SG11201607114VA - Expression constructs and methods for selecting host cells expressing polypeptides - Google Patents

Expression constructs and methods for selecting host cells expressing polypeptides

Info

Publication number
SG11201607114VA
SG11201607114VA SG11201607114VA SG11201607114VA SG11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA
Authority
SG
Singapore
Prior art keywords
methods
host cells
cells expressing
expression constructs
expressing polypeptides
Prior art date
Application number
SG11201607114VA
Inventor
Christel Aebischer-Gumy
Pierre Moretti
Martin Bertschinger
Original Assignee
Glenmark Pharmaceuticals Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glenmark Pharmaceuticals Sa filed Critical Glenmark Pharmaceuticals Sa
Publication of SG11201607114VA publication Critical patent/SG11201607114VA/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
SG11201607114VA 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides SG11201607114VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14157324 2014-02-28
PCT/EP2015/054331 WO2015128509A1 (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Publications (1)

Publication Number Publication Date
SG11201607114VA true SG11201607114VA (en) 2016-09-29

Family

ID=50184819

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201607114VA SG11201607114VA (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides
SG10201913396RA SG10201913396RA (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201913396RA SG10201913396RA (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Country Status (15)

Country Link
US (2) US20170350886A1 (en)
EP (1) EP3110950B1 (en)
JP (1) JP7008406B2 (en)
KR (1) KR20160135738A (en)
CN (1) CN106255752A (en)
AU (1) AU2015222065B2 (en)
BR (1) BR112016019832A2 (en)
CA (1) CA2940880C (en)
EA (1) EA035266B1 (en)
IL (1) IL247491A0 (en)
MA (1) MA39502A (en)
MX (1) MX2016011194A (en)
NZ (1) NZ723624A (en)
SG (2) SG11201607114VA (en)
WO (1) WO2015128509A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11623950B2 (en) * 2015-07-09 2023-04-11 Institut National De La Sante Et De La Recherche Medicale (Inserm) Lentiviral vector expressing membrane-anchored or secreted antibody
WO2017211900A1 (en) * 2016-06-07 2017-12-14 Max-Delbrück-Centrum für Molekulare Medizin Chimeric antigen receptor and car-t cells that bind bcma
MX2019012953A (en) * 2017-05-02 2020-02-12 Alligator Bioscience Ab Bispecific antibody against ox40 and ctla-4.
US20210363214A1 (en) * 2018-03-01 2021-11-25 University Of Washington Transmembrane polypeptides
KR20210025522A (en) * 2018-06-25 2021-03-09 유니버시티 오브 워싱톤 De novo design with powerful and selective interleukin mimics
AU2020304841A1 (en) * 2019-06-28 2021-12-23 F. Hoffmann-La Roche Ag Method for the production of an antibody
US11802866B2 (en) 2021-07-08 2023-10-31 Medic Life Sciences Inc. High-throughput screening system for identification of novel drugs and drug targets
CN113881701B (en) * 2021-09-08 2024-04-19 山东大学 Positive transfected cell affinity sorting method and kit

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4870208B2 (en) * 2006-05-17 2012-02-08 エフ．ホフマン−ラ ロシュ アーゲー Polypeptide-producing cells
US7947495B2 (en) * 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
US8969539B2 (en) * 2008-06-11 2015-03-03 Jn Biosciences Llc Expression vectors
WO2014126254A1 (en) * 2013-02-18 2014-08-21 協和発酵キリン株式会社 Membrane-binding protein expression vector

Also Published As

Publication number Publication date
SG10201913396RA (en) 2020-03-30
CN106255752A (en) 2016-12-21
EA035266B1 (en) 2020-05-22
KR20160135738A (en) 2016-11-28
BR112016019832A2 (en) 2017-10-17
EA201691548A1 (en) 2017-02-28
EP3110950A1 (en) 2017-01-04
WO2015128509A1 (en) 2015-09-03
AU2015222065B2 (en) 2021-05-20
JP2017508459A (en) 2017-03-30
CA2940880C (en) 2023-09-26
CA2940880A1 (en) 2015-09-03
US20170350886A1 (en) 2017-12-07
US20190056396A1 (en) 2019-02-21
EP3110950B1 (en) 2022-09-21
NZ723624A (en) 2022-07-29
MA39502A (en) 2015-09-03
JP7008406B2 (en) 2022-01-25
AU2015222065A1 (en) 2016-09-15
MX2016011194A (en) 2017-04-06
IL247491A0 (en) 2016-11-30

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