SG11201706636PA - Single-cell nucleic acids for high-throughput studies - Google Patents
Single-cell nucleic acids for high-throughput studiesInfo
- Publication number
- SG11201706636PA SG11201706636PA SG11201706636PA SG11201706636PA SG11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acids
- cell nucleic
- throughput studies
- throughput
- studies
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Fluid Mechanics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562126349P | 2015-02-27 | 2015-02-27 | |
| PCT/US2016/019952 WO2016138490A1 (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201706636PA true SG11201706636PA (en) | 2017-09-28 |
Family
ID=56789110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201706636PA SG11201706636PA (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Country Status (7)
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| SG174617A1 (en) | 2009-04-02 | 2011-10-28 | Fluidigm Corp | Multi-primer amplification method for barcoding of target nucleic acids |
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| CN112592960B (zh) | 2011-05-20 | 2024-08-27 | 富鲁达公司 | 核酸编码反应 |
| CN103998394B (zh) | 2011-08-01 | 2016-08-17 | 德诺弗科学公司 | 细胞捕获系统和使用方法 |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| US9606102B2 (en) | 2013-01-26 | 2017-03-28 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| CN107250445A (zh) | 2015-02-27 | 2017-10-13 | 富鲁达公司 | 用于高通量研究的单个细胞核酸 |
| AU2016348439B2 (en) | 2015-11-04 | 2023-03-09 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| EP3390658B1 (en) | 2015-12-16 | 2022-08-03 | Standard BioTools Inc. | High-level multiplex amplification |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CA3041645C (en) | 2016-10-24 | 2021-11-02 | Geneinfosec, Inc. | Concealing information present within nucleic acids |
| US11753743B2 (en) | 2017-02-13 | 2023-09-12 | Yale University | High-throughput single-cell polyomics |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| KR102683229B1 (ko) * | 2017-06-20 | 2024-07-08 | 소마젠 인크 | 고유 분자 식별자를 갖는 라이브러리 제조 방법 및 시스템 |
| CN112980667B (zh) | 2017-08-29 | 2024-09-17 | 伯乐实验室有限公司 | 用于分离和分析细胞的系统和方法 |
| WO2019046564A1 (en) * | 2017-08-30 | 2019-03-07 | Kapa Biosystems, Inc. | TRANSPOSASE COMPOSITIONS, PREPARATION METHODS, AND SCREENING METHODS |
| CN112236518A (zh) * | 2018-02-23 | 2021-01-15 | 耶鲁大学 | 单细胞冻融裂解 |
| EP3837380A4 (en) * | 2018-08-17 | 2022-05-11 | Cellecta, Inc. | MULTIPLEX PREPARATION OF BARCODE GENE-SPECIFIC DNA FRAGMENTS |
| CN112805389B (zh) * | 2018-10-01 | 2024-08-06 | 贝克顿迪金森公司 | 确定5’转录物序列 |
| CN120099139A (zh) | 2019-02-14 | 2025-06-06 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| SG11202112151UA (en) | 2019-05-07 | 2021-12-30 | Bio Rad Laboratories | System and method for automated single cell processing |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| US11724256B2 (en) | 2019-06-14 | 2023-08-15 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing and analyses |
| US20220333194A1 (en) * | 2019-08-08 | 2022-10-20 | Inserm (Institutnationaldelasanteetdelarecherchemedicale) | Rna sequencing method for the analysis of b and t cell transcriptome in phenotypically defined b and t cell subsets |
| CN114729392A (zh) * | 2019-09-06 | 2022-07-08 | 10X基因组学有限公司 | 用于对细胞和细胞珠粒进行条形码化的系统和方法 |
| CN114555824A (zh) * | 2019-10-11 | 2022-05-27 | 布莱克霍克基因组学有限责任公司 | 使用长读测序鉴定基因组结构变体 |
| GB2589869A (en) * | 2019-12-09 | 2021-06-16 | Univ Oxford Innovation Ltd | Method for whole genome sequencing of picogram quantities of DNA |
| US12188010B2 (en) | 2020-01-29 | 2025-01-07 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| EP4240842B1 (en) * | 2020-11-03 | 2024-12-18 | ACT Genomics (IP) Limited | Targeted sequencing method and kit thereof for detecting gene alteration |
| EP4263859A1 (en) | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
| US20240401026A1 (en) | 2021-09-13 | 2024-12-05 | Ecole Polytechnique Federale De Lausanne (Epfl) | System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies |
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| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
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| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
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| SG183029A1 (en) | 2009-01-13 | 2012-08-30 | Fluidigm Corp | Single-cell nucleic acid analysis |
| US8450063B2 (en) | 2009-01-28 | 2013-05-28 | Fluidigm Corporation | Determination of copy number differences by amplification |
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| EP2569453B1 (en) | 2010-05-14 | 2015-12-16 | Fluidigm Corporation | Nucleic acid isolation methods |
| EP2569447A4 (en) | 2010-05-14 | 2013-11-27 | Fluidigm Corp | ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY |
| CN112592960B (zh) | 2011-05-20 | 2024-08-27 | 富鲁达公司 | 核酸编码反应 |
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| CN104471077B (zh) | 2012-05-21 | 2017-05-24 | 富鲁达公司 | 颗粒群的单颗粒分析 |
| WO2014031954A1 (en) * | 2012-08-23 | 2014-02-27 | Tufts University | Homopolymer mediated nucleic acid amplification |
| US20160122753A1 (en) * | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| CN107250445A (zh) | 2015-02-27 | 2017-10-13 | 富鲁达公司 | 用于高通量研究的单个细胞核酸 |
| EP3390658B1 (en) | 2015-12-16 | 2022-08-03 | Standard BioTools Inc. | High-level multiplex amplification |
-
2016
- 2016-02-26 CN CN201680012542.8A patent/CN107250445A/zh active Pending
- 2016-02-26 EP EP16756523.3A patent/EP3262214B1/en active Active
- 2016-02-26 US US15/055,252 patent/US10190163B2/en active Active
- 2016-02-26 WO PCT/US2016/019952 patent/WO2016138490A1/en active Application Filing
- 2016-02-26 SG SG11201706636PA patent/SG11201706636PA/en unknown
- 2016-02-26 JP JP2017542907A patent/JP2018509896A/ja active Pending
- 2016-02-26 CA CA2976681A patent/CA2976681A1/en active Pending
-
2018
- 2018-12-10 US US16/215,489 patent/US10954560B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US10190163B2 (en) | 2019-01-29 |
| US20190185929A1 (en) | 2019-06-20 |
| WO2016138490A1 (en) | 2016-09-01 |
| CN107250445A (zh) | 2017-10-13 |
| US10954560B2 (en) | 2021-03-23 |
| EP3262214A4 (en) | 2018-07-25 |
| CA2976681A1 (en) | 2016-09-01 |
| US20160251714A1 (en) | 2016-09-01 |
| EP3262214A1 (en) | 2018-01-03 |
| JP2018509896A (ja) | 2018-04-12 |
| EP3262214B1 (en) | 2023-10-25 |
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