SE8100238L - SYNTHETIC GENES, PLASMID VECTORS INCLUDING THIS GENES, PROCEDURES FOR GENE AND VECTOR PREPARATION AND USE, AND SALUNDA RECEIVED CELLS AND PEPTIDES - Google Patents

SYNTHETIC GENES, PLASMID VECTORS INCLUDING THIS GENES, PROCEDURES FOR GENE AND VECTOR PREPARATION AND USE, AND SALUNDA RECEIVED CELLS AND PEPTIDES

Info

Publication number
SE8100238L
SE8100238L SE8100238A SE8100238A SE8100238L SE 8100238 L SE8100238 L SE 8100238L SE 8100238 A SE8100238 A SE 8100238A SE 8100238 A SE8100238 A SE 8100238A SE 8100238 L SE8100238 L SE 8100238L
Authority
SE
Sweden
Prior art keywords
genes
gene
plasmid vectors
peptides
salunda
Prior art date
Application number
SE8100238A
Other languages
Swedish (sv)
Other versions
SE451595B (en
Inventor
N H Carey
J S Emtage
M T Doel
M A William
Original Assignee
Searle & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Searle & Co filed Critical Searle & Co
Publication of SE8100238L publication Critical patent/SE8100238L/en
Publication of SE451595B publication Critical patent/SE451595B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C07K5/06113—Asp- or Asn-amino acid
    • C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
    • C07K5/0613—Aspartame
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

A synthetic gene which comprises, in the coding strand, codons for the amino acids of a desired peptide in tandem repeat is disclosed. A process for the production of such a synthetic gene which comprises the self-assembly and ligation and optionally repair of a plurality of appropriate oligonucleotides, the assembly to give the repeating character being achieved by self-complementary overlapping ends at the termini of the group of oligonucleotides which when assembled, comprises the repeating unit of the gene is also disclosed. Such synthetic genes may be used to produce recombinant plasmid vectors, to transform cell and hence to express peptides. Inter alia the present invention may be applied to the production of (aspartyl-phenylalanine)n and hence the sweetener "Aspartame".
SE8100238A 1980-01-30 1981-01-16 PLASMID VECTOR INCLUDING A SYNTHETIC GENE FOR EXPRESSION OF THE REPETITIVE POLYPEPTIDES (ASPARTYL-PHENYLALANIN) N, WHERE N IS AN INTEGRAL OF 2 AND E.COLI TRANSFORMED WITH THE VECTOR SE451595B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8003191 1980-01-30

Publications (2)

Publication Number Publication Date
SE8100238L true SE8100238L (en) 1981-07-31
SE451595B SE451595B (en) 1987-10-19

Family

ID=10511011

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8100238A SE451595B (en) 1980-01-30 1981-01-16 PLASMID VECTOR INCLUDING A SYNTHETIC GENE FOR EXPRESSION OF THE REPETITIVE POLYPEPTIDES (ASPARTYL-PHENYLALANIN) N, WHERE N IS AN INTEGRAL OF 2 AND E.COLI TRANSFORMED WITH THE VECTOR

Country Status (14)

Country Link
JP (1) JPS579795A (en)
AU (1) AU545908B2 (en)
BE (1) BE887290A (en)
CA (1) CA1189466A (en)
CH (1) CH662821A5 (en)
DE (1) DE3102721A1 (en)
DK (1) DK17881A (en)
ES (1) ES8203965A1 (en)
FR (1) FR2479259B1 (en)
IE (1) IE50833B1 (en)
IL (1) IL61952A (en)
IT (1) IT1170653B (en)
NL (1) NL8100437A (en)
SE (1) SE451595B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2052516B (en) * 1979-06-01 1983-06-29 Searle & Co Plasmid vectors production and use thereof
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
AU5762699A (en) * 1998-09-19 2000-04-10 Sang Jun Lee Dna cassette encoding a multimer of a biologically active peptide and a cleavable linker attached thereto and process for preparing the biologically active peptide

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0036258A3 (en) * 1980-03-14 1982-02-10 Cetus Corporation Process for producing aspartame

