SE8200403L - HYBRID CELLS, ANTIBODIES, KIT FOR PRODUCING HYBRID CELLS, PRODUCING AN ANTIBODY AND KIT FOR PRODUCING ANTIBODIES - Google Patents

HYBRID CELLS, ANTIBODIES, KIT FOR PRODUCING HYBRID CELLS, PRODUCING AN ANTIBODY AND KIT FOR PRODUCING ANTIBODIES

Info

Publication number
SE8200403L
SE8200403L SE8200403A SE8200403A SE8200403L SE 8200403 L SE8200403 L SE 8200403L SE 8200403 A SE8200403 A SE 8200403A SE 8200403 A SE8200403 A SE 8200403A SE 8200403 L SE8200403 L SE 8200403L
Authority
SE
Sweden
Prior art keywords
producing
hybrids
antibodies
hybrid cells
kit
Prior art date
Application number
SE8200403A
Other languages
Unknown language ( )
Swedish (sv)
Inventor
H-K Hochkeppel
Ley M De
Original Assignee
Biotechnolog Forschung Gmbh
Rega Instituut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19823200657 external-priority patent/DE3200657A1/en
Application filed by Biotechnolog Forschung Gmbh, Rega Instituut filed Critical Biotechnolog Forschung Gmbh
Publication of SE8200403L publication Critical patent/SE8200403L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/249—Interferons

Abstract

A process for the manufacture of hybrid cells capable of producing an antibody in which (a) mice are immunised with an antigen, (b) a lymphocyte mixture is isolated from the mice, (c) B lymphocytes are fused or hybridised with mouse B myeloma cells, (d) the mixture consisting of formed hybrids and non-hybridised B myeloma cells is conveyed into a culture medium for the formed hybrids and the formed hybrids are cultivated and selected with respect to the non- hybridised B myeloma cells, (e) they are subcloned to provide hybrids which are capable of producing the antibody which is directed specifically against the antigen, and (f) optionally the hybrids are isolated, characterised in that at step (d) the selection process is carried out in a medium containing methylcellulose (as hereinbefore defined), and hybrids selected at step (d) are optionally injected into the abdominal cavity of mice (which have previously been treated with a tumour stimulant), multiplied hybrids are removed from the abdominal cavity and subsequently the subcloning and isolating steps (d) and (f) are carried out. The invention also provides a process for producing monoclonal antibodies, and further provides hybrid cells capable of producing antibodies directed specifically against human interferon and provides such monoclonal antibodies.
SE8200403A 1981-01-28 1982-01-26 HYBRID CELLS, ANTIBODIES, KIT FOR PRODUCING HYBRID CELLS, PRODUCING AN ANTIBODY AND KIT FOR PRODUCING ANTIBODIES SE8200403L (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3102772 1981-01-28
DE3122832 1981-06-09
DE3143379 1981-11-02
DE19823200657 DE3200657A1 (en) 1981-01-28 1982-01-12 Process for the preparation of hybrid cells producing an antibody and of antibodies, hybrid cells and antibodies

Publications (1)

Publication Number Publication Date
SE8200403L true SE8200403L (en) 1982-07-29

Family

ID=27432623

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8200403A SE8200403L (en) 1981-01-28 1982-01-26 HYBRID CELLS, ANTIBODIES, KIT FOR PRODUCING HYBRID CELLS, PRODUCING AN ANTIBODY AND KIT FOR PRODUCING ANTIBODIES

Country Status (5)

Country Link
DK (1) DK37382A (en)
FI (1) FI820200L (en)
FR (1) FR2498626A1 (en)
GB (1) GB2092614A (en)
SE (1) SE8200403L (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3379055D1 (en) * 1982-06-09 1989-03-02 Asahi Chemical Ind Method for producing human antibody
JPS59172496A (en) * 1983-03-18 1984-09-29 Kyowa Hakko Kogyo Co Ltd Monoclonal antibody
US4599306A (en) * 1983-04-15 1986-07-08 Amgen Monoclonal antibodies which specifically bind to human immune interferon
CA1340698C (en) * 1986-07-25 1999-08-10 Kabushiki Kaisha Hayashibara Seibutsu Kaguku Kenkyujo Preparation and uses of interferon-gamma

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3884758A (en) * 1974-01-30 1975-05-20 Massachusetts Inst Technology Triglyceride-accumulating clonal cell line
GB1540579A (en) * 1975-08-01 1979-02-14 Searle & Co Cell culture medium
FR2492842A1 (en) * 1980-10-24 1982-04-30 Centre Nat Rech Scient Hybrid cells for human interferon antibodies - prepd. by fusing myeloma cells with mouse anti:interferon cells

Also Published As

Publication number Publication date
FI820200L (en) 1982-07-29
DK37382A (en) 1982-07-29
GB2092614A (en) 1982-08-18
FR2498626A1 (en) 1982-07-30

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