SG10202110989QA - Large scale pei-mediated plasmid transfection - Google Patents
Large scale pei-mediated plasmid transfectionInfo
- Publication number
- SG10202110989QA SG10202110989QA SG10202110989QA SG10202110989QA SG10202110989QA SG 10202110989Q A SG10202110989Q A SG 10202110989QA SG 10202110989Q A SG10202110989Q A SG 10202110989QA SG 10202110989Q A SG10202110989Q A SG 10202110989QA SG 10202110989Q A SG10202110989Q A SG 10202110989QA
- Authority
- SG
- Singapore
- Prior art keywords
- large scale
- plasmid transfection
- mediated plasmid
- pei
- scale pei
- Prior art date
Links
- 230000001404 mediated effect Effects 0.000 title 1
- 239000013612 plasmid Substances 0.000 title 1
- 238000001890 transfection Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/18—Fixed or packed bed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15051—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Clinical Laboratory Science (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2017/025681 WO2017180341A2 (en) | 2016-04-14 | 2017-04-03 | Fixed-bed bioreactor with constant-flow pump/ tubing system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10202110989QA true SG10202110989QA (en) | 2021-11-29 |
Family
ID=66522911
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10202110989QA SG10202110989QA (en) | 2017-04-03 | 2017-04-25 | Large scale pei-mediated plasmid transfection |
| SG11201808700QA SG11201808700QA (en) | 2016-04-14 | 2017-04-25 | Large scale pei-mediated plasmid transfection |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201808700QA SG11201808700QA (en) | 2016-04-14 | 2017-04-25 | Large scale pei-mediated plasmid transfection |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US11261416B2 (en) |
| EP (1) | EP3497224B1 (en) |
| KR (3) | KR20230022177A (en) |
| CN (2) | CN116144500A (en) |
| CA (1) | CA3021045A1 (en) |
| ES (1) | ES2951678T3 (en) |
| FI (1) | FI3497224T3 (en) |
| SG (2) | SG10202110989QA (en) |
| WO (1) | WO2018007873A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230022177A (en) | 2016-04-14 | 2023-02-14 | 트리젤 엘티디. | Fixed-bed bioreactor with constant-flow pumpi tubing system |
| EP3722436A1 (en) | 2016-04-14 | 2020-10-14 | Trizell Ltd. | Fixed-bed bioreactor with constant-flow pump/tubing system |
| BE1026108B1 (en) * | 2018-03-16 | 2019-10-14 | Univercells S.A. | FIXED BED SAMPLE AND ASSOCIATED METHODS |
| EP4146810A1 (en) | 2020-05-04 | 2023-03-15 | AGC Biologics S.p.A. | Lentiviral vector manufacturing process in packed bed bioreactor |
| CN112779226A (en) * | 2021-02-03 | 2021-05-11 | 苏州博腾生物制药有限公司 | Culture process of lentivirus adherent cells |
| CN114317395B (en) * | 2021-12-31 | 2024-04-02 | 中国肉类食品综合研究中心 | Large-scale culture process for adherent cells |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63313576A (en) | 1987-06-13 | 1988-12-21 | Rikagaku Kenkyusho | Tubular bio-reactor |
| US5622857A (en) | 1995-08-08 | 1997-04-22 | Genespan Corporation | High performance cell culture bioreactor and method |
| AU732703B2 (en) * | 1996-11-20 | 2001-04-26 | Crucell Holland B.V. | An improved method for the production and purification of adenoviral vectors |
| IL163800A0 (en) | 2002-03-12 | 2005-12-18 | Ark Therapeutics Ltd | Engineered baculoviruses and their use |
| CN1930281A (en) | 2004-03-05 | 2007-03-14 | 帝斯曼知识产权资产管理有限公司 | Process for cell culturing by continuous perfusion and alternating tangential flow |
| US20110189764A1 (en) * | 2011-03-27 | 2011-08-04 | Nathan Starbard | Single use device incorporating a container and scaffold |
| CN105143443A (en) | 2013-02-22 | 2015-12-09 | 建新公司 | Microcarrier perfusion culturing methods and uses thereof |
