SG11202001029WA - Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells - Google Patents

Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells

Info

Publication number
SG11202001029WA
SG11202001029WA SG11202001029WA SG11202001029WA SG11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA SG 11202001029W A SG11202001029W A SG 11202001029WA
Authority
SG
Singapore
Prior art keywords
nanostraws
apparatuses
methods
adherent cells
biologically relevant
Prior art date
Application number
SG11202001029WA
Inventor
Karl Martin Hjort
Sergio Leal-Ortiz
Yuhong Cao
Chris Rehse
Andy Tay
Nicholas Melosh
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Publication of SG11202001029WA publication Critical patent/SG11202001029WA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Mechanical Engineering (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Electromagnetism (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202001029WA 2017-07-19 2018-07-17 Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells SG11202001029WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762534511P 2017-07-19 2017-07-19
PCT/US2018/042516 WO2019018415A1 (en) 2017-07-19 2018-07-17 Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells

Publications (1)

Publication Number Publication Date
SG11202001029WA true SG11202001029WA (en) 2020-03-30

Family

ID=65015878

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202001029WA SG11202001029WA (en) 2017-07-19 2018-07-17 Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells

Country Status (7)

Country Link
US (2) US10815499B2 (en)
EP (1) EP3655159B1 (en)
JP (1) JP7104140B2 (en)
AU (1) AU2018304182B2 (en)
CA (1) CA3070413A1 (en)
SG (1) SG11202001029WA (en)
WO (1) WO2019018415A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11149266B2 (en) 2016-09-13 2021-10-19 The Board Of Trustees Of The Leland Stanford Junior University Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring
SG11202001029WA (en) 2017-07-19 2020-03-30 Univ Leland Stanford Junior Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells
EP4196260A1 (en) * 2020-08-14 2023-06-21 Navan Technologies, Inc. Methods and apparatuses for biomolecule delivery to primary human hematopoietic stem cells using nanostraws

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EP0962524B1 (en) 1998-05-27 2004-11-03 Micronas GmbH Apparatus and process for the intracellular manipulation of a biological cell
WO2000074850A2 (en) 1999-06-03 2000-12-14 University Of Washington Microfluidic devices for transverse electrophoresis and isoelectric focusing
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AU2002246527A1 (en) 2000-11-28 2002-08-06 Georgetown University Cell transformation using a single chip silicon microfabricated array incorporating integrated micro-piercing injectors
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US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
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EP1879522A2 (en) 2005-04-28 2008-01-23 The Regents of The University of California Compositions comprising nanostructures for cell, tissue and artificial organ growth, and methods for making and using same
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WO2008000045A1 (en) 2006-06-30 2008-01-03 University Of Wollongong Nanostructured composites
ITFI20060295A1 (en) * 2006-11-24 2008-05-25 Scuola Superiore Di Studi Universitari E Di ... METHOD OF NON-INVASIVE ELECTROPORATION MEDIATED BY CARBON NANOTUBES AND DEVICE FOR IMPLEMENTING THIS METHOD.
WO2008133755A2 (en) * 2007-01-05 2008-11-06 The Regents Of The University Of California Intracellular molecular delivery based on nanostructure injectors
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US9856448B2 (en) 2011-03-04 2018-01-02 The Board Of Trustees Of The Leland Stanford Junior Univesity Devices and methods for long-term intracellular access
US8808516B2 (en) 2011-03-04 2014-08-19 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for long-term intracellular access
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US11149266B2 (en) 2016-09-13 2021-10-19 The Board Of Trustees Of The Leland Stanford Junior University Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring
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EP4196260A1 (en) * 2020-08-14 2023-06-21 Navan Technologies, Inc. Methods and apparatuses for biomolecule delivery to primary human hematopoietic stem cells using nanostraws

Also Published As

Publication number Publication date
US20190024122A1 (en) 2019-01-24
JP2020527948A (en) 2020-09-17
JP7104140B2 (en) 2022-07-20
US20210062222A1 (en) 2021-03-04
EP3655159B1 (en) 2022-09-14
AU2018304182B2 (en) 2023-04-13
US10815499B2 (en) 2020-10-27
EP3655159A4 (en) 2021-04-21
EP3655159A1 (en) 2020-05-27
US11999966B2 (en) 2024-06-04
AU2018304182A1 (en) 2020-02-06
WO2019018415A1 (en) 2019-01-24
CA3070413A1 (en) 2019-01-24

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