SE304488B - - Google Patents

Info

Publication number
SE304488B
SE304488B SE6073/64A SE607364A SE304488B SE 304488 B SE304488 B SE 304488B SE 6073/64 A SE6073/64 A SE 6073/64A SE 607364 A SE607364 A SE 607364A SE 304488 B SE304488 B SE 304488B
Authority
SE
Sweden
Prior art keywords
bacteria
hydrocarbon
feeds
inorganic salts
aqueous
Prior art date
Application number
SE6073/64A
Inventor
K Guenther
M Perkins
Original Assignee
Exxon Research Engineering Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US360141A external-priority patent/US3384491A/en
Application filed by Exxon Research Engineering Co filed Critical Exxon Research Engineering Co
Publication of SE304488B publication Critical patent/SE304488B/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26Processes using, or culture media containing, hydrocarbons
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L35/00Food or foodstuffs not provided for in groups A23L5/00 – A23L33/00; Preparation or treatment thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A process for preparing a high protein food comprises continuously supplying a C1-C30 hydrocarbon a non-limiting aqueous inorganic salts growth medium and excess oxygen-containing gas to a vigorously stirred reactor containing the hydrocarbon, aqueous medium and non-toxic hydrocarbon-consuming bacteria, the bacteria being in the phase (population increasing exponentially) of growth and being present in a concentration equal to or greater than that for which the exponential increase in population increment is equal to the increment continuously withdrawn, continuously removing a mixture of bacteria, aqueous medium and unconverted hydrocarbon, and separating the bacteria to obtain directly the proteinaceous food. The material separated from bacteria may be recycled to the reactor. Under preferred conditions the liquid residence time is 1.5 to 4 times the minimum generation time for the particular bacteria, and the oxygen-containing gas supplied to the reactor is 0.5-4 volumes of air/volume reactor liquid/minute. Thus Micrococcus cerificans may be cultivated with a residence time of 1.0-10 hours on a mixture of 0.1 to 10 weight % of a 190 DEG -400 DEG C. boiling normal paraffin feed, containing less than 0.1 weight % aromatics in an inorganic salts aqueous growth medium oxygen-enriched by air containing more than 90% of oxygen amounting to 1.5-2.5 lbs. per lb. dried bacteria recovered, and cells of the bacteria containing 72-93 weight % liquid based on dry cells are separated from the continuously removed mixture of bacteria, aqueous medium and unconverted paraffin with the aid of a cationic surfactant and dried. The composition of the inorganic salts aqueous growth medium is disclosed in detail. The final product cells after drying contain 7-10 weight % of total fat of which about 75% is made up of esters, and 5-9 weight % of inorganic salts. The C1-C30 hydrocarbon feeds are preferably gas oils boiling in the range of 190 DEG -400 DEG C. Other feeds are C1-C30 normal paraffins, isoparaffins, cycloparaffins, monoolefins, diolefins and aromatics. Feeds particularly suitable are C11-C30 normal paraffins from gas oils, light naphthas, and normally gaseous feeds such as methane, ethane, propane and natural gas. In the cultivation of Micrococcus cerificans any C6-C30 feed will require purification by a molecular sieve process to reduce the level of both mono- and poly-cyclic aromatics to below 0.5 weight %. The following species of bacteria are preferably employed: Micrococcus cerificans, Pseudomonas aeruginosa, Pseudomonas fluorescens, Nocardia opaca, Nocardia rubra, Nocardia coralina, Pseudomonas methanica, Pseudomonas desmolyticum and Mycobacterium phleie. Certain examples are directed to the effect of different feeds viz. gas oil fraction, slack wax and hexadecane, on bacterial yield and of the proteinaceous product upon animal growth (rats) in comparison with casein. It is stated that the unreacted hydrocarbon residues from the gas oil and slack wax are animal growth inhibitors.ALSO:A process for preparing a high protein food comprises continuously supplying a C1-C30 hydrocarbon, a non-limiting aqueous inorganic salts growth medium and excess oxygen-containing gas to a vigorously stirred reactor containing the hydrocarbon, aqueous medium and non-toxic hydrocarbon-consuming bacteria, the bacteria being in the log phase (population increasing exponentially) of growth and being present in a concentration equal to or greater than that for which the exponential increase in population increment is equal to the increment continuously withdrawn, continuously removing a mixture of bacteria, aqueous medium and unconverted hydrocarbon, and separating the bacteria to obtain directly the proteinaceous food. The material separated from bacteria may be recycled to the reactor. Under preferred conditions the liquid residence time is 1.5 to 4 times the minimum generation time for the particular bacteria, and the oxygen-containing gas supplied to the reactor is 0.5-4 volumes of air/volume reactor liquid minute. Thus Micrococcus cerificans may be cultivated with a residence time of 0.1-10 hours on a mixture of 0.1 to 10 weight per cent. of a 190 DEG -400 DEG C. boiling normal paraffin feed, containing less than 0.1 weight per cent. aromatics, in an inorganic salts aqueous growth medium oxygen-enriched by air containing more than 90% of oxygen amounting to 1.5-2.5 lbs. per lb. dried bacteria recovered, and cells of the bacteria containing 72-93 weight per cent. liquid based on dry cells are separated from the continuously removed mixture of bacteria, aqueous medium and unconverted paraffin with the aid of a cationic surfactant and dried. The composition of the inorganic salts aqueous growth medium is disclosed in detail. The final product cells after drying contain 7-10 weight per cent. of total fat of which about 75% is made up of esters, and 5-9 weight per cent. of inorganic salts. The C1-C30 hydrocarbon feeds are preferably gas oils boiling in the range of 190 DEG -400 DEG C. Other feeds are the C1-C30 normal paraffins, isoparaffins, cycloparaffins, mono-olefins, diolefins and aromatics. Feeds particularly suitable are C11-C30 normal paraffins from gas oils, light naphthas, and normally gaseous feeds such as methane, ethane, propane and natural gas. In the cultivation of Micrococcus cerificans any C6-C30 feed will require purification by a molecular sieve process to reduce the level of both mono- and polycyclic aromatics to below 0.5 weight per cent. The following species of bacteria are preferably employed: Micrococcus cerificans, Pseudomonas aeruginosa, Pseudomonas fluorescens, Nocardia opaca, Nocardia rubra, Nocardia coralina, Pseudomonas methanica, Pseudomonas desmolyticum and Mycobacterium phleie. Certain examples are directed to the effect of different feeds viz. gas oil fraction, slack wax and hexadecane, on bacterial yield and of the proteinaceous product upon animal growth (rats) in comparison with casein. It is stated that the unreacted hydrocarbon residues from the gas oil and slack wax are animal growth inhibitiors.
SE6073/64A 1963-05-21 1964-05-19 SE304488B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28189563A 1963-05-21 1963-05-21
US360141A US3384491A (en) 1964-04-15 1964-04-15 Process for producing high protein feed supplements from hydrocarbons

