SE462004B - Foerfarande och komposition foer bestaemning av mikrobiella foeroreningar i spannmaal med hjaelp av polymera tvaafassystem - Google Patents
Foerfarande och komposition foer bestaemning av mikrobiella foeroreningar i spannmaal med hjaelp av polymera tvaafassystemInfo
- Publication number
- SE462004B SE462004B SE8803195A SE8803195A SE462004B SE 462004 B SE462004 B SE 462004B SE 8803195 A SE8803195 A SE 8803195A SE 8803195 A SE8803195 A SE 8803195A SE 462004 B SE462004 B SE 462004B
- Authority
- SE
- Sweden
- Prior art keywords
- phase
- polymeric
- sample
- microbial
- contaminants
- Prior art date
Links
- 235000013339 cereals Nutrition 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000000203 mixture Substances 0.000 title claims abstract description 9
- 230000000813 microbial effect Effects 0.000 title claims description 10
- 239000003344 environmental pollutant Substances 0.000 title 1
- 230000002538 fungal effect Effects 0.000 claims abstract description 52
- 230000001580 bacterial effect Effects 0.000 claims abstract description 31
- 238000000605 extraction Methods 0.000 claims abstract description 21
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000012569 microbial contaminant Substances 0.000 claims abstract 13
- 239000000356 contaminant Substances 0.000 claims abstract 5
- 235000011868 grain product Nutrition 0.000 claims abstract 4
- 239000007788 liquid Substances 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 16
- 230000003287 optical effect Effects 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 229920002307 Dextran Polymers 0.000 claims description 7
- 229920002472 Starch Polymers 0.000 claims description 5
- 229920000642 polymer Polymers 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 239000000523 sample Substances 0.000 claims 10
- 229910052783 alkali metal Inorganic materials 0.000 claims 3
- -1 alkali metal salt Chemical class 0.000 claims 3
- 150000004676 glycans Chemical class 0.000 claims 3
- 229920001515 polyalkylene glycol Polymers 0.000 claims 3
- 229920001282 polysaccharide Polymers 0.000 claims 3
- 239000005017 polysaccharide Substances 0.000 claims 3
- 239000012488 sample solution Substances 0.000 claims 3
- 230000002378 acidificating effect Effects 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 2
- 230000002708 enhancing effect Effects 0.000 claims 2
- 239000012535 impurity Substances 0.000 claims 2
- 229910017053 inorganic salt Inorganic materials 0.000 claims 2
- 229920001542 oligosaccharide Polymers 0.000 claims 2
- 150000002482 oligosaccharides Chemical class 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 239000004094 surface-active agent Substances 0.000 claims 1
- 238000002835 absorbance Methods 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 238000005259 measurement Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 3
- 239000004353 Polyethylene glycol 8000 Substances 0.000 description 3
- 239000008364 bulk solution Substances 0.000 description 3
- 239000007979 citrate buffer Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000001317 epifluorescence microscopy Methods 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 229940085678 polyethylene glycol 8000 Drugs 0.000 description 3
- 235000019446 polyethylene glycol 8000 Nutrition 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000011005 laboratory method Methods 0.000 description 2
- INHCSSUBVCNVSK-UHFFFAOYSA-L lithium sulfate Chemical compound [Li+].[Li+].[O-]S([O-])(=O)=O INHCSSUBVCNVSK-UHFFFAOYSA-L 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 229920002491 Diethylaminoethyl-dextran Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000228145 Penicillium brevicompactum Species 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011491 glass wool Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229940038580 oat bran Drugs 0.000 description 1
- 229940098377 penicillium brevicompactum Drugs 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 238000000711 polarimetry Methods 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000007430 reference method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/10—Starch-containing substances, e.g. dough
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N25/00—Investigating or analyzing materials by the use of thermal means
- G01N25/14—Investigating or analyzing materials by the use of thermal means by using distillation, extraction, sublimation, condensation, freezing, or crystallisation
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8803195A SE462004B (sv) | 1988-09-12 | 1988-09-12 | Foerfarande och komposition foer bestaemning av mikrobiella foeroreningar i spannmaal med hjaelp av polymera tvaafassystem |
| PCT/SE1989/000487 WO1990002948A1 (en) | 1988-09-12 | 1989-09-12 | A method and a compositon for the determination of microbial contamination in grain using polymeric two-phase systems |
| AU42208/89A AU4220889A (en) | 1988-09-12 | 1989-09-12 | A method and a composition for the determination of microbialcontamination in grain using polymeric two-phase systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8803195A SE462004B (sv) | 1988-09-12 | 1988-09-12 | Foerfarande och komposition foer bestaemning av mikrobiella foeroreningar i spannmaal med hjaelp av polymera tvaafassystem |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE8803195D0 SE8803195D0 (sv) | 1988-09-12 |
| SE8803195L SE8803195L (OSRAM) | 1990-03-13 |
| SE462004B true SE462004B (sv) | 1990-04-23 |
Family
ID=20373292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8803195A SE462004B (sv) | 1988-09-12 | 1988-09-12 | Foerfarande och komposition foer bestaemning av mikrobiella foeroreningar i spannmaal med hjaelp av polymera tvaafassystem |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU4220889A (OSRAM) |
| SE (1) | SE462004B (OSRAM) |
| WO (1) | WO1990002948A1 (OSRAM) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2186355A1 (en) * | 1994-05-23 | 1995-11-30 | Alexander Demetrios Kopatsis | Method for the rapid separation and identification of microbial contaminants from a complex matrix |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3981777A (en) * | 1976-01-19 | 1976-09-21 | Union Carbide Corporation | Method and apparatus for testing materials for levels of agents inhibitory or toxic to microorganisms |
| WO1982001563A1 (en) * | 1980-10-23 | 1982-05-13 | Bo Mattiasson | Biological and chemical conversion processes in liquid phase-system |
| FR2587722B1 (fr) * | 1985-09-25 | 1988-05-20 | Rhone Poulenc Agrochimie | Procede de diagnostic des maladies fongiques des plantes et dispositif pour la mise en oeuvre de ce procede |
-
1988
- 1988-09-12 SE SE8803195A patent/SE462004B/sv not_active IP Right Cessation
-
1989
- 1989-09-12 WO PCT/SE1989/000487 patent/WO1990002948A1/en not_active Ceased
- 1989-09-12 AU AU42208/89A patent/AU4220889A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| SE8803195D0 (sv) | 1988-09-12 |
| SE8803195L (OSRAM) | 1990-03-13 |
| AU4220889A (en) | 1990-04-02 |
| WO1990002948A1 (en) | 1990-03-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAL | Patent in force |
Ref document number: 8803195-0 Format of ref document f/p: F |
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| NUG | Patent has lapsed |