SE9500361D0 - Method and apparatus for detecting fluoroformed substances - Google Patents

Method and apparatus for detecting fluoroformed substances

Info

Publication number
SE9500361D0
SE9500361D0 SE9500361A SE9500361A SE9500361D0 SE 9500361 D0 SE9500361 D0 SE 9500361D0 SE 9500361 A SE9500361 A SE 9500361A SE 9500361 A SE9500361 A SE 9500361A SE 9500361 D0 SE9500361 D0 SE 9500361D0
Authority
SE
Sweden
Prior art keywords
fluorophore
substances
detecting
fluoroformed
wavelength
Prior art date
Application number
SE9500361A
Other languages
Swedish (sv)
Inventor
Bengt Goeran Andersson
Original Assignee
Pharmacia Biotech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech Ab filed Critical Pharmacia Biotech Ab
Priority to SE9500361A priority Critical patent/SE9500361D0/en
Publication of SE9500361D0 publication Critical patent/SE9500361D0/en
Priority to PCT/SE1996/000109 priority patent/WO1996024042A1/en
Priority to EP96902044A priority patent/EP0807247A1/en
Priority to JP8523474A priority patent/JPH10513555A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

In a method and an apparatus for detecting substances which are labelled with a fluorophore having a light absorption maximum at a wavelength between 480 and 520 nm by excitation of the fluorophore by means of light from a laser (1), and detection of light emitted by the fluorophore by means of a photodetector (7), either an Nd:YAG-laser emitting at a wavelength of 473 nm or a frequency-doubled laser diode emitting at a wavelength of 490 nm, is selected for the excitation of the fluorophore.
SE9500361A 1995-02-01 1995-02-01 Method and apparatus for detecting fluoroformed substances SE9500361D0 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
SE9500361A SE9500361D0 (en) 1995-02-01 1995-02-01 Method and apparatus for detecting fluoroformed substances
PCT/SE1996/000109 WO1996024042A1 (en) 1995-02-01 1996-01-31 Method and apparatus for detecting fluorophore labelled substances
EP96902044A EP0807247A1 (en) 1995-02-01 1996-01-31 Method and apparatus for detecting fluorophore labelled substances
JP8523474A JPH10513555A (en) 1995-02-01 1996-01-31 Method and apparatus for detecting a phosphor label

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9500361A SE9500361D0 (en) 1995-02-01 1995-02-01 Method and apparatus for detecting fluoroformed substances

Publications (1)

Publication Number Publication Date
SE9500361D0 true SE9500361D0 (en) 1995-02-01

Family

ID=20397051

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9500361A SE9500361D0 (en) 1995-02-01 1995-02-01 Method and apparatus for detecting fluoroformed substances

Country Status (4)

Country Link
EP (1) EP0807247A1 (en)
JP (1) JPH10513555A (en)
SE (1) SE9500361D0 (en)
WO (1) WO1996024042A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0814594B1 (en) * 1996-06-18 2004-09-08 Fuji Photo Film Co., Ltd. Image reading apparatus
JP4812393B2 (en) 2005-03-04 2011-11-09 株式会社日立ハイテクノロジーズ Fluorescent molecule measurement system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5207880A (en) * 1984-03-29 1993-05-04 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4675300A (en) * 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation

Also Published As

Publication number Publication date
WO1996024042A1 (en) 1996-08-08
EP0807247A1 (en) 1997-11-19
JPH10513555A (en) 1998-12-22

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