SE8903865D0 - EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS - Google Patents

EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS

Info

Publication number
SE8903865D0
SE8903865D0 SE8903865A SE8903865A SE8903865D0 SE 8903865 D0 SE8903865 D0 SE 8903865D0 SE 8903865 A SE8903865 A SE 8903865A SE 8903865 A SE8903865 A SE 8903865A SE 8903865 D0 SE8903865 D0 SE 8903865D0
Authority
SE
Sweden
Prior art keywords
expression
dna fragment
molecule
host cell
introducing
Prior art date
Application number
SE8903865A
Other languages
Swedish (sv)
Inventor
L Hansson
Original Assignee
Kabigen Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabigen Ab filed Critical Kabigen Ab
Priority to SE8903865A priority Critical patent/SE8903865D0/en
Publication of SE8903865D0 publication Critical patent/SE8903865D0/en
Priority to PCT/SE1990/000747 priority patent/WO1991007497A1/en

Links

Abstract

A process for the production of a heterologous polypeptide or protein molecule through expression in a eucaryotic host cell, comprising the steps: (a) transforming said host cell by introducing therein an expression vector, said vector comprising an upstream regulatory sequence, a first DNA fragment encoding said molecule and positioned downstream of said regulatory sequence, which is capable of initiating RNA transcription of said DNA fragment, and further comprising a second DNA fragment with signals for efficient RNA processing located downstream of said first DNA fragment; (b) culturing the transformed host cell in a suitable medium under conditions allowing expression of the desired molecule; and (c) recovering the molecule resulting from step (b) from the culture; host cells transformed by introducing therein such an expression vector; and polypeptide and protein molecules prepared by such process.
SE8903865A 1989-11-17 1989-11-17 EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS SE8903865D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE8903865A SE8903865D0 (en) 1989-11-17 1989-11-17 EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS
PCT/SE1990/000747 WO1991007497A1 (en) 1989-11-17 1990-11-19 Expression of heterologous proteins using eucaryotic cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8903865A SE8903865D0 (en) 1989-11-17 1989-11-17 EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS

Publications (1)

Publication Number Publication Date
SE8903865D0 true SE8903865D0 (en) 1989-11-17

Family

ID=20377505

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8903865A SE8903865D0 (en) 1989-11-17 1989-11-17 EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS

Country Status (2)

Country Link
SE (1) SE8903865D0 (en)
WO (1) WO1991007497A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0625574A1 (en) * 1993-05-19 1994-11-23 Takeda Chemical Industries, Ltd. Production for biologically active polypeptide
WO1995016772A1 (en) * 1993-12-14 1995-06-22 Cornell Research Foundation, Inc. Adenovirus gene expression system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0255320A3 (en) * 1986-07-28 1989-09-06 Genzyme Corporation Production of proteins in myeloma cells
DE3730599A1 (en) * 1986-09-12 1988-07-07 Genentech Inc METHOD FOR THE CONTINUOUS PRODUCTION OF HETEROLOGICAL PROTEINS IN EUKARYONTIC HOST CELLS

Also Published As

Publication number Publication date
WO1991007497A1 (en) 1991-05-30

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