcgill University | Microcapsule Nanotube Devices for Targeted Delivery of Therapeutic Molecules |
US20100068461A1 (en) | 2006-06-30 | 2010-03-18 | University Of Wollongong | Nanostructured composites |
ITFI20060295A1 (en) | 2006-11-24 | 2008-05-25 | Scuola Superiore Di Studi Universitari E Di ... | METHOD OF NON-INVASIVE ELECTROPORATION MEDIATED BY CARBON NANOTUBES AND DEVICE FOR IMPLEMENTING THIS METHOD. |
US20120264108A1 (en) | 2007-01-05 | 2012-10-18 | The Regents Of The University Of California | Intracellular Molecular Delivery Based On Nanostructure Injectors |
DE102007019842A1 (en) | 2007-04-25 | 2008-10-30 | Forschungsinstitut Für Die Biologie Landwirtschaftlicher Nutztiere | Method and arrangement for electrically contacting a membrane-encased object with an electrode |
US7870616B2 (en) | 2007-05-11 | 2011-01-11 | Csem Centre Suisse D'electronique Et De Microtechnique Sa | Probe arrangement |
WO2009063776A1 (en) | 2007-11-02 | 2009-05-22 | Humanix Co., Ltd. | Method of capturing cell fluid and analyzing components thereof while observing cell and system for capturing and analyzing cell fluid |
WO2010121130A2 (en) | 2009-04-16 | 2010-10-21 | President And Fellows Of Harvard College | Molecular delivery with nanowires |
WO2011050009A1 (en) | 2009-10-19 | 2011-04-28 | Rational Biotechnology Inc. | Method, device and apparatus for inducing self-adjusting cell electroporation |
US8661663B2 (en) | 2010-02-22 | 2014-03-04 | University Of Houston | Method for manufacturing a multimodal neural probe |
DE102011015184B4 (en) | 2010-06-02 | 2013-11-21 | Thinxxs Microtechnology Ag | Device for transporting small volumes of a fluid, in particular micropump or microvalve |
JP6151644B2 (en) | 2011-03-03 | 2017-06-21 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Operation device, deposition method, injection method, detection device, and detection method |
US8808516B2 (en) | 2011-03-04 | 2014-08-19 | The Board Of Trustees Of The Leland Stanford Junior University | Devices and methods for long-term intracellular access |
US9856448B2 (en) | 2011-03-04 | 2018-01-02 | The Board Of Trustees Of The Leland Stanford Junior Univesity | Devices and methods for long-term intracellular access |
US9266725B2 (en) | 2011-04-27 | 2016-02-23 | The Board Of Trustees Of The Leland Stanford Junior University | Nanotube structures, methods of making nanotube structures, and methods of accessing intracellular space |
WO2013086502A1 (en) | 2011-12-09 | 2013-06-13 | President And Fellows Of Harvard College | Organ chips and uses thereof |
WO2014031171A1 (en) * | 2012-08-20 | 2014-02-27 | President And Fellows Of Harvard College | Use of nanowires for delivering biological effectors into immune cells |
US10696962B2 (en) | 2013-03-14 | 2020-06-30 | The Regents Of The University Of California | Nanopipette device and method for subcellular analysis |
WO2017027549A1 (en) | 2015-08-10 | 2017-02-16 | Duke University | Magnetic single cell arrays for probing cell-drug and cell-cell communication |
KR20190019129A (en) | 2016-06-09 | 2019-02-26 | 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 | Nanostrowell insert devices for improved cell transfection and survival rates |
EP3512802B1 (en) | 2016-09-13 | 2021-07-21 | The Board of Trustees of the Leland Stanford Junior University | Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring |
AU2018304182B2 (en) | 2017-07-19 | 2023-04-13 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells |
US20190365803A1 (en) | 2018-06-04 | 2019-12-05 | Nicholas A. Melosh | Nanostraws methods and apparatuses |
US20230348938A1 (en) * | 2020-08-14 | 2023-11-02 | Navan Technologies, Inc. | Methods and apparatuses for biomolecule delivery to primary human hematopoietic stem cells using nanostraws |
-
2018
- 2018-07-17 AU AU2018304182A patent/AU2018304182B2/en active Active
- 2018-07-17 EP EP18835094.6A patent/EP3655159B1/en active Active
- 2018-07-17 WO PCT/US2018/042516 patent/WO2019018415A1/en unknown
- 2018-07-17 JP JP2020502603A patent/JP7104140B2/en active Active
- 2018-07-17 SG SG11202001029WA patent/SG11202001029WA/en unknown
- 2018-07-17 CA CA3070413A patent/CA3070413A1/en active Pending
- 2018-07-17 US US16/038,062 patent/US10815499B2/en active Active
-
2020
- 2020-10-27 US US17/081,983 patent/US11999966B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2020527948A (en) | 2020-09-17 |
US11999966B2 (en) | 2024-06-04 |
CA3070413A1 (en) | 2019-01-24 |
EP3655159A4 (en) | 2021-04-21 |
EP3655159B1 (en) | 2022-09-14 |
EP3655159A1 (en) | 2020-05-27 |
WO2019018415A1 (en) | 2019-01-24 |
US20210062222A1 (en) | 2021-03-04 |
JP7104140B2 (en) | 2022-07-20 |
US10815499B2 (en) | 2020-10-27 |
US20190024122A1 (en) | 2019-01-24 |
AU2018304182A1 (en) | 2020-02-06 |
AU2018304182B2 (en) | 2023-04-13 |
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