SE8501959D0 - PREPARATION OF A TISSUE PREPARATION - Google Patents
PREPARATION OF A TISSUE PREPARATIONInfo
- Publication number
- SE8501959D0 SE8501959D0 SE8501959A SE8501959A SE8501959D0 SE 8501959 D0 SE8501959 D0 SE 8501959D0 SE 8501959 A SE8501959 A SE 8501959A SE 8501959 A SE8501959 A SE 8501959A SE 8501959 D0 SE8501959 D0 SE 8501959D0
- Authority
- SE
- Sweden
- Prior art keywords
- tissue specimen
- bath
- pressure
- preparation
- tissue
- Prior art date
Links
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000012024 dehydrating agents Substances 0.000 abstract 2
- 238000007654 immersion Methods 0.000 abstract 1
- 229940057995 liquid paraffin Drugs 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000012188 paraffin wax Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
In a method of impregnating a tissue specimen with paraffin either directly after an optional treatment of the tissue specimen with a fixing agent or after the tissue specimen optionally has been treated with a fixing agent and/or a dehydrating agent an immersion of the tissue specimen into a bath of liquid paraffin takes place. The bath is kept below a predetermined temperature at which thermolabile components and properties of the tissue specimen undergo changes. The pressure of the bath is then lowered from atmospheric pressure to a predetermined pressure at which water present in the tissue specimen and/or, when appropriate, the dehydrating agent vaporizes, the pressure of the bath then being increased again to atmospheric pressure.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8501959A SE8501959L (en) | 1985-04-23 | 1985-04-23 | PREPARATION OF A TISSUE PREPARATION |
PCT/SE1986/000185 WO1986006479A1 (en) | 1985-04-23 | 1986-04-22 | Method of impregnating a tissue specimen with paraffin |
EP19860902936 EP0220269A1 (en) | 1985-04-23 | 1986-04-22 | Method of impregnating a tissue specimen with paraffin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8501959A SE8501959L (en) | 1985-04-23 | 1985-04-23 | PREPARATION OF A TISSUE PREPARATION |
Publications (2)
Publication Number | Publication Date |
---|---|
SE8501959D0 true SE8501959D0 (en) | 1985-04-23 |
SE8501959L SE8501959L (en) | 1986-10-24 |
Family
ID=20359937
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8501959A SE8501959L (en) | 1985-04-23 | 1985-04-23 | PREPARATION OF A TISSUE PREPARATION |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0220269A1 (en) |
SE (1) | SE8501959L (en) |
WO (1) | WO1986006479A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5089288A (en) * | 1989-06-24 | 1992-02-18 | Berger Hermann J | Method for impregnating tissue samples in paraffin |
EP0822403B1 (en) * | 1996-08-02 | 1998-07-29 | Milestone S.r.l. | Process for processing organic specimens |
US6793890B2 (en) | 1997-08-20 | 2004-09-21 | The University Of Miami | Rapid tissue processor |
ATE407353T1 (en) * | 1997-08-20 | 2008-09-15 | Univ Miami | HIGH-QUALITY CONTINUOUS PROCESS FOR FIXATION, DEHYDRATION, DEGREASING AND IMPREGNATION OF TISSUES |
EP1440320B1 (en) | 2001-10-01 | 2012-03-28 | Leica Biosystems Melbourne Pty Ltd | Processor for histological tissue specimen treatment |
US8554372B2 (en) | 2003-09-29 | 2013-10-08 | Leica Biosystems Melbourne Pty Ltd | System and method for histological tissue specimen processing |
US20090298172A1 (en) | 2008-05-28 | 2009-12-03 | Steven Paul Wheeler | Histological specimen treatment apparatus and method |
US10365189B2 (en) | 2015-05-07 | 2019-07-30 | Steven Wheeler | Histological specimen treatment |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2932112A1 (en) * | 1979-08-08 | 1981-02-26 | Carlos Prof Dr Thomas | METHOD AND DEVICE FOR EMBEDDING DRAINED TISSUE SAMPLES IN PARAFFIN |
-
1985
- 1985-04-23 SE SE8501959A patent/SE8501959L/en not_active Application Discontinuation
-
1986
- 1986-04-22 WO PCT/SE1986/000185 patent/WO1986006479A1/en unknown
- 1986-04-22 EP EP19860902936 patent/EP0220269A1/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
EP0220269A1 (en) | 1987-05-06 |
SE8501959L (en) | 1986-10-24 |
WO1986006479A1 (en) | 1986-11-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
NAV | Patent application has lapsed |
Ref document number: 8501959-4 Effective date: 19870110 |