Publications (1)

Publication Number Publication Date
SE304488B true SE304488B (en) 1968-09-30

Family

ID=26961138

Family Applications (1)

Application Number Title Priority Date Filing Date
SE6073/64A SE304488B (en) 1963-05-21 1964-05-19

Country Status (5)

Country Link
JP (1) JPS5324520B1 (en)
DE (1) DE1442089A1 (en)
ES (1) ES300101A1 (en)
GB (1) GB1062816A (en)
SE (1) SE304488B (en)

Also Published As

Publication number Publication date
GB1062816A (en) 1967-03-22
JPS5324520B1 (en) 1978-07-21
ES300101A1 (en) 1964-11-16
DE1442089A1 (en) 1969-03-06

Similar Documents

Publication Publication Date Title
US4317843A (en) Microbiological production of protein
SU706026A3 (en) Method of culturing microorganisms
Whiting et al. Metabolism of cinnamic acid and hydroxy-cinnamic acids by Lactobacillus pastorianus var. quinicus
US3384491A (en) Process for producing high protein feed supplements from hydrocarbons
SE304488B (en)
US3989594A (en) Microbiological production of protein
US3474001A (en) Growing microorganisms on hydrocarbons
US3616216A (en) Production recovery and application of enzymatically active micro-organisms
US3622465A (en) Protein from normal hydrocarbons
EP0098137A3 (en) A microbiological process for the oxidation of alkanes, vinyl compounds and secondary alcohols
US3620927A (en) Cultivation of micro-organisms on hydrocarbons
Takahashi et al. Studies on the Utilization of Hydrocarbons by Microorganisms: Part V Screening of Yeasts for Cell Production from Hydrocarbons and their RNA Contents
US3674640A (en) Cultivation of hydrocarbon-consuming yeasts
Tada et al. Mechanism of photoregulated carotenogenesis in Rhodotorula minuta II. Aspects of photoregulative reaction
US3525671A (en) Anaerobic photosynthetic fermentation of hydrocarbons
US3676299A (en) Microbial production of novel hydroxyphenyl ketobutyric acids
Tilton et al. MARINE THIOBACILLI: II. CULTURE AND ULTRASTRUCTURE
GB1197236A (en) Fermentation of Hydrocarbons
US3991112A (en) Methylated muconic acid hydrazides
US3775089A (en) Methylated muconic acid hydrazides
US3301766A (en) Fermentation process for preparing cinnamic acid and 5-phenyl valeric acid
Kusk Effects of naphthalene on the diatom Phaeodactylum tricornutum grown under varied conditions
USRE30965E (en) Fermentation of oxygenated hydrocarbon compounds with thermophilic microorganisms and microorganisms therefor
Vanzella et al. Recovery of nitrification in marine bacteria following exposure to carbon monoxide or light.
US3510401A (en) Production,recovery and application of enzymatically active microoganisms