SG10201903626XA - Light-activated two-component protein binding matrix - Google Patents

Light-activated two-component protein binding matrix

Info

Publication number
SG10201903626XA
SG10201903626XA SG10201903626XA SG10201903626XA SG10201903626XA SG 10201903626X A SG10201903626X A SG 10201903626XA SG 10201903626X A SG10201903626X A SG 10201903626XA SG 10201903626X A SG10201903626X A SG 10201903626XA SG 10201903626X A SG10201903626X A SG 10201903626XA
Authority
SG
Singapore
Prior art keywords
light
binding
activated
pair
component protein
Prior art date
Application number
SG10201903626XA
Inventor
Chester Lee Drum
Original Assignee
Nat Univ Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Univ Singapore filed Critical Nat Univ Singapore
Publication of SG10201903626XA publication Critical patent/SG10201903626XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/12—Radicals substituted by oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/10—Spiro-condensed systems
    • C07D491/107—Spiro-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G01N33/547—Synthetic resin with antigen or antibody attached to the carrier via a bridging agent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Indole Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

LIGHT-ACTIVATED TWO-COMPONENT PROTEIN BINDING MATRIX A binding pair for chromatographic separation or purification of a molecule of interest, where one binding member of the pair is an isomerizable organic molecule and the other binding member of the pair is an isomer-specific affinity agent bound to a molecule of interest. The binding pair associates and disassociates upon exposure to a binding agent, such as using light, decreased intensity of light, darkness, heat, stress, ions, an isomerizable affinity agent, change in pH, or a combination thereof. (FIG. 1C)
SG10201903626XA 2014-10-30 2015-10-29 Light-activated two-component protein binding matrix SG10201903626XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462072727P 2014-10-30 2014-10-30

Publications (1)

Publication Number Publication Date
SG10201903626XA true SG10201903626XA (en) 2019-05-30

Family

ID=55857951

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201903626XA SG10201903626XA (en) 2014-10-30 2015-10-29 Light-activated two-component protein binding matrix
SG11201702843XA SG11201702843XA (en) 2014-10-30 2015-10-29 Light-activated two-component protein binding matrix

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201702843XA SG11201702843XA (en) 2014-10-30 2015-10-29 Light-activated two-component protein binding matrix

Country Status (5)

Country Link
US (1) US20170349546A1 (en)
EP (1) EP3224260A4 (en)
JP (2) JP2018507164A (en)
SG (2) SG10201903626XA (en)
WO (1) WO2016068809A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090280060A1 (en) * 2004-11-18 2009-11-12 Marriott Gerard J D Photochromic probes

Also Published As

Publication number Publication date
SG11201702843XA (en) 2017-05-30
JP2021006594A (en) 2021-01-21
EP3224260A4 (en) 2018-08-01
WO2016068809A1 (en) 2016-05-06
EP3224260A1 (en) 2017-10-04
JP2018507164A (en) 2018-03-15
US20170349546A1 (en) 2017-12-07
JP6846728B2 (en) 2021-03-24

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