| MX2015012114A (en) * | 2013-03-08 | 2016-01-12 | Genzyme Corp | Continuous purification of therapeutic proteins. |
| GB201417042D0 (en) * | 2014-09-29 | 2014-11-12 | Fkd Therapies Oy | Method |
| US20160257972A1 (en) * | 2015-03-04 | 2016-09-08 | Children's Hospital Medical Center | Foamy viral vector compositions and methods for the manufacture of same |
| KR20230022177A (en) | 2016-04-14 | 2023-02-14 | 트리젤 엘티디. | Fixed-bed bioreactor with constant-flow pumpi tubing system |
| EP3722436A1 (en) | 2016-04-14 | 2020-10-14 | Trizell Ltd. | Fixed-bed bioreactor with constant-flow pump/tubing system |
-
2017
- 2017-04-03 KR KR1020227044118A patent/KR20230022177A/en not_active Application Discontinuation
- 2017-04-03 US US15/579,208 patent/US11261416B2/en active Active
- 2017-04-03 CN CN202310104669.4A patent/CN116144500A/en active Pending
- 2017-04-25 EP EP17791136.9A patent/EP3497224B1/en active Active
- 2017-04-25 CA CA3021045A patent/CA3021045A1/en active Pending
- 2017-04-25 CN CN201780023080.4A patent/CN109072252A/en active Pending
- 2017-04-25 WO PCT/IB2017/001205 patent/WO2018007873A1/en active Application Filing
- 2017-04-25 SG SG10202110989QA patent/SG10202110989QA/en unknown
- 2017-04-25 KR KR1020247003513A patent/KR20240025689A/en not_active Application Discontinuation
- 2017-04-25 KR KR1020187032283A patent/KR20190039021A/en not_active IP Right Cessation
- 2017-04-25 FI FIEP17791136.9T patent/FI3497224T3/en active
- 2017-04-25 ES ES17791136T patent/ES2951678T3/en active Active
- 2017-04-25 SG SG11201808700QA patent/SG11201808700QA/en unknown
-
2019
- 2019-03-19 US US16/357,532 patent/US11242504B2/en active Active
-
2021
- 2021-12-22 US US17/559,366 patent/US20220364036A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20190039021A (en) | 2019-04-10 |
| KR20240025689A (en) | 2024-02-27 |
| US20190218495A1 (en) | 2019-07-18 |
| WO2018007873A1 (en) | 2018-01-11 |
| CN109072252A (en) | 2018-12-21 |
| FI3497224T3 (en) | 2023-08-17 |
| ES2951678T3 (en) | 2023-10-24 |
| SG11201808700QA (en) | 2018-11-29 |
| CA3021045A1 (en) | 2018-01-11 |
| EP3497224A1 (en) | 2019-06-19 |
| WO2018007873A4 (en) | 2018-03-01 |
| US11242504B2 (en) | 2022-02-08 |
| EP3497224B1 (en) | 2023-06-07 |
| US20190031998A1 (en) | 2019-01-31 |
| US20220364036A1 (en) | 2022-11-17 |
| KR20230022177A (en) | 2023-02-14 |
| US11261416B2 (en) | 2022-03-01 |
| CN116144500A (en) | 2023-05-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL268894A (en) | Therapeutic rna | |
| ZA201608209B (en) | Stable cannabinoid formulations | |
| IL248229A (en) | Drug related transgene expression | |
| TWI561137B (en) | Curved display | |
| SG10202110989QA (en) | Large scale pei-mediated plasmid transfection | |
| IL273595A (en) | Modified cpf1 guide rna | |
| PL3112825T3 (en) | Combination weighing apparatus | |
| IL254486A0 (en) | Weighing take-off | |
| SG11201704450UA (en) | Coexpression plasmid | |
| IL270933A (en) | Targeted therapeutics | |
| HK1232184A1 (en) | Batch mode soil improvement | |
| PL3442345T3 (en) | Offset weighing apparatus | |
| GB201612976D0 (en) | Non conducting material | |
| SG11201702516UA (en) | Novel selection marker for cell transfection and protein production | |
| IL280640A (en) | Nanoparticles for transfection | |
| AU363102S (en) | Gauges | |
| IL270932A (en) | Targeted therapeutics | |
| GB201805865D0 (en) | Genes | |
| GB2544498B (en) | Macerators | |
| GB2568088B (en) | Cultivation arrangement | |
| GB201514944D0 (en) | Scales | |
| GB2534255B (en) | Pogo stick | |
| GB201707913D0 (en) | Protective material | |
| GB201720948D0 (en) | Plasmid system | |
| GB201903873D0 (en) | Enhanced transfection |