SG11201800503YA - Stem cell culturing method - Google Patents

Stem cell culturing method

Info

Publication number
SG11201800503YA
SG11201800503YA SG11201800503YA SG11201800503YA SG11201800503YA SG 11201800503Y A SG11201800503Y A SG 11201800503YA SG 11201800503Y A SG11201800503Y A SG 11201800503YA SG 11201800503Y A SG11201800503Y A SG 11201800503YA SG 11201800503Y A SG11201800503Y A SG 11201800503YA
Authority
SG
Singapore
Prior art keywords
stem cell
cell culturing
culturing method
stem
culturing
Prior art date
Application number
SG11201800503YA
Inventor
Alexei GLADKOV
Original Assignee
Kintarocellspower Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kintarocellspower Co Ltd filed Critical Kintarocellspower Co Ltd
Priority claimed from PCT/JP2016/086206 external-priority patent/WO2017099067A1/en
Publication of SG11201800503YA publication Critical patent/SG11201800503YA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00Tissue, human, animal or plant cell, or virus culture apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/05Means for pre-treatment of biological substances by centrifugation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02Separating microorganisms from the culture medium; Concentration of biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00Cells for large scale production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11201800503YA 2015-12-07 2016-12-06 Stem cell culturing method SG11201800503YA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015238349 2015-12-07
JP2016218144A JP6153653B2 (en) 2015-12-07 2016-11-08 Stem cell culture method
PCT/JP2016/086206 WO2017099067A1 (en) 2015-12-07 2016-12-06 Stem cell culturing method

Publications (1)

Publication Number Publication Date
SG11201800503YA true SG11201800503YA (en) 2018-02-27

Family

ID=59058006

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201800503YA SG11201800503YA (en) 2015-12-07 2016-12-06 Stem cell culturing method

Country Status (8)

Country Link
US (1) US20180291346A1 (en)
EP (1) EP3315603A4 (en)
JP (1) JP6153653B2 (en)
CN (1) CN108350426A (en)
HK (1) HK1254737A1 (en)
PH (1) PH12018500160A1 (en)
RU (1) RU2732238C2 (en)
SG (1) SG11201800503YA (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2751239C2 (en) * 2016-03-10 2021-07-12 Кинтароселлспауэр Ко., Лтд. Method for producing liquid culture product

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003513648A (en) * 1999-10-29 2003-04-15 フイラデルフイア・ヘルス・アンド・エデユケーシヨン・コーポレーシヨン Isolation and expansion of human bone marrow stromal cells
DE10336152B4 (en) * 2003-08-06 2007-02-15 Nephrogen LLC, Salt Lake City Purification method for human mesenchymal stem cells
JP4542409B2 (en) * 2004-10-14 2010-09-15 オリンパス株式会社 Method for culturing bone marrow-derived mesenchymal stem cells
CN1699557A (en) * 2005-05-26 2005-11-23 南开大学 Process for separation and culture of marrow mesenchyme stem cell
SG183570A1 (en) * 2010-03-10 2012-10-30 Two Cells Co Ltd Cell preparation containing mesenchymal stem cells, and method for producing same
CN102140440A (en) * 2011-04-27 2011-08-03 中国农业大学 Method for separating and culturing poultry bone mesenchymal stem cells by using bone marrow tissue block
RU2502999C1 (en) * 2012-07-25 2013-12-27 Общество с ограниченной ответственностью Научно-Производственное Объединение "Тюменькриобанк" Method for producing viable heterogenous skin cell population
JP6452695B2 (en) * 2013-08-06 2019-01-16 リジェネックス, エルエルシー Bone marrow fat portion isolation device and method
CN103436491B (en) * 2013-09-05 2015-10-28 东南大学 A kind of based on the cell density at the bottom of insect wing base and the method arranging controlled cultivation
JP2015084686A (en) * 2013-10-29 2015-05-07 オリンパス株式会社 Jig for cell culture, and automatic culture medium exchange system
JP6628253B2 (en) * 2014-02-11 2020-01-08 ブレインストーム セル セラペウティクス リミテッド Method for determining cell suitability
CN104152409B (en) * 2014-08-18 2017-05-03 云南农业大学 Method for simultaneous isolated culture of canine bone marrow mesenchymal stem cells and multifunctional hematopoietic stem cells

Also Published As

Publication number Publication date
CN108350426A (en) 2018-07-31
JP6153653B2 (en) 2017-06-28
HK1254737A1 (en) 2019-07-26
RU2018107552A (en) 2020-01-10
US20180291346A1 (en) 2018-10-11
EP3315603A4 (en) 2019-04-03
RU2732238C2 (en) 2020-09-14
RU2018107552A3 (en) 2020-04-15
EP3315603A1 (en) 2018-05-02
PH12018500160A1 (en) 2018-08-13
JP2017104094A (en) 2017-06-15

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