SE9301583D0 - YEAST STRAIN AND METHODS FOR EXPRESSING HETEROLOGOUS PROTEINS IN YEAST - Google Patents
YEAST STRAIN AND METHODS FOR EXPRESSING HETEROLOGOUS PROTEINS IN YEASTInfo
- Publication number
- SE9301583D0 SE9301583D0 SE19939301583A SE9301583A SE9301583D0 SE 9301583 D0 SE9301583 D0 SE 9301583D0 SE 19939301583 A SE19939301583 A SE 19939301583A SE 9301583 A SE9301583 A SE 9301583A SE 9301583 D0 SE9301583 D0 SE 9301583D0
- Authority
- SE
- Sweden
- Prior art keywords
- yeast
- strain
- methods
- heterologous proteins
- protein
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract 1
- 238000005621 mannosylation reaction Methods 0.000 abstract 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Botany (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Saccharomyces cerevisiae strain, characterized in that it is defective in O-mannosylation of a hydroxyl group of at least one serine or at least one threonine residue in a protein expressed by the strain. In the preferred strains the protein is heterologous and encoded in a vector. Process for the production of a protein that normally is O-glycosylated by S. cerevisiae using the strain to express the protein.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE19939301583A SE9301583D0 (en) | 1993-05-07 | 1993-05-07 | YEAST STRAIN AND METHODS FOR EXPRESSING HETEROLOGOUS PROTEINS IN YEAST |
JP6525318A JPH08509867A (en) | 1993-05-07 | 1994-05-06 | Methods for expressing heterologous structural proteins in yeast strains and yeast. |
PCT/SE1994/000421 WO1994026873A1 (en) | 1993-05-07 | 1994-05-06 | Yeast strain and methods for expressing heterologous proteins in yeast |
CA002161973A CA2161973A1 (en) | 1993-05-07 | 1994-05-06 | Yeast strain and methods for expressing heterologous proteins in yeast |
EP94915327A EP0698086A1 (en) | 1993-05-07 | 1994-05-06 | Yeast strain and methods for expressing heterologous proteins in yeast |
AU66626/94A AU683061B2 (en) | 1993-05-07 | 1994-05-06 | Yeast strain and methods for expressing heterologous proteins in yeast |
NZ266045A NZ266045A (en) | 1993-05-07 | 1994-05-06 | Saccharomyces cerevisiae strain defective in o-mannosylation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE19939301583A SE9301583D0 (en) | 1993-05-07 | 1993-05-07 | YEAST STRAIN AND METHODS FOR EXPRESSING HETEROLOGOUS PROTEINS IN YEAST |
Publications (1)
Publication Number | Publication Date |
---|---|
SE9301583D0 true SE9301583D0 (en) | 1993-05-07 |
Family
ID=20389870
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE19939301583A SE9301583D0 (en) | 1993-05-07 | 1993-05-07 | YEAST STRAIN AND METHODS FOR EXPRESSING HETEROLOGOUS PROTEINS IN YEAST |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0698086A1 (en) |
JP (1) | JPH08509867A (en) |
AU (1) | AU683061B2 (en) |
CA (1) | CA2161973A1 (en) |
NZ (1) | NZ266045A (en) |
SE (1) | SE9301583D0 (en) |
WO (1) | WO1994026873A1 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5728553A (en) | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
GB9902000D0 (en) * | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
AU2002227311A1 (en) * | 2000-12-05 | 2002-06-18 | The Penn State Research Foundation | Methods and compositions for highly efficient production of heterologous proteins in yeast |
DK1789442T3 (en) * | 2004-09-02 | 2009-11-16 | Lilly Co Eli | Muteins of fibroblast growth factor 21 |
ES2553162T3 (en) * | 2006-05-16 | 2015-12-04 | Daiichi Sankyo Company, Limited | High protein secretion production method |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0276846A3 (en) * | 1987-01-29 | 1989-07-26 | Zymogenetics, Inc. | Colony-stimulating factor derivatives |
AU625392B2 (en) * | 1987-10-29 | 1992-07-09 | Zymogenetics Inc. | Methods of regulating protein glycosylation |
-
1993
- 1993-05-07 SE SE19939301583A patent/SE9301583D0/en unknown
-
1994
- 1994-05-06 CA CA002161973A patent/CA2161973A1/en not_active Abandoned
- 1994-05-06 NZ NZ266045A patent/NZ266045A/en unknown
- 1994-05-06 WO PCT/SE1994/000421 patent/WO1994026873A1/en not_active Application Discontinuation
- 1994-05-06 EP EP94915327A patent/EP0698086A1/en not_active Withdrawn
- 1994-05-06 AU AU66626/94A patent/AU683061B2/en not_active Ceased
- 1994-05-06 JP JP6525318A patent/JPH08509867A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
NZ266045A (en) | 1997-06-24 |
EP0698086A1 (en) | 1996-02-28 |
CA2161973A1 (en) | 1994-11-24 |
AU683061B2 (en) | 1997-10-30 |
WO1994026873A1 (en) | 1994-11-24 |
JPH08509867A (en) | 1996-10-22 |
AU6662694A (en) | 1994-12-12 |
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