SE9901306D0 - Improved TIRF chamber - Google Patents
Improved TIRF chamberInfo
- Publication number
- SE9901306D0 SE9901306D0 SE9901306A SE9901306A SE9901306D0 SE 9901306 D0 SE9901306 D0 SE 9901306D0 SE 9901306 A SE9901306 A SE 9901306A SE 9901306 A SE9901306 A SE 9901306A SE 9901306 D0 SE9901306 D0 SE 9901306D0
- Authority
- SE
- Sweden
- Prior art keywords
- chamber
- catchers
- sample
- fluorescent groups
- tirf
- Prior art date
Links
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A biochemical sample chamber (6) is made of a transparent material. At least two of the inner chamber walls (2, 3) act as substrates and are provided with catchers (5), such as chemical or affinity reagents. In use, the catchers are coupled to fluorescent groups (4) within the sample in a pattern representative for the sample. The fluorescent groups (4) of each chamber wall (2, 3) are excited, using total internal reflection technique, by light introduced to prisms (7, 8) corresponding to each inner wall which has been provided with catchers. The excited fluorescent groups emit light that is detected. Thus, the inner space of the chamber is used with high efficiency, thereby enabling an analizing capacity which is least twice that of a single chamber.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9901306A SE9901306D0 (en) | 1999-04-09 | 1999-04-09 | Improved TIRF chamber |
PCT/EP2000/003185 WO2000062042A1 (en) | 1999-04-09 | 2000-04-07 | Sample cuvette for measurements of total internal reflection fluorescence |
EP00920681A EP1169633A1 (en) | 1999-04-09 | 2000-04-07 | Sample cuvette for measurements of total internal reflection fluorescence |
AU41169/00A AU4116900A (en) | 1999-04-09 | 2000-04-07 | Sample cuvette for measurements of total internal reflection fluorescence |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9901306A SE9901306D0 (en) | 1999-04-09 | 1999-04-09 | Improved TIRF chamber |
Publications (1)
Publication Number | Publication Date |
---|---|
SE9901306D0 true SE9901306D0 (en) | 1999-04-09 |
Family
ID=20415189
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE9901306A SE9901306D0 (en) | 1999-04-09 | 1999-04-09 | Improved TIRF chamber |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1169633A1 (en) |
AU (1) | AU4116900A (en) |
SE (1) | SE9901306D0 (en) |
WO (1) | WO2000062042A1 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EE04249B1 (en) * | 1999-04-21 | 2004-02-16 | Asper O� | Method for reading biopolymer matrix and fluorescence detector |
SE9902474D0 (en) | 1999-06-30 | 1999-06-30 | Amersham Pharm Biotech Ab | Polymer valves |
SE0004296D0 (en) | 2000-11-23 | 2000-11-23 | Gyros Ab | Device and method for the controlled heating in micro channel systems |
US7429354B2 (en) | 2001-03-19 | 2008-09-30 | Gyros Patent Ab | Structural units that define fluidic functions |
CA2441206A1 (en) | 2001-03-19 | 2002-09-26 | Gyros Ab | Characterization of reaction variables |
US6919058B2 (en) | 2001-08-28 | 2005-07-19 | Gyros Ab | Retaining microfluidic microcavity and other microfluidic structures |
EP2283924B1 (en) | 2001-08-28 | 2013-04-17 | Gyros Patent Ab | Inlet unit with means supporting liquid entrance into a microchannel structure |
US7189368B2 (en) | 2001-09-17 | 2007-03-13 | Gyros Patent Ab | Functional unit enabling controlled flow in a microfluidic device |
JP4519124B2 (en) | 2003-01-30 | 2010-08-04 | ユィロス・パテント・アクチボラグ | Wall inside the microfluidic device |
SE0300823D0 (en) | 2003-03-23 | 2003-03-23 | Gyros Ab | Preloaded Microscale Devices |
JP2007502428A (en) | 2003-05-23 | 2007-02-08 | ユィロス・パテント・アクチボラグ | Fluid function based on non-wetting surface |
SE0400181D0 (en) | 2004-01-29 | 2004-01-29 | Gyros Ab | Segmented porous and preloaded microscale devices |
DE102004039564B4 (en) * | 2004-08-13 | 2006-06-29 | Institut für Lasertechnologien in der Medizin und Meßtechnik an der Universität Ulm | Apparatus for optically screening biological sample surfaces, e.g. in drug screening or diagnostic tests, comprises exciting fluorescence simultaneously in multiple samples with evanescent electromagnetic field using split beam |
US9993819B2 (en) | 2014-12-30 | 2018-06-12 | Stmicroelectronics S.R.L. | Apparatus for actuating and reading a centrifugal microfluidic disk for biological and biochemical analyses, and use of the apparatus |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3481644D1 (en) * | 1984-12-10 | 1990-04-19 | Prutec Ltd | METHOD FOR THE OPTICAL DETECTION OF PARAMETERS OF SUBSTANCES IN A LIQUID ANALYT. |
CA1341592C (en) * | 1989-07-07 | 2009-04-14 | Abbott Laboratories | Ion capture reagents and methods for performing binding assays |
ATE377751T1 (en) * | 1995-05-12 | 2007-11-15 | Novartis Erfind Verwalt Gmbh | METHOD FOR THE PARALLEL DETERMINATION OF SEVERAL ANALYTES USING EVANESCENTLY EXCITED LUMINESCENCE |
-
1999
- 1999-04-09 SE SE9901306A patent/SE9901306D0/en unknown
-
2000
- 2000-04-07 WO PCT/EP2000/003185 patent/WO2000062042A1/en active Search and Examination
- 2000-04-07 EP EP00920681A patent/EP1169633A1/en not_active Withdrawn
- 2000-04-07 AU AU41169/00A patent/AU4116900A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2000062042A1 (en) | 2000-10-19 |
AU4116900A (en) | 2000-11-14 |
EP1169633A1 (en) | 2002-01-09 |
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