SG10201803344PA - Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution - Google Patents

Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution

Info

Publication number
SG10201803344PA
SG10201803344PA SG10201803344PA SG10201803344PA SG10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA SG 10201803344P A SG10201803344P A SG 10201803344PA
Authority
SG
Singapore
Prior art keywords
fluorophore
chain reaction
polymerase chain
dna
quencher probes
Prior art date
Application number
SG10201803344PA
Inventor
Jackie Y Ying
Saravana Kumar Kumarasamy
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG10201803344PA publication Critical patent/SG10201803344PA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Abstract

NONMOTORIZED OPTICAL MULTIPLEXING FOR THE SIMULTANEOUS DETECTION OF DNA TARGET AMPLICONS IN A POLYMERASE CHAIN REACTION SOLUTION A multiplexed polymerase chain reaction (PCR) DNA detection system and a method for DNA detection within the PCR system are provided. The PCR DNA detection system includes a color charge-coupled device (CCD) camera, fluorophore- quencher probes and an imaging chamber. The fluorophore-quencher probes are selected in response to fluorophores quenched by the fluorophore-quencher probes corresponding to three selected primary colors and peak channel responses of the CCD camera such that emission profiles of the fluorophores substantially match the three selected primary colors and peak emission profiles of the fluorophores correspond to the peak RGB channel responses of the CCD camera. A DNA sample and the fluorophore-quencher probes are located within the imaging chamber. The color CCD camera is focused on the imaging chamber for simultaneous detection of up to three targets from fluorescence of the DNA sample and the fluorophore- quencher probes. FIG. . -30-
SG10201803344PA 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution SG10201803344PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SG2013076500 2013-10-14

Publications (1)

Publication Number Publication Date
SG10201803344PA true SG10201803344PA (en) 2018-06-28

Family

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Family Applications (2)

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SG11201602873XA SG11201602873XA (en) 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
SG10201803344PA SG10201803344PA (en) 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201602873XA SG11201602873XA (en) 2013-10-14 2014-10-14 Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution

Country Status (4)

Country Link
US (1) US10718012B2 (en)
CN (1) CN105960466B (en)
SG (2) SG11201602873XA (en)
WO (1) WO2015057165A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201608301UA (en) 2014-04-09 2016-11-29 Agency Science Tech & Res Microfluidic device
RU2702577C2 (en) * 2015-02-06 2019-10-08 Лайф Текнолоджиз Корпорейшн Systems and methods for biological analysis
EP3639014A4 (en) * 2017-06-14 2020-12-30 Ubiqd Inc. Fiber-coupled broadband light source
SG10201801853WA (en) * 2018-03-02 2019-10-30 Delta Electronics Int’L Singapore Pte Ltd Portable multi-color fluorescence detection device
CN109085118A (en) * 2018-06-28 2018-12-25 陈思 A kind of novel miniaturization POCT detection device
US20230296513A1 (en) * 2020-07-23 2023-09-21 Life Technologies Corporation Systems and methods for biological analysis

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US20040191786A1 (en) * 2001-02-16 2004-09-30 Yue David T. Three cube fret method (3-fret) for detecting fluorescence energy transfer
US8137616B2 (en) * 2003-04-04 2012-03-20 Roche Molecular Systems, Inc. System for multi color real time PCR
US7432055B2 (en) * 2004-03-05 2008-10-07 Uchicago Argonne Llc Dual phase multiplex polymerase chain reaction
EP2021773B1 (en) * 2006-04-19 2015-03-25 IT-IS International Ltd Reaction monitoring method and apparatus
EP2225600A2 (en) * 2007-09-05 2010-09-08 Chroma Technology Corporation Light source
US8722017B2 (en) * 2008-07-10 2014-05-13 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent magnetic nanoprobes, methods of making, and methods of use
DK2584344T3 (en) * 2009-01-08 2021-10-04 It Is International Ltd Optical system for detecting light from polymerase chain reactions
JP2012529908A (en) * 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド Improved method for quantification of forensic DNA
US8997838B2 (en) * 2009-07-10 2015-04-07 Keihin Corporation Vehicular air conditioning apparatus
US8104605B2 (en) * 2009-07-14 2012-01-31 Jansson Claes E Material sample collector
JP5978220B2 (en) * 2010-10-29 2016-08-24 プレジデント アンド フェローズ オブ ハーバード カレッジ Nucleic acid nanostructure barcode probe
EP2658998B1 (en) * 2010-12-29 2015-04-22 Life Technologies Corporation Ddao compounds as fluorescent reference standards
JP2016509206A (en) * 2012-12-21 2016-03-24 マイクロニクス， インコーポレイテッド Portable fluorescence detection system and microassay cartridge

Also Published As

Publication number Publication date
SG11201602873XA (en) 2016-05-30
US10718012B2 (en) 2020-07-21
CN105960466B (en) 2019-12-31
CN105960466A (en) 2016-09-21
WO2015057165A1 (en) 2015-04-23
US20160230213A1 (en) 2016-08-11

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