SE9801029D0 - New process - Google Patents

New process

Info

Publication number
SE9801029D0
SE9801029D0 SE9801029A SE9801029A SE9801029D0 SE 9801029 D0 SE9801029 D0 SE 9801029D0 SE 9801029 A SE9801029 A SE 9801029A SE 9801029 A SE9801029 A SE 9801029A SE 9801029 D0 SE9801029 D0 SE 9801029D0
Authority
SE
Sweden
Prior art keywords
buffy coat
leukocytes
coat fraction
blood
achieve
Prior art date
Application number
SE9801029A
Other languages
Swedish (sv)
Other versions
SE9801029L (en
SE521954C2 (en
Inventor
Mats Jarekrans
Original Assignee
Bionative Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionative Ab filed Critical Bionative Ab
Priority to SE9801029A priority Critical patent/SE521954C2/en
Publication of SE9801029D0 publication Critical patent/SE9801029D0/en
Priority to PCT/SE1999/000452 priority patent/WO1999049016A1/en
Priority to EP99916097A priority patent/EP1078041B1/en
Priority to US09/646,985 priority patent/US6743624B1/en
Priority to DK99916097T priority patent/DK1078041T3/en
Priority to HU0103429A priority patent/HU228495B1/en
Priority to DE69920209T priority patent/DE69920209T2/en
Priority to AT99916097T priority patent/ATE276356T1/en
Priority to AU34470/99A priority patent/AU3447099A/en
Priority to ES99916097T priority patent/ES2224631T3/en
Publication of SE9801029L publication Critical patent/SE9801029L/en
Priority to HK01105898A priority patent/HK1035210A1/en
Publication of SE521954C2 publication Critical patent/SE521954C2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4612—B-cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • External Artificial Organs (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method and apparatus for continuous purification and concentration of leukocytes from blood are provided. Continuous purification and concentration of leukocytes from blood comprises: (a) separating plasma from the blood by filtration to achieve a filtered buffy coat fraction; (b) adding an aqueous solution which is hypotonic in relation to plasma, to the buffy coat fraction from step (a) to achieve lysation of erythrocytes contained in buffy coat fraction; (c) mixing the buffy coat fraction and the aqueous hypotonic solution in a mixing device; (d) leading the mixture from (c) through a retention vessel; (e) leading the mixture from (d) through a centrifuge to separate the leukocytes; and (f) collecting the separated leukocytes from (e). An Independent claim is also included for the apparatus for continuous purification and concentration of leukocytes from blood comprising: (i) a membrane filter means for separating plasma from the blood by filtration to achieve a filtered buffy coat fraction; (ii) a static mixer for mixing the buffy coat fraction and an aqueous hypotonic solution to achieve lysation of erythrocytes contained in the buffy coat fraction; (iii) a retention vessel means; and (iv) a centrifuge means for separating leukocytes.
SE9801029A 1998-03-26 1998-03-26 Continuous purification and concentration of leukocytes from blood, useful for production of interferons SE521954C2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
SE9801029A SE521954C2 (en) 1998-03-26 1998-03-26 Continuous purification and concentration of leukocytes from blood, useful for production of interferons
ES99916097T ES2224631T3 (en) 1998-03-26 1999-03-23 PROCESS FOR CONTINUOUS PURIFICATION AND CONCENTRATION OF LEUKOCYTES FROM BLOOD.
DK99916097T DK1078041T3 (en) 1998-03-26 1999-03-23 Method for Continuous Purification and Concentration of Blood Leukocytes
EP99916097A EP1078041B1 (en) 1998-03-26 1999-03-23 Process for continuous purification and concentration of leukocytes from blood
US09/646,985 US6743624B1 (en) 1998-03-26 1999-03-23 Process for continuous purification and concentration of leukocytes from blood
PCT/SE1999/000452 WO1999049016A1 (en) 1998-03-26 1999-03-23 Process for continuous purification and concentration of leukocytes from blood
HU0103429A HU228495B1 (en) 1998-03-26 1999-03-23 Process for continuous purification and concentration of leukocytes from blood
DE69920209T DE69920209T2 (en) 1998-03-26 1999-03-23 METHOD FOR CONTINUOUS PURIFICATION AND CONCENTRATION OF LEUKOCYTES FROM THE BLOOD
AT99916097T ATE276356T1 (en) 1998-03-26 1999-03-23 METHOD FOR THE CONTINUOUS PURIFICATION AND CONCENTRATION OF LEUKOCYTES FROM BLOOD
AU34470/99A AU3447099A (en) 1998-03-26 1999-03-23 Process for continuous purification and concentration of leukocytes from blood
HK01105898A HK1035210A1 (en) 1998-03-26 2001-08-21 Process for continuous purification and concentration of leukocytes from blood.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9801029A SE521954C2 (en) 1998-03-26 1998-03-26 Continuous purification and concentration of leukocytes from blood, useful for production of interferons

Publications (3)

Publication Number Publication Date
SE9801029D0 true SE9801029D0 (en) 1998-03-26
SE9801029L SE9801029L (en) 1999-11-26
SE521954C2 SE521954C2 (en) 2003-12-23

Family

ID=20410715

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9801029A SE521954C2 (en) 1998-03-26 1998-03-26 Continuous purification and concentration of leukocytes from blood, useful for production of interferons

Country Status (1)

Country Link
SE (1) SE521954C2 (en)

Also Published As

Publication number Publication date
SE9801029L (en) 1999-11-26
SE521954C2 (en) 2003-12-23

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Legal Events

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