SG11201508732PA - Method for refining protein including self-cutting cassette and use thereof - Google Patents
Method for refining protein including self-cutting cassette and use thereofInfo
- Publication number
- SG11201508732PA SG11201508732PA SG11201508732PA SG11201508732PA SG11201508732PA SG 11201508732P A SG11201508732P A SG 11201508732PA SG 11201508732P A SG11201508732P A SG 11201508732PA SG 11201508732P A SG11201508732P A SG 11201508732PA SG 11201508732P A SG11201508732P A SG 11201508732PA
- Authority
- SG
- Singapore
- Prior art keywords
- protein including
- including self
- cutting cassette
- refining protein
- refining
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Oncology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Analytical Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20130046322 | 2013-04-25 | ||
| PCT/KR2014/003639 WO2014175690A1 (en) | 2013-04-25 | 2014-04-25 | Method for refining protein including self-cutting cassette and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201508732PA true SG11201508732PA (en) | 2015-11-27 |
Family
ID=51792155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201508732PA SG11201508732PA (en) | 2013-04-25 | 2014-04-25 | Method for refining protein including self-cutting cassette and use thereof |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US10077299B2 (en) |
| EP (1) | EP2990423B1 (en) |
| JP (1) | JP6176392B2 (en) |
| KR (1) | KR101808223B1 (en) |
| CN (1) | CN105339391B (en) |
| AU (1) | AU2014258109B2 (en) |
| CA (1) | CA2909513C (en) |
| IL (1) | IL242147B (en) |
| NZ (1) | NZ713602A (en) |
| RU (1) | RU2639527C2 (en) |
| SG (1) | SG11201508732PA (en) |
| WO (1) | WO2014175690A1 (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2777714A1 (en) | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
| CN108026560A (en) | 2015-09-25 | 2018-05-11 | 豪夫迈·罗氏有限公司 | Reacted in eutectic solvent using the acid amides that turns of sorting enzyme |
| CN108138204B (en) | 2015-09-25 | 2021-12-31 | 豪夫迈·罗氏有限公司 | Method for producing thioester using sortase A |
| WO2017050866A1 (en) | 2015-09-25 | 2017-03-30 | F. Hoffmann-La Roche Ag | Novel soluble sortase a |
| JP6727325B2 (en) * | 2016-03-30 | 2020-07-22 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Improved sortase |
| US10738338B2 (en) | 2016-10-18 | 2020-08-11 | The Research Foundation for the State University | Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate |
| MX2020011844A (en) * | 2018-05-08 | 2021-04-13 | Amgen Inc | Bispecific antibodies with cleavable c-terminal charge-paired tags. |
| US20220010032A1 (en) * | 2018-11-07 | 2022-01-13 | Kawasaki Institute Of Industrial Promotion | Peptide-nucleic acid complex |
| CN110357970A (en) * | 2019-07-10 | 2019-10-22 | 杭州纽龙日尚生物制品有限公司 | A kind of hEGF's fusion protein and its nucleic acid molecules, a kind of hEGF's preparation method for being easy to purify |
| KR20240043837A (en) * | 2022-09-27 | 2024-04-04 | 크리포 주식회사 | A fusion protein that forms self-assembly including purification tag and alpha-helix tag, and method for purifying recombinant protein using the same |
| ES2980877A1 (en) * | 2023-03-03 | 2024-10-03 | Univ Santiago Compostela | muNS FUSION PROTEIN CAPABLE OF FORMING MICROSPHERES AND ITS USES |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT410798B (en) * | 2001-01-26 | 2003-07-25 | Cistem Biotechnologies Gmbh | METHOD FOR IDENTIFYING, ISOLATING AND PRODUCING ANTIGENS AGAINST A SPECIFIC PATHOGEN |
| US20070148166A1 (en) * | 2002-05-13 | 2007-06-28 | Wu Madeline C S | Scfvs in photosynthetic microbes |
| WO2005051976A2 (en) | 2003-11-20 | 2005-06-09 | Ansata Therapeutics, Inc. | Protein and peptide ligation processes and one-step purification processes |
| WO2007108013A2 (en) | 2006-03-22 | 2007-09-27 | National Institute Of Immunology | Novel bioconjugates as therapeutic agent and synthesis thereof |
| JP2009055837A (en) * | 2007-08-31 | 2009-03-19 | Shionogi & Co Ltd | Method for immobilizing protein |
| WO2010087994A2 (en) | 2009-01-30 | 2010-08-05 | Whitehead Institute For Biomedical Research | Methods for ligation and uses thereof |
| WO2011056911A2 (en) * | 2009-11-04 | 2011-05-12 | Alnylam Pharmaceuticals, Inc. | Compositions and methods for enhancing production of a biological product |
| AU2012275390A1 (en) | 2011-06-28 | 2014-01-16 | Whitehead Institute For Biomedical Research | Using sortases to install click chemistry handles for protein ligation |
| EP2949789B1 (en) | 2013-01-24 | 2021-07-28 | AbTLAS Co., Ltd. | Protein combination-based fv library, and preparation method therefor |
| US9631218B2 (en) | 2013-03-15 | 2017-04-25 | The Trustees Of The University Of Pennsylvania | Sortase-mediated protein purification and ligation |
| EP2777714A1 (en) * | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
-
2014
- 2014-04-25 CN CN201480033267.9A patent/CN105339391B/en active Active
- 2014-04-25 EP EP14787873.0A patent/EP2990423B1/en active Active
- 2014-04-25 SG SG11201508732PA patent/SG11201508732PA/en unknown
- 2014-04-25 WO PCT/KR2014/003639 patent/WO2014175690A1/en active Application Filing
- 2014-04-25 NZ NZ713602A patent/NZ713602A/en not_active IP Right Cessation
- 2014-04-25 RU RU2015150335A patent/RU2639527C2/en not_active IP Right Cessation
- 2014-04-25 US US14/785,881 patent/US10077299B2/en active Active
- 2014-04-25 AU AU2014258109A patent/AU2014258109B2/en not_active Ceased
- 2014-04-25 JP JP2016510621A patent/JP6176392B2/en active Active
- 2014-04-25 KR KR1020157030691A patent/KR101808223B1/en active IP Right Grant
- 2014-04-25 CA CA2909513A patent/CA2909513C/en not_active Expired - Fee Related
-
2015
- 2015-10-18 IL IL242147A patent/IL242147B/en not_active IP Right Cessation
-
2018
- 2018-08-09 US US16/100,080 patent/US20190077846A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| RU2639527C2 (en) | 2017-12-21 |
| AU2014258109B2 (en) | 2016-09-29 |
| EP2990423A4 (en) | 2016-10-26 |
| CN105339391A (en) | 2016-02-17 |
| NZ713602A (en) | 2016-09-30 |
| RU2015150335A (en) | 2017-05-31 |
| US10077299B2 (en) | 2018-09-18 |
| IL242147B (en) | 2019-03-31 |
| KR20160007509A (en) | 2016-01-20 |
| CA2909513C (en) | 2018-10-09 |
| US20160137720A1 (en) | 2016-05-19 |
| EP2990423B1 (en) | 2018-04-18 |
| CA2909513A1 (en) | 2014-10-30 |
| KR101808223B1 (en) | 2017-12-13 |
| WO2014175690A1 (en) | 2014-10-30 |
| JP2016519118A (en) | 2016-06-30 |
| AU2014258109A1 (en) | 2015-11-12 |
| CN105339391B (en) | 2021-01-01 |
| EP2990423A1 (en) | 2016-03-02 |
| JP6176392B2 (en) | 2017-08-09 |
| US20190077846A1 (en) | 2019-03-14 |
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