SE8903865D0 - EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS - Google Patents
EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLSInfo
- Publication number
- SE8903865D0 SE8903865D0 SE8903865A SE8903865A SE8903865D0 SE 8903865 D0 SE8903865 D0 SE 8903865D0 SE 8903865 A SE8903865 A SE 8903865A SE 8903865 A SE8903865 A SE 8903865A SE 8903865 D0 SE8903865 D0 SE 8903865D0
- Authority
- SE
- Sweden
- Prior art keywords
- expression
- dna fragment
- molecule
- host cell
- introducing
- Prior art date
Links
Abstract
A process for the production of a heterologous polypeptide or protein molecule through expression in a eucaryotic host cell, comprising the steps: (a) transforming said host cell by introducing therein an expression vector, said vector comprising an upstream regulatory sequence, a first DNA fragment encoding said molecule and positioned downstream of said regulatory sequence, which is capable of initiating RNA transcription of said DNA fragment, and further comprising a second DNA fragment with signals for efficient RNA processing located downstream of said first DNA fragment; (b) culturing the transformed host cell in a suitable medium under conditions allowing expression of the desired molecule; and (c) recovering the molecule resulting from step (b) from the culture; host cells transformed by introducing therein such an expression vector; and polypeptide and protein molecules prepared by such process.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8903865A SE8903865D0 (en) | 1989-11-17 | 1989-11-17 | EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS |
PCT/SE1990/000747 WO1991007497A1 (en) | 1989-11-17 | 1990-11-19 | Expression of heterologous proteins using eucaryotic cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8903865A SE8903865D0 (en) | 1989-11-17 | 1989-11-17 | EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS |
Publications (1)
Publication Number | Publication Date |
---|---|
SE8903865D0 true SE8903865D0 (en) | 1989-11-17 |
Family
ID=20377505
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8903865A SE8903865D0 (en) | 1989-11-17 | 1989-11-17 | EXPRESSION OF HETEROLOGOUS PROTEINS USING EUKARYOTIC CELLS |
Country Status (2)
Country | Link |
---|---|
SE (1) | SE8903865D0 (en) |
WO (1) | WO1991007497A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0625574A1 (en) * | 1993-05-19 | 1994-11-23 | Takeda Chemical Industries, Ltd. | Production for biologically active polypeptide |
WO1995016772A1 (en) * | 1993-12-14 | 1995-06-22 | Cornell Research Foundation, Inc. | Adenovirus gene expression system |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0255320A3 (en) * | 1986-07-28 | 1989-09-06 | Genzyme Corporation | Production of proteins in myeloma cells |
DE3730599A1 (en) * | 1986-09-12 | 1988-07-07 | Genentech Inc | METHOD FOR THE CONTINUOUS PRODUCTION OF HETEROLOGICAL PROTEINS IN EUKARYONTIC HOST CELLS |
-
1989
- 1989-11-17 SE SE8903865A patent/SE8903865D0/en unknown
-
1990
- 1990-11-19 WO PCT/SE1990/000747 patent/WO1991007497A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO1991007497A1 (en) | 1991-05-30 |
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