SG11201706636PA - Single-cell nucleic acids for high-throughput studies - Google Patents
Single-cell nucleic acids for high-throughput studiesInfo
- Publication number
- SG11201706636PA SG11201706636PA SG11201706636PA SG11201706636PA SG11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA SG 11201706636P A SG11201706636P A SG 11201706636PA
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acids
- cell nucleic
- throughput studies
- throughput
- studies
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Fluid Mechanics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562126349P | 2015-02-27 | 2015-02-27 | |
| PCT/US2016/019952 WO2016138490A1 (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201706636PA true SG11201706636PA (en) | 2017-09-28 |
Family
ID=56789110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201706636PA SG11201706636PA (en) | 2015-02-27 | 2016-02-26 | Single-cell nucleic acids for high-throughput studies |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10190163B2 (de) |
| EP (1) | EP3262214B1 (de) |
| JP (1) | JP2018509896A (de) |
| CN (1) | CN107250445A (de) |
| CA (1) | CA2976681A1 (de) |
| SG (1) | SG11201706636PA (de) |
| WO (1) | WO2016138490A1 (de) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8691509B2 (en) | 2009-04-02 | 2014-04-08 | Fluidigm Corporation | Multi-primer amplification method for barcoding of target nucleic acids |
| SG194745A1 (en) | 2011-05-20 | 2013-12-30 | Fluidigm Corp | Nucleic acid encoding reactions |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| WO2013019491A1 (en) | 2011-08-01 | 2013-02-07 | Denovo Sciences | Cell capture system and method of use |
| US9606102B2 (en) | 2013-01-26 | 2017-03-28 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| CN107250445A (zh) | 2015-02-27 | 2017-10-13 | 富鲁达公司 | 用于高通量研究的单个细胞核酸 |
| CN108473984A (zh) | 2015-11-04 | 2018-08-31 | 阿特雷卡公司 | 用于分析与单个细胞相关联的核酸的核酸条形码的组合组 |
| US11117113B2 (en) | 2015-12-16 | 2021-09-14 | Fluidigm Corporation | High-level multiplex amplification |
| IL266197B2 (en) | 2016-10-24 | 2024-03-01 | Geneinfosec Inc | Hiding information contained within nucleic acids |
| WO2018148700A1 (en) * | 2017-02-13 | 2018-08-16 | Yale University | High-throughput single-cell polyomics |
| EP3651903A4 (de) | 2017-08-29 | 2021-06-16 | Bio-Rad Laboratories, Inc. | System und verfahren zur isolierung und analyse von zellen |
| EP3676396B1 (de) * | 2017-08-30 | 2022-04-06 | Kapa Biosystems, Inc. | Transposasezusammensetzungen, verfahren zur herstellung und verfahren zum screening |
| WO2019165181A1 (en) * | 2018-02-23 | 2019-08-29 | Yale University | Single-cell freeze-thaw lysis |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| AU2020267743B2 (en) | 2019-05-07 | 2023-04-06 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| EP3982716A4 (de) | 2019-06-14 | 2023-09-06 | Bio-Rad Laboratories, Inc. | System und verfahren für automatisierte einzelzellenverarbeitung und -analyse |
| JP2022544101A (ja) * | 2019-08-08 | 2022-10-17 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | 表現型の規定されたｂ細胞及びｔ細胞のサブセットにおける、ｂ細胞とｔ細胞のトランスクリプトームの分析のためのｒｎａシークエンス法 |
| EP4025709A1 (de) * | 2019-09-06 | 2022-07-13 | 10X Genomics, Inc. | Systeme und verfahren zum barcodieren von zellen und zellwulsten |
| GB2589869A (en) * | 2019-12-09 | 2021-06-16 | Univ Oxford Innovation Ltd | Method for whole genome sequencing of picogram quantities of DNA |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2023037334A1 (en) | 2021-09-13 | 2023-03-16 | Ecole Polytechnique Federale De Lausanne (Epfl) | System and method for single cell phenotypical profiling and deterministic nanoliter-droplet encapsulation and deterministic droplet consortia assemblies |
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| US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| EP0920440B1 (de) | 1996-02-09 | 2012-08-22 | Cornell Research Foundation, Inc. | Detektion von unterschieden in der sequenz von nukleinsäuren mittels der ligasedetektionsreaktion und adressierbaren anordnungen |
| CA2255774C (en) | 1996-05-29 | 2008-03-18 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US6770748B2 (en) | 1997-03-07 | 2004-08-03 | Takeshi Imanishi | Bicyclonucleoside and oligonucleotide analogue |
| US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
| US6027998A (en) | 1997-12-17 | 2000-02-22 | Advanced Micro Devices, Inc. | Method for fully planarized conductive line for a stack gate |
| US6506594B1 (en) | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
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| WO2001092579A2 (en) | 2000-05-30 | 2001-12-06 | Pe Corporation (Ny) | Methods for detecting target nucleic acids using coupled ligation and amplification |
| US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US7294503B2 (en) | 2000-09-15 | 2007-11-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| US20100022414A1 (en) | 2008-07-18 | 2010-01-28 | Raindance Technologies, Inc. | Droplet Libraries |
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| CN102015522B (zh) | 2008-02-22 | 2013-07-03 | 弗卢迪格姆公司 | 用于微流体器件的集成载体 |
| US8697363B2 (en) | 2008-08-26 | 2014-04-15 | Fluidigm Corporation | Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags |
| EA020593B1 (ru) | 2009-01-13 | 2014-12-30 | Флуидигм Корпорейшн | Анализ нуклеиновых кислот, полученных из единичных клеток |
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| WO2014201273A1 (en) | 2013-06-12 | 2014-12-18 | The Broad Institute, Inc. | High-throughput rna-seq |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| CN107250445A (zh) | 2015-02-27 | 2017-10-13 | 富鲁达公司 | 用于高通量研究的单个细胞核酸 |
| US11117113B2 (en) | 2015-12-16 | 2021-09-14 | Fluidigm Corporation | High-level multiplex amplification |
-
2016
- 2016-02-26 CN CN201680012542.8A patent/CN107250445A/zh active Pending
- 2016-02-26 SG SG11201706636PA patent/SG11201706636PA/en unknown
- 2016-02-26 US US15/055,252 patent/US10190163B2/en active Active
- 2016-02-26 WO PCT/US2016/019952 patent/WO2016138490A1/en active Application Filing
- 2016-02-26 EP EP16756523.3A patent/EP3262214B1/de active Active
- 2016-02-26 JP JP2017542907A patent/JP2018509896A/ja active Pending
- 2016-02-26 CA CA2976681A patent/CA2976681A1/en active Pending
-
2018
- 2018-12-10 US US16/215,489 patent/US10954560B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3262214A1 (de) | 2018-01-03 |
| CN107250445A (zh) | 2017-10-13 |
| US10954560B2 (en) | 2021-03-23 |
| US20190185929A1 (en) | 2019-06-20 |
| US10190163B2 (en) | 2019-01-29 |
| WO2016138490A1 (en) | 2016-09-01 |
| EP3262214A4 (de) | 2018-07-25 |
| CA2976681A1 (en) | 2016-09-01 |
| EP3262214B1 (de) | 2023-10-25 |
| JP2018509896A (ja) | 2018-04-12 |
| US20160251714A1 (en) | 2016-09-01 |
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