Also Published As

Publication number Publication date
BE887290A (en) 1981-05-14
CA1189466A (en) 1985-06-25
JPS579795A (en) 1982-01-19
SE451595B (en) 1987-10-19
IL61952A0 (en) 1981-02-27
IE50833B1 (en) 1986-07-23
NL8100437A (en) 1981-09-01
FR2479259A1 (en) 1981-10-02
IE810071L (en) 1981-07-30
FR2479259B1 (en) 1985-07-12
IT8147639A0 (en) 1981-01-27
CH662821A5 (en) 1987-10-30
IL61952A (en) 1984-09-30
DE3102721C2 (en) 1988-11-10
DK17881A (en) 1981-07-31
AU6650781A (en) 1981-08-06
AU545908B2 (en) 1985-08-08
ES498948A0 (en) 1982-04-16
DE3102721A1 (en) 1982-01-07
ES8203965A1 (en) 1982-04-16
IT1170653B (en) 1987-06-03

Similar Documents

Publication Publication Date Title
ES8802249A1 (en) Molecular cloning and characterization of a further gene sequence coding for human relaxin.
ES8801676A1 (en) Molecular cloning and characterization of a gene sequence coding for human relaxin.
EP0305488A4 (en) Mycobacterial recombinants and peptides
MX9706050A (en) Bioactive fusion proteins and pre-existing tumor therapy.
ES2009238A6 (en) Mycobacterial recombinants and peptides
PL317722A1 (en) Sequences of synthetic leader peptides
CA2168429A1 (en) Expression of fusion polypeptides transported out of cytoplasm without leader sequences
EP0260148A3 (en) Improved recombinant expression method, vector and transformed cells
NO940736L (en) DNA sequences encoding gelonin polypeptide
CA2147623A1 (en) Novel p-selectin ligand protein
WO1996017941A3 (en) Production of c-terminal amidated peptides from recombinant protein constructs
EP1380648A3 (en) Recombinant DNAase B derived from streptococcus pyogenes
SE8100238L (en) SYNTHETIC GENES, PLASMID VECTORS INCLUDING THIS GENES, PROCEDURES FOR GENE AND VECTOR PREPARATION AND USE, AND SALUNDA RECEIVED CELLS AND PEPTIDES
WO1995030001A3 (en) Novel p-selectin ligand protein
AU5776799A (en) Modified hcv peptide vaccines
EP0237157A3 (en) Vector and method for achieving high level expression in cells
ATE109827T1 (en) METHOD FOR THE PRODUCTION OF PEPTIDES, RECOMBINANT PLASMID TO BE USED THEREFORE AND MYELOMA CELLS TRANSFORMED WITH SUCH PLASMID.
ES8503373A1 (en) Molecular cloning and characterization of the gene sequence coding for porcine relaxin.
PE34296A1 (en) PROCEDURE FOR EXTRA-CELLULAR EXPRESSION OF PROTEIN
ES2008391A6 (en) DNA Encoding isopenicillin N-synthetase from A.Nidulans, its use and vectors encoding it.
RU93005218A (en) POLYEPEPTIDE, NUCLEOTIDE SEQUENCE, VECTOR, CELL, COMPOSITION, APPLICATION OF POLYPEPTIDE, METHOD FOR PRODUCING POLYPEPTIDE
WO2000053789A3 (en) Retroviral expression vectors on the basis of herv- long terminal repeat sequences
NZ536319A (en) Secreted and transmembrane polypeptides and nucleic acids encoding the same
NZ528704A (en) Novel PRO717 polypeptides and nucleic acids encoding a 12 transmembrane polypeptide
RU93032287A (en) METHOD OF OBTAINING OXYTOCINE AND PTOMEILOX RECOMBINANT PLASMID DNA AND ESCHERICHIA COLI TG1 / PTOTEILOX STRAINS FOR ITS IMPLEMENTATION

Legal Events

Date Code Title Description
NUG Patent has lapsed

Ref document number: 8100238-8

Effective date: 19910805

Format of ref document f/p: F