SG11201607720XA - T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield - Google Patents

T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield

Info

Publication number
SG11201607720XA
SG11201607720XA SG11201607720XA SG11201607720XA SG11201607720XA SG 11201607720X A SG11201607720X A SG 11201607720XA SG 11201607720X A SG11201607720X A SG 11201607720XA SG 11201607720X A SG11201607720X A SG 11201607720XA SG 11201607720X A SG11201607720X A SG 11201607720XA
Authority
SG
Singapore
Prior art keywords
rna polymerase
substrate range
polymerase variants
expanded substrate
enhanced transcriptional
Prior art date
Application number
SG11201607720XA
Inventor
Andrew D Ellington
Adam J Meyer
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of SG11201607720XA publication Critical patent/SG11201607720XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
SG11201607720XA 2014-03-20 2015-03-20 T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield SG11201607720XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461968231P 2014-03-20 2014-03-20
PCT/US2015/021748 WO2015143318A1 (en) 2014-03-20 2015-03-20 T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield

Publications (1)

Publication Number Publication Date
SG11201607720XA true SG11201607720XA (en) 2016-10-28

Family

ID=54145383

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201607720XA SG11201607720XA (en) 2014-03-20 2015-03-20 T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield

Country Status (12)

Country Link
US (1) US9988612B2 (en)
EP (1) EP3119873A4 (en)
JP (1) JP2017509341A (en)
KR (1) KR20160132113A (en)
CN (1) CN106459879A (en)
AU (1) AU2015231031A1 (en)
BR (1) BR112016021627A2 (en)
CA (1) CA2942513A1 (en)
IL (1) IL247864A0 (en)
MX (1) MX2016012206A (en)
SG (1) SG11201607720XA (en)
WO (1) WO2015143318A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3645711A4 (en) 2017-06-30 2021-04-21 Codexis, Inc. T7 rna polymerase variants
KR20200023454A (en) 2017-06-30 2020-03-04 코덱시스, 인코포레이티드 T7 RNA Polymerase Variants
EP3668971B1 (en) * 2017-08-18 2024-04-10 ModernaTX, Inc. Rna polymerase variants
AU2019253693A1 (en) * 2018-04-10 2020-10-22 Greenlight Biosciences, Inc. T7 RNA polymerase variants
WO2021228905A2 (en) * 2020-05-11 2021-11-18 Thermo Fisher Scientific Baltics Uab Mutant polymerases and methods of using the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1261696B1 (en) * 2000-03-07 2005-05-04 bioMerieux B.V. Rna polymerase mutants with increased thermostability
JP2005346791A (en) 2004-06-01 2005-12-15 Tdk Corp Optical recording medium and its manufacturing method
JP5015923B2 (en) 2005-06-30 2012-09-05 アーケミックス コーポレイション Materials and methods for generating complete 2 'modified nucleic acid transcripts
WO2008078180A2 (en) * 2006-12-22 2008-07-03 Archemix Corp. Materials and methods for the generation of transcripts comprising modified nucleotides
EP2316934B1 (en) 2008-08-08 2015-05-13 Tosoh Corporation Rna polymerase mutant with improved functions
US9062292B2 (en) * 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
US9512456B2 (en) * 2012-08-14 2016-12-06 Modernatx, Inc. Enzymes and polymerases for the synthesis of RNA

Also Published As

Publication number Publication date
EP3119873A4 (en) 2018-01-03
JP2017509341A (en) 2017-04-06
BR112016021627A2 (en) 2018-07-10
IL247864A0 (en) 2016-11-30
KR20160132113A (en) 2016-11-16
WO2015143318A1 (en) 2015-09-24
US9988612B2 (en) 2018-06-05
CN106459879A (en) 2017-02-22
MX2016012206A (en) 2017-04-06
AU2015231031A1 (en) 2016-09-22
US20170211050A1 (en) 2017-07-27
CA2942513A1 (en) 2015-09-24
EP3119873A1 (en) 2017-01-25

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