SG11201510297QA - Targeted integration - Google Patents
Targeted integrationInfo
- Publication number
- SG11201510297QA SG11201510297QA SG11201510297QA SG11201510297QA SG11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA SG 11201510297Q A SG11201510297Q A SG 11201510297QA
- Authority
- SG
- Singapore
- Prior art keywords
- targeted integration
- targeted
- integration
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361837019P | 2013-06-19 | 2013-06-19 | |
| PCT/US2014/043138 WO2014205192A2 (en) | 2013-06-19 | 2014-06-19 | Targeted integration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201510297QA true SG11201510297QA (en) | 2016-01-28 |
Family
ID=52105507
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201510297QA SG11201510297QA (en) | 2013-06-19 | 2014-06-19 | Targeted integration |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20160145645A1 (en) |
| EP (1) | EP3011011A4 (en) |
| JP (1) | JP2016523084A (en) |
| KR (1) | KR20160021812A (en) |
| CN (1) | CN105555948A (en) |
| AU (1) | AU2014281472A1 (en) |
| BR (1) | BR112015031639A2 (en) |
| CA (1) | CA2915467A1 (en) |
| MX (1) | MX2015017110A (en) |
| RU (1) | RU2016101246A (en) |
| SG (1) | SG11201510297QA (en) |
| WO (1) | WO2014205192A2 (en) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| PT3456831T (en) | 2013-04-16 | 2021-09-10 | Regeneron Pharma | Targeted modification of rat genome |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9546384B2 (en) | 2013-12-11 | 2017-01-17 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a mouse genome |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| SG11201609634YA (en) | 2014-06-06 | 2016-12-29 | Regeneron Pharma | Methods and compositions for modifying a targeted locus |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| AU2015349692B2 (en) | 2014-11-21 | 2021-10-28 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide RNAs |
| CN106554943A (en) * | 2015-09-30 | 2017-04-05 | 北京吉尚立德生物科技有限公司 | A kind of Chinese hamster ovary celI strain CHO-Creb3L1 of restructuring overexpression Creb3L1 genes |
| US10167457B2 (en) | 2015-10-23 | 2019-01-01 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| US11505792B2 (en) * | 2016-04-11 | 2022-11-22 | Applied Stemcell, Inc. | Site-specific integration of transgenes |
| EP4219731A3 (en) * | 2016-05-18 | 2023-08-09 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nucleobase editors and uses thereof |
| US11078481B1 (en) | 2016-08-03 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for screening for cancer targets |
| CN109804066A (en) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | Programmable CAS9- recombination enzyme fusion proteins and application thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US11078483B1 (en) | 2016-09-02 | 2021-08-03 | KSQ Therapeutics, Inc. | Methods for measuring and improving CRISPR reagent function |
| AU2017342543A1 (en) | 2016-10-14 | 2019-05-02 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018111640A1 (en) * | 2016-12-14 | 2018-06-21 | Dow Agrosciences Llc | Reconstruction of site specific nuclease binding sites |
| EP3559236A4 (en) | 2016-12-20 | 2020-11-04 | Development Center for Biotechnology | Targeted integration sites in chinese hamster ovary cell genome |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| JP7048963B2 (en) * | 2016-12-28 | 2022-04-06 | 学校法人自治医科大学 | Gene expression control method and gene expression control kit |
| WO2018148196A1 (en) * | 2017-02-07 | 2018-08-16 | Sigma-Aldrich Co. Llc | Stable targeted integration |
| GB201703416D0 (en) * | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for protein expression |
| GB201703417D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
| GB201703418D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018175153A1 (en) * | 2017-03-19 | 2018-09-27 | Applied Stemcell, Inc. | Novel integration sites and uses thereof |
| JP7191388B2 (en) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| AU2018315428A1 (en) | 2017-08-11 | 2020-01-16 | Boehringer Ingelheim International Gmbh | Integration sites in CHO cells |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| EP3697906A1 (en) | 2017-10-16 | 2020-08-26 | The Broad Institute, Inc. | Uses of adenosine base editors |
| SG11202005109SA (en) * | 2017-12-22 | 2020-07-29 | Genentech Inc | Targeted integration of nucleic acids |
