SG11201510297QA - Targeted integration - Google Patents

Targeted integration

Info

Publication number
SG11201510297QA
SG11201510297QA SG11201510297QA SG11201510297QA SG11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA
Authority
SG
Singapore
Prior art keywords
targeted integration
targeted
integration
Prior art date
Application number
SG11201510297QA
Inventor
Scott Bahr
Trissa Borgschulte
Kevin Kayser
Original Assignee
Sigma Aldrich Co Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201361837019P priority Critical
Application filed by Sigma Aldrich Co Llc filed Critical Sigma Aldrich Co Llc
Priority to PCT/US2014/043138 priority patent/WO2014205192A2/en
Publication of SG11201510297QA publication Critical patent/SG11201510297QA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301Endonuclease
SG11201510297QA 2013-06-19 2014-06-19 Targeted integration SG11201510297QA (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US201361837019P true 2013-06-19 2013-06-19
PCT/US2014/043138 WO2014205192A2 (en) 2013-06-19 2014-06-19 Targeted integration

Publications (1)

Publication Number Publication Date
SG11201510297QA true SG11201510297QA (en) 2016-01-28

Family

ID=52105507

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201510297QA SG11201510297QA (en) 2013-06-19 2014-06-19 Targeted integration

Country Status (12)

Country Link
US (1) US20160145645A1 (en)
EP (1) EP3011011A4 (en)
JP (1) JP2016523084A (en)
KR (1) KR20160021812A (en)
CN (1) CN105555948A (en)
AU (1) AU2014281472A1 (en)
BR (1) BR112015031639A2 (en)
CA (1) CA2915467A1 (en)
MX (1) MX2015017110A (en)
RU (1) RU2016101246A3 (en)
SG (1) SG11201510297QA (en)
WO (1) WO2014205192A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
JP6411463B2 (en) 2013-04-16 2018-10-24 リジェネロン・ファーマシューティカルズ・インコーポレイテッドRegeneron Pharmaceuticals, Inc. Targeted modification of rat genome
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9546384B2 (en) 2013-12-11 2017-01-17 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a mouse genome
US20150166980A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Fusions of cas9 domains and nucleic acid-editing domains
RU2704283C2 (en) 2014-06-06 2019-10-25 Регенерон Фармасьютикалз, Инк. Methods and compositions for modifying target locus
EP3177718A4 (en) 2014-07-30 2018-04-04 President and Fellows of Harvard College Cas9 proteins including ligand-dependent inteins
SG11201703747RA (en) 2014-11-21 2017-06-29 Regeneron Pharma METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAs
CN106554943A (en) * 2015-09-30 2017-04-05 北京吉尚立德生物科技有限公司 A kind of Chinese hamster ovary celI strain CHO-Creb3L1 of restructuring overexpression Creb3L1 genes
CN108699116A (en) 2015-10-23 2018-10-23 哈佛大学的校长及成员们 The CAS9 albumen of evolution for gene editing
WO2017201311A2 (en) * 2016-05-18 2017-11-23 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
US20180163218A1 (en) * 2016-12-14 2018-06-14 Dow Agrosciences Llc Reconstruction of site specific nuclease binding sites
EP3559236A1 (en) * 2016-12-20 2019-10-30 Development Center for Biotechnology Targeted integration sites in chinese hamster ovary cell genome
WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
GB201703416D0 (en) * 2017-03-03 2017-04-19 Ge Healthcare Bio-Sciences Ab Method for protein expression
GB201703418D0 (en) * 2017-03-03 2017-04-19 Ge Healthcare Bio-Sciences Ab Method for cell line development
AU2018315428A1 (en) * 2017-08-11 2020-01-16 Boehringer Ingelheim International Gmbh Integration sites in CHO cells
TW201932600A (en) * 2017-12-22 2019-08-16 美商建南德克公司 Targeted integration of nucleic acids

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1439234A1 (en) * 2003-01-08 2004-07-21 ARTEMIS Pharmaceuticals GmbH Targeted transgenesis using the rosa26 locus
WO2011104382A1 (en) * 2010-02-26 2011-09-01 Cellectis Use of endonucleases for inserting transgenes into safe harbor loci
US9567573B2 (en) * 2010-04-26 2017-02-14 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using nucleases
AU2012240164A1 (en) * 2011-04-05 2013-11-14 The Scripps Research Institute Chromosomal landing pads and related uses
CN103305504B (en) * 2012-03-14 2016-08-10 江苏吉锐生物技术有限公司 Compositions and the method for restructuring is pinpointed in hamster cell

Also Published As

Publication number Publication date
RU2016101246A3 (en) 2018-04-03
EP3011011A2 (en) 2016-04-27
KR20160021812A (en) 2016-02-26
MX2015017110A (en) 2016-08-03
AU2014281472A1 (en) 2016-01-21
JP2016523084A (en) 2016-08-08
RU2016101246A (en) 2017-07-24
CA2915467A1 (en) 2014-12-24
EP3011011A4 (en) 2017-05-31
BR112015031639A2 (en) 2019-09-03
US20160145645A1 (en) 2016-05-26
WO2014205192A3 (en) 2015-03-19
WO2014205192A2 (en) 2014-12-24
CN105555948A (en) 2016-05-04

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