SE9901306D0 - Improved TIRF chamber - Google Patents

Improved TIRF chamber

Info

Publication number
SE9901306D0
SE9901306D0 SE9901306A SE9901306A SE9901306D0 SE 9901306 D0 SE9901306 D0 SE 9901306D0 SE 9901306 A SE9901306 A SE 9901306A SE 9901306 A SE9901306 A SE 9901306A SE 9901306 D0 SE9901306 D0 SE 9901306D0
Authority
SE
Sweden
Prior art keywords
chamber
catchers
sample
fluorescent groups
tirf
Prior art date
Application number
SE9901306A
Other languages
Swedish (sv)
Inventor
Per Johan Ulfendahl
Original Assignee
Amersham Pharm Biotech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharm Biotech Ab filed Critical Amersham Pharm Biotech Ab
Priority to SE9901306A priority Critical patent/SE9901306D0/en
Publication of SE9901306D0 publication Critical patent/SE9901306D0/en
Priority to PCT/EP2000/003185 priority patent/WO2000062042A1/en
Priority to EP00920681A priority patent/EP1169633A1/en
Priority to AU41169/00A priority patent/AU4116900A/en

Links

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A biochemical sample chamber (6) is made of a transparent material. At least two of the inner chamber walls (2, 3) act as substrates and are provided with catchers (5), such as chemical or affinity reagents. In use, the catchers are coupled to fluorescent groups (4) within the sample in a pattern representative for the sample. The fluorescent groups (4) of each chamber wall (2, 3) are excited, using total internal reflection technique, by light introduced to prisms (7, 8) corresponding to each inner wall which has been provided with catchers. The excited fluorescent groups emit light that is detected. Thus, the inner space of the chamber is used with high efficiency, thereby enabling an analizing capacity which is least twice that of a single chamber.
SE9901306A 1999-04-09 1999-04-09 Improved TIRF chamber SE9901306D0 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
SE9901306A SE9901306D0 (en) 1999-04-09 1999-04-09 Improved TIRF chamber
PCT/EP2000/003185 WO2000062042A1 (en) 1999-04-09 2000-04-07 Sample cuvette for measurements of total internal reflection fluorescence
EP00920681A EP1169633A1 (en) 1999-04-09 2000-04-07 Sample cuvette for measurements of total internal reflection fluorescence
AU41169/00A AU4116900A (en) 1999-04-09 2000-04-07 Sample cuvette for measurements of total internal reflection fluorescence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9901306A SE9901306D0 (en) 1999-04-09 1999-04-09 Improved TIRF chamber

Publications (1)

Publication Number Publication Date
SE9901306D0 true SE9901306D0 (en) 1999-04-09

Family

ID=20415189

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9901306A SE9901306D0 (en) 1999-04-09 1999-04-09 Improved TIRF chamber

Country Status (4)

Country Link
EP (1) EP1169633A1 (en)
AU (1) AU4116900A (en)
SE (1) SE9901306D0 (en)
WO (1) WO2000062042A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EE04249B1 (en) * 1999-04-21 2004-02-16 Asper O� Method for reading biopolymer matrix and fluorescence detector
SE9902474D0 (en) 1999-06-30 1999-06-30 Amersham Pharm Biotech Ab Polymer valves
SE0004296D0 (en) 2000-11-23 2000-11-23 Gyros Ab Device and method for the controlled heating in micro channel systems
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
CA2441206A1 (en) 2001-03-19 2002-09-26 Gyros Ab Characterization of reaction variables
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
EP2283924B1 (en) 2001-08-28 2013-04-17 Gyros Patent Ab Inlet unit with means supporting liquid entrance into a microchannel structure
US7189368B2 (en) 2001-09-17 2007-03-13 Gyros Patent Ab Functional unit enabling controlled flow in a microfluidic device
JP4519124B2 (en) 2003-01-30 2010-08-04 ユィロス・パテント・アクチボラグ Wall inside the microfluidic device
SE0300823D0 (en) 2003-03-23 2003-03-23 Gyros Ab Preloaded Microscale Devices
JP2007502428A (en) 2003-05-23 2007-02-08 ユィロス・パテント・アクチボラグ Fluid function based on non-wetting surface
SE0400181D0 (en) 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
DE102004039564B4 (en) * 2004-08-13 2006-06-29 Institut für Lasertechnologien in der Medizin und Meßtechnik an der Universität Ulm Apparatus for optically screening biological sample surfaces, e.g. in drug screening or diagnostic tests, comprises exciting fluorescence simultaneously in multiple samples with evanescent electromagnetic field using split beam
US9993819B2 (en) 2014-12-30 2018-06-12 Stmicroelectronics S.R.L. Apparatus for actuating and reading a centrifugal microfluidic disk for biological and biochemical analyses, and use of the apparatus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3481644D1 (en) * 1984-12-10 1990-04-19 Prutec Ltd METHOD FOR THE OPTICAL DETECTION OF PARAMETERS OF SUBSTANCES IN A LIQUID ANALYT.
CA1341592C (en) * 1989-07-07 2009-04-14 Abbott Laboratories Ion capture reagents and methods for performing binding assays
ATE377751T1 (en) * 1995-05-12 2007-11-15 Novartis Erfind Verwalt Gmbh METHOD FOR THE PARALLEL DETERMINATION OF SEVERAL ANALYTES USING EVANESCENTLY EXCITED LUMINESCENCE