| CN110607326B (en) * | 2018-06-15 | 2022-11-29 | 江苏省农业科学院 | Non-strong start type exogenous gene expression method and application thereof in expression of target protein with toxicity |
| US11851663B2 (en) * | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| JP2022514342A (en) * | 2018-12-21 | 2022-02-10 | ジェネンテック， インコーポレイテッド | Targeted integration of nucleic acids |
| DE112020001342T5 (en) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Methods and compositions for editing nucleotide sequences |
| EP3956460A2 (en) * | 2019-04-18 | 2022-02-23 | Sigma-Aldrich Co. LLC | Stable targeted integration |
| MX2021015536A (en) * | 2019-06-19 | 2022-02-10 | Hoffmann La Roche | Method for the generation of a protein expressing cell by targeted integration using cre mrna. |
| EP3990649A1 (en) | 2019-06-26 | 2022-05-04 | Genentech, Inc. | Randomized configuration targeted integration of nucleic acids |
| JP2022541502A (en) * | 2019-07-18 | 2022-09-26 | ユニバーシティー オブ ロチェスター | Cell type-selective immunoprotection of cells |
| CN111088282B (en) * | 2020-03-23 | 2020-08-28 | 上海安民生物技术有限公司 | Application of AAVS1 and H11 safe harbor sites in recombinant expression protein |
| JP2023525304A (en) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| MX2022015205A (en) * | 2020-06-24 | 2023-01-05 | Genentech Inc | Targeted integration of nucleic acids. |
| WO2022104344A2 (en) * | 2020-11-10 | 2022-05-19 | The Board Of Trustees Of The Leland Stanford Junior University | Knock-in of large dna for long-term high genomic expression |
| CN112458059B (en) * | 2020-11-25 | 2021-07-23 | 杭州景杰生物科技股份有限公司 | Stable cell strain for recognizing H3K18la rabbit monoclonal antibody and construction method thereof |
| AU2022334794A1 (en) * | 2021-08-25 | 2024-03-28 | Iontas Limited | Preparation of libraries of protein variants expressed in eukaryotic cells |
| CN113881703B (en) * | 2021-10-11 | 2022-06-21 | 中国人民解放军军事科学院军事医学研究院 | Method for improving CHO cell homologous recombination efficiency and related product and application thereof |
| WO2023122246A1 (en) * | 2021-12-22 | 2023-06-29 | Genentech, Inc. | Multi-vector recombinase mediated cassette exchange |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1439234A1 (en) * | 2003-01-08 | 2004-07-21 | ARTEMIS Pharmaceuticals GmbH | Targeted transgenesis using the rosa26 locus |
| CN103097527A (en) * | 2010-02-26 | 2013-05-08 | 塞勒克提斯公司 | Use of endonucleases for inserting transgenes into safe harbor loci |
| CA2796600C (en) * | 2010-04-26 | 2019-08-13 | Sangamo Biosciences, Inc. | Genome editing of a rosa locus using zinc-finger nucleases |
| WO2012138887A1 (en) * | 2011-04-05 | 2012-10-11 | The Scripps Research Institute | Chromosomal landing pads and related uses |
| CN103305504B (en) * | 2012-03-14 | 2016-08-10 | 江苏吉锐生物技术有限公司 | Compositions and the method for restructuring is pinpointed in hamster cell |
-
2014
- 2014-06-19 JP JP2016521569A patent/JP2016523084A/en active Pending
- 2014-06-19 CN CN201480046042.7A patent/CN105555948A/en active Pending
- 2014-06-19 SG SG11201510297QA patent/SG11201510297QA/en unknown
- 2014-06-19 RU RU2016101246A patent/RU2016101246A/en not_active Application Discontinuation
- 2014-06-19 AU AU2014281472A patent/AU2014281472A1/en not_active Abandoned
- 2014-06-19 US US14/899,405 patent/US20160145645A1/en not_active Abandoned
- 2014-06-19 BR BR112015031639A patent/BR112015031639A2/en not_active Application Discontinuation
- 2014-06-19 MX MX2015017110A patent/MX2015017110A/en unknown
- 2014-06-19 EP EP14814484.3A patent/EP3011011A4/en not_active Withdrawn
- 2014-06-19 KR KR1020167000496A patent/KR20160021812A/en not_active Application Discontinuation
- 2014-06-19 WO PCT/US2014/043138 patent/WO2014205192A2/en active Application Filing
- 2014-06-19 CA CA2915467A patent/CA2915467A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20160021812A (en) | 2016-02-26 |
| RU2016101246A (en) | 2017-07-24 |
| BR112015031639A2 (en) | 2019-09-03 |
| RU2016101246A3 (en) | 2018-04-03 |
| US20160145645A1 (en) | 2016-05-26 |
| WO2014205192A2 (en) | 2014-12-24 |
| EP3011011A4 (en) | 2017-05-31 |
| JP2016523084A (en) | 2016-08-08 |
| CN105555948A (en) | 2016-05-04 |
| AU2014281472A1 (en) | 2016-01-21 |
| EP3011011A2 (en) | 2016-04-27 |
| CA2915467A1 (en) | 2014-12-24 |
| MX2015017110A (en) | 2016-08-03 |
| WO2014205192A3 (en) | 2015-03-19 |
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