SG11201408028YA - METHOD FOR PURIFICATION OF RECOMBINANT FACTOR Xa DERIVATIVES - Google Patents

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 19 December 2013 (19.12.2013) WIPOIPCT (10) International Publication Number WO 2013/188587 A1 (51) International Patent Classification: C07K1/22 (2006.01) C12N 9/64 (2006.01) (21) International Application Number: PCT/US2013/045496 (22) International Filing Date: 12 June 2013 (12.06.2013) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 61/659,821 14 June 2012 (14.06.2012) US (71) Applicant: PORTOLA PHARMACEUTICALS, INC. [US/US]; 270 E. Grand Avenue, South San Francisco, California 94080 (US). (72) Inventors: LU, Genmin; 1439 El Camino Real, Apt 9, Burlingame, California 94010 (US). SINHA, Uma; 808 Junipero Serra Blvd., San Francisco, California 94127 (US). (74) Agents: TANNER, Lorna L. et al.; Foley & Lardner LLP, 3000 K Street N.W., Suite 600, Washington, DC 20007- 5109 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: — with international search report (Art. 21(3)) (54) Title: METHOD FOR PURIFICATION OF RECOMBINANT FACTOR Xa DERIVATIVES l> 00 00 00 T-H rn T-H o CJ o - A280 ELISA CM < 0 0- O O -O- O- O O < /: \ t \ // \ -*-V / \ / \ —£ - O O—O 0 0 0 0 A 0 80000 60000 - E 40000 < to 20000 4 6 Fraction # 10 12 r-Antidote MK r-Antidote (57) Abstract: Disclosed herein are methods and kits for purifying a serine protease. The methods entail loading the serine protease to a soybean trypsin inhibitor (STI) based affinity chromatograph, and eluting the serine protease with an elution buffer comprising an agent that disrupts interaction between the STI and the serine protease