Also Published As

Publication number Publication date
WO2000062042A1 (en) 2000-10-19
AU4116900A (en) 2000-11-14
EP1169633A1 (en) 2002-01-09

Similar Documents

Publication Publication Date Title
SE9901306D0 (en) Improved TIRF chamber
ATE363652T1 (en) FLUOROMETRIC DETECTION WITH VISIBLE LIGHT
TW326073B (en) Capillary array electrophoresis system
US9719925B2 (en) Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
DE69720763D1 (en) SETS OF, WITH FLUORESCENT ENERGY TRANSFER CONNECTIONS, MARKED PRIMERS AND THEIR USE IN MULTI-COMPONENT ANALYSIS
SE0203781D0 (en) Holder and method for cooling or heating samples
ES2035068T3 (en) REAGENT KIT TO BE USED WHEN CALIBRATING AN INSTRUMENT FOR PERFORMING ANALYSIS OF PARTICULATE BICROMATIC FLUORESCENCE.
EA199700219A1 (en) MULTIMATRIC MULTISPECIFIC ELECTROCHEMILUMINESCENT TEST
TR200001647T2 (en) High efficiency scanning in continuous format
WO2002035260A3 (en) Light detection device
CA2006222A1 (en) Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
DK1032705T3 (en) Probe arrays and methods for using probe arrays to distinguish DNA
WO1999024797A8 (en) Single molecule identification using selected fluorescence characteristics
ATE383661T1 (en) HIGH PERFORMANCE LED LAMP SYSTEM
AU7709198A (en) Low background multi-well plates for fluorescence measurements of biological andbiochemical samples
CY1110438T1 (en) CHEMICAL (Fusion Proteins) GFP-AQUORINE AS BIOSLIGHTERS REFERENCES CA ++ ON A LEVEL OF A CELL
WO2003027648A3 (en) Compact multiwavelength phase fluorometer
WO2003027741A3 (en) Biochemical assay detection in a liquid receptacle using a fiber optic exciter
ES2081991T3 (en) DETECTION OR QUANTIFICATION OF SEVERAL ANALYZES USING MARKING TECHNIQUES.
BR9912825A (en) Device for detecting fluorescent species
BR0309231A (en) Thermally Conductive Biological Test Tray, and Method for Manufacturing
ES2189844T3 (en) SUBSTITUTED 1,2-DIOXETHANE COMPOUNDS THAT PRESENT A SOLUBILITY IN INCREASED WATER AND TEST COMPOSITIONS.
DK0898557T3 (en) Water-soluble trisubstituted 1,2-dioxetane, which has improved storage stability, synthetic processes and intermediates
MXPA01013031A (en) Method for determining the volume of single red blood cells.
DE50307388D1 (en) PROCESS FOR SUBMITTING SAMPLING CHAMBERS WITH SUSPENSION SAMPLES