SG11201505941PA - Cytoplasmic expression of fab proteins - Google Patents

Cytoplasmic expression of fab proteins

Info

Publication number
SG11201505941PA
SG11201505941PA SG11201505941PA SG11201505941PA SG11201505941PA SG 11201505941P A SG11201505941P A SG 11201505941PA SG 11201505941P A SG11201505941P A SG 11201505941PA SG 11201505941P A SG11201505941P A SG 11201505941PA SG 11201505941P A SG11201505941P A SG 11201505941PA
Authority
SG
Singapore
Prior art keywords
cytoplasmic expression
fab proteins
fab
proteins
cytoplasmic
Prior art date
Application number
SG11201505941PA
Inventor
Matthew Beasley
Benjamin Kiefel
Keith Niven
Original Assignee
Affinity Biosciences Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2013900572A external-priority patent/AU2013900572A0/en
Application filed by Affinity Biosciences Pty Ltd filed Critical Affinity Biosciences Pty Ltd
Publication of SG11201505941PA publication Critical patent/SG11201505941PA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/70Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088Compounds having three or more nucleosides or nucleotides
    • A61K31/711Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • C07K2317/522CH1 domain
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565Complementarity determining region [CDR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/80Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C07K2317/82Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies functional in the cytoplasm, the inner aspect of the cell membrane, the nucleus or the mitochondria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/04Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
SG11201505941PA 2013-02-20 2014-02-20 Cytoplasmic expression of fab proteins SG11201505941PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2013900572A AU2013900572A0 (en) 2013-02-20 Cytoplasmic expression of fab proteins
PCT/IB2014/059107 WO2014128628A1 (en) 2013-02-20 2014-02-20 Cytoplasmic expression of fab proteins

Publications (1)

Publication Number Publication Date
SG11201505941PA true SG11201505941PA (en) 2015-09-29

Family

ID=51390573

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201505941PA SG11201505941PA (en) 2013-02-20 2014-02-20 Cytoplasmic expression of fab proteins

Country Status (6)

Country Link
US (1) US20160002317A1 (en)
EP (1) EP2959041A4 (en)
AU (1) AU2014220306A1 (en)
CA (1) CA2901385A1 (en)
SG (1) SG11201505941PA (en)
WO (1) WO2014128628A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6244301B2 (en) 2011-08-18 2017-12-06 アフィニティ バイオサイエンス ピーティーワイ リミテッド Soluble polypeptide
GB2616707B (en) * 2023-01-10 2024-04-17 Rxbiologics Ltd Antibody discovery methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2331570B1 (en) * 2008-09-10 2014-08-27 Philochem AG Display library for antibody selection
US8716196B2 (en) * 2009-05-20 2014-05-06 Novimmune S.A. Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants
NZ596603A (en) * 2009-05-29 2013-07-26 Morphosys Ag A collection of antibodies or fragments having properties relevant to developability
US10093920B2 (en) * 2009-12-23 2018-10-09 Affinity Biosciences Pty Ltd Protein display
SG195215A1 (en) * 2011-06-29 2013-12-30 Affinity Biosciences Pty Ltd Method of protein display
JP6244301B2 (en) * 2011-08-18 2017-12-06 アフィニティ バイオサイエンス ピーティーワイ リミテッド Soluble polypeptide

Also Published As

Publication number Publication date
EP2959041A1 (en) 2015-12-30
EP2959041A4 (en) 2016-08-24
AU2014220306A1 (en) 2015-08-27
WO2014128628A1 (en) 2014-08-28
CA2901385A1 (en) 2014-08-28
US20160002317A1 (en) 2016-01-07

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