SG11201509866TA - Protease-responsive peptide biosensors and methods for analyte detection - Google Patents

Protease-responsive peptide biosensors and methods for analyte detection

Info

Publication number
SG11201509866TA
SG11201509866TA SG11201509866TA SG11201509866TA SG11201509866TA SG 11201509866T A SG11201509866T A SG 11201509866TA SG 11201509866T A SG11201509866T A SG 11201509866TA SG 11201509866T A SG11201509866T A SG 11201509866TA SG 11201509866T A SG11201509866T A SG 11201509866TA
Authority
SG
Singapore
Prior art keywords
protease
methods
analyte detection
responsive peptide
peptide biosensors
Prior art date
Application number
SG11201509866TA
Inventor
Saurabh Rajendra Nirantar
Farid John Ghadessy
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Priority to SG11201509866TA priority Critical patent/SG11201509866TA/en
Publication of SG11201509866TA publication Critical patent/SG11201509866TA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845Methods of identifying protein-protein interactions in protein mixtures
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433Serine endopeptidases (3.4.21)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/02Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
SG11201509866TA 2013-06-06 2014-06-06 Protease-responsive peptide biosensors and methods for analyte detection SG11201509866TA (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SG11201509866TA SG11201509866TA (en) 2013-06-06 2014-06-06 Protease-responsive peptide biosensors and methods for analyte detection

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SG2013043955 2013-06-06
SG11201509866TA SG11201509866TA (en) 2013-06-06 2014-06-06 Protease-responsive peptide biosensors and methods for analyte detection
PCT/SG2014/000263 WO2014196932A1 (en) 2013-06-06 2014-06-06 Protease-responsive peptide biosensors and methods for analyte detection

Publications (1)

Publication Number Publication Date
SG11201509866TA true SG11201509866TA (en) 2015-12-30

Family

ID=52008440

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201509866TA SG11201509866TA (en) 2013-06-06 2014-06-06 Protease-responsive peptide biosensors and methods for analyte detection

Country Status (4)

Country Link
US (1) US20160109438A1 (en)
EP (1) EP3004375A4 (en)
SG (1) SG11201509866TA (en)
WO (1) WO2014196932A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY196175A (en) * 2016-01-11 2023-03-20 Univ Leland Stanford Junior Chimeric Proteins And Methods Of Regulating Gene Expression
MY194127A (en) 2016-01-11 2022-11-14 Univ Leland Stanford Junior Chimeric proteins and methods of immunotherapy
US20190360019A1 (en) * 2016-11-16 2019-11-28 Universal Biosensors Pty Ltd Cleavage event transduction methods and products
SG11202000145XA (en) * 2017-07-12 2020-02-27 Refuge Biotechnologies Inc Methods and systems for conditionally regulating gene expression
US20210396762A1 (en) * 2020-06-19 2021-12-23 Encodia, Inc. Methods for peptide analysis employing multi-component detection agent and related kits

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884870B2 (en) * 1998-03-20 2005-04-26 California Institute Of Technology Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
NO20053354D0 (en) * 2005-07-11 2005-07-11 Amersham Health As Optical imaging contrast agent.
WO2007127538A1 (en) * 2006-03-16 2007-11-08 Invitrogen Corporation Methods for assaying protein-protein interaction
US20100278745A1 (en) * 2006-12-21 2010-11-04 Norbert Lange Compounds for fluorescence imaging
PT2191275E (en) * 2007-09-04 2012-11-16 Sanofi Sa Identifying molecules that modulate protein- protein interactions using protease activated reporters

Also Published As

Publication number Publication date
WO2014196932A1 (en) 2014-12-11
EP3004375A4 (en) 2017-05-10
EP3004375A1 (en) 2016-04-13
US20160109438A1 (en) 2016-04-21

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