SE8800676L - DETERMINATION OF REACTANTS AND COMBINATIONS OF REACTANTS INVOLVED IN BIOSPECIFIC REACTIONS - Google Patents

DETERMINATION OF REACTANTS AND COMBINATIONS OF REACTANTS INVOLVED IN BIOSPECIFIC REACTIONS

Info

Publication number
SE8800676L
SE8800676L SE8800676A SE8800676A SE8800676L SE 8800676 L SE8800676 L SE 8800676L SE 8800676 A SE8800676 A SE 8800676A SE 8800676 A SE8800676 A SE 8800676A SE 8800676 L SE8800676 L SE 8800676L
Authority
SE
Sweden
Prior art keywords
adsorption
thio
ether
complex
reactants
Prior art date
Application number
SE8800676A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE8800676D0 (en
SE462165B (en
Inventor
J Porath
S Oscarsson
Original Assignee
Porath Jerker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Porath Jerker filed Critical Porath Jerker
Priority to SE8800676A priority Critical patent/SE462165B/en
Publication of SE8800676D0 publication Critical patent/SE8800676D0/en
Priority to PCT/SE1989/000078 priority patent/WO1989008257A1/en
Publication of SE8800676L publication Critical patent/SE8800676L/en
Publication of SE462165B publication Critical patent/SE462165B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3253—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure not containing any of the heteroatoms nitrogen, oxygen or sulfur, e.g. aromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3255—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

A desired immunoglobulin, enzyme inhibitor, lectin, nucleic acid or nucleic acid derivative is separated from a protein complex through adsorption on a polymer carrier such as a polyhydric organic polymer, silicon gel, organic polyamine or polyamide or the like, into which have been introduced ligands containing an aliphatic group consisting of a thio-ether and a sulphone group or a thio-ether and an aromatic group or a thio-ether and a heterocyclic nitrogen or sulphur compound, or that the ligand instead of the thio-ether contains an amine or a sulphone group in combination with an aromatic or heterocyclic nitrogen or sulphur compound. The adsorption takes place in a salt environment, using antichaotropic salts such as sulphate, phosphate, chloride and the salts of polybasic acids, e.g. citrate, which promote adsorption at mol concentrations exceeding 1/5 of their solubility in water. Low-molecular substances in complex with proteins can be separated in accordance with the invention, but it is preferably used to separate protein-protein complexes in which both components have a molecular weight exceeding 10,000. However, the invention can also be applied to molecular weights as low as >100. The separation is preferably performed in a pH interval not exceeding 3 pH units from the isoelectric point of the complex. The barrier may be in the form of a thin layer, such as individual fibres, weave or paper, or it may be in the form of a solution in order to permit rapid adsorption, in which case it is precipitated out after the adsorption.
SE8800676A 1988-02-26 1988-02-26 MAKE SURE TO ADORABLE A COMPARATIVE PROTEIN COMPLEX, SPECIFIC SIGNIFICANT BY BIOSPECIFIC DETERMINATION METHODS SE462165B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE8800676A SE462165B (en) 1988-02-26 1988-02-26 MAKE SURE TO ADORABLE A COMPARATIVE PROTEIN COMPLEX, SPECIFIC SIGNIFICANT BY BIOSPECIFIC DETERMINATION METHODS
PCT/SE1989/000078 WO1989008257A1 (en) 1988-02-26 1989-02-24 Determining reactants and combinations of reactants involved in biospecific reactions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8800676A SE462165B (en) 1988-02-26 1988-02-26 MAKE SURE TO ADORABLE A COMPARATIVE PROTEIN COMPLEX, SPECIFIC SIGNIFICANT BY BIOSPECIFIC DETERMINATION METHODS

Publications (3)

Publication Number Publication Date
SE8800676D0 SE8800676D0 (en) 1988-02-26
SE8800676L true SE8800676L (en) 1989-08-27
SE462165B SE462165B (en) 1990-05-14

Family

ID=20371506

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8800676A SE462165B (en) 1988-02-26 1988-02-26 MAKE SURE TO ADORABLE A COMPARATIVE PROTEIN COMPLEX, SPECIFIC SIGNIFICANT BY BIOSPECIFIC DETERMINATION METHODS

Country Status (2)

Country Link
SE (1) SE462165B (en)
WO (1) WO1989008257A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0750086B2 (en) * 1989-09-13 1995-05-31 ダイソー株式会社 Chromatographic packing material and method for separating water-soluble organic compounds using the same
DK52791D0 (en) * 1991-03-22 1991-03-22 Kem En Tec As ADSORPTION MATRIX
SE9401960L (en) * 1994-06-07 1995-12-08 Sven Oscarsson Alkali resistant protein adsorbent
DE4436173C1 (en) * 1994-10-10 1996-09-05 Max Planck Gesellschaft Hydrophobically modified matrix surface for bio-sensor etc.
DE19623131C2 (en) * 1996-06-10 2001-10-31 Gerhard Harry Scholz Conjugate, process for its preparation and its use for binding substances
EP1386660B1 (en) * 1996-08-30 2009-09-02 Upfront Chromatography A/S Isolation of immunoglobulins
DE102005059217B4 (en) * 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Method and test kit for the separation, purification and recovery of long and short chain nucleic acids

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4432871A (en) * 1981-01-22 1984-02-21 Asahi Kasei Kogyo Kabushiki Kaisha Immune adsorbent, adsorbing device and blood purifying apparatus
US4430229A (en) * 1981-05-22 1984-02-07 Asahi Kasei Kogyo Kabushiki Kaisha Immune adsorbent and adsorbing device
JPS58170506A (en) * 1982-03-30 1983-10-07 Sumitomo Chem Co Ltd Treatment of mutagenic substance
SE470261B (en) * 1984-05-17 1993-12-20 Jerker Porath Adsorbent for the separation and immobilization of proteins, methods of preparing an adsorbent, and its use for biopolymer fractionation
SE470099B (en) * 1984-05-17 1993-11-08 Jerker Porath Sulfone activated thioether adsorbents for separation of eg protein
SE452557B (en) * 1984-10-30 1987-12-07 Exploaterings Ab Tbf AMPHIPATIC GEL PRODUCT FOR CHROMATOGRAPHIC AND BASIC ADSORPTION
GB2181840B (en) * 1985-10-16 1989-11-29 Farmos Group Ltd Method for the immunoassay of a macromolecular analyte

Also Published As

Publication number Publication date
SE8800676D0 (en) 1988-02-26
WO1989008257A1 (en) 1989-09-08
SE462165B (en) 1990-05-14

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