SE199740C1

SE199740C1 -

Info

Publication number: SE199740C1
Authority: SE
Publication date: 1965-01-01

Description

KLASS INTERNATIONELLSVENSK A61 k30h:14 PATENT- OCH REGISTRERINGSVERKET Ans. 291/1959 inkom den 13/1 1959 utlagd den 15/2 196 ANKH LABORATORIES, INC., FAIRBORN, OHIO USA Apparat for undersokning av metaboliska amnens inverkan ph bakterieorganismer Uppfinuare: A B Salisbury jr Deana uppfinning hanfor ,sig allmant till apparater avs,edda att anvandas vid bakteriologiska kulturstudier och i synnerhet till apparater, i vilka verkningarna av olika metaboliska amnen pa bakterier bestammas genomi iakttagande av tillvaxten av hakterier I ett ndringskulturmedium, sedan amnena bringats i kontakt med en yta av mediet, som i forvag infekterats med bakterierna. Det allmanna andamdlet med uppfinningen ãr att astadkom, ma en fly kulturstudiumapparat, vilken forenklar och minskar antalet op,erationer, som erforciras for ett fullstandigt bakteriologiskt kulturstudiuin av ovan angivet slag, vilken är enkel och billig att tillverka och vilken ken anvandas for .fullstandiga kulturstudier Irian ytterligare oiler speciell lahoratorieapparatur. Ett annat andamal dr .att i 'fiirvag forpacka ett faertal olika metaboliska amen och kulturmediet i en. enhet, vilken ekonomiskt kan hortkastas efter en enda :anvandnin,g. Ett annat andamal är att upp:bara de metaboliska amnena i locket till en behallare fOr kulturmediet pa ett nytt salt far att hringa medlen i riktig kontakt med rnediet till faljd •av hopsattrting av locket och behallaren. Uppfinningen. bestar Oven i en ny konstruktion air behallare,delarna och ett nytt arrangemang av de metaboliska amnena i locket far att underlatta visuellt iakttagande av amnenas verknin gar. CLASS INTERNATIONAL SWEDISH A61 k30h: 14 PATENT AND REGISTRATION AGENCY Ans. 291/1959 was received on 13/1 1959 published on 15/2 196 ANKH LABORATORIES, INC., FAIRBORN, OHIO USA Apparatus for investigating the effect of metabolic substances on bacterial organisms Inventor: AB Salisbury jr to be used in bacteriological culture studies and in particular to apparatus in which the effects of various metabolic substances on bacteria are determined by observing the growth of bacteria in a culture medium of change, after the substances have been brought into contact with a surface of the medium previously infected with the bacteria. The general object of the invention is to provide a flexible culture study apparatus which simplifies and reduces the number of operations required for a complete bacteriological culture study of the above kind, which is simple and inexpensive to manufacture and which can be used for complete culture studies. Irian further oiler special laboratory equipment. Another goal is to package a number of different metabolic amen and culture media in one. unit, which can be economically discarded after a single: use, g. Another purpose is to: only the metabolic substances in the lid of a container for the culture medium on a new salt may circulate the means in proper contact with the container as a result of • assembling the lid and the container. The invention. In addition to a new construction, air containers, the parts and a new arrangement of the metabolic substances in the lid make it easier to visually observe the effects of the substances.

Uppfinningen beskrives, i det foljande ndrmare i anslutning till de hifogade ritningarna. Pa dessa dr fig. 1 en p,erspektivbild air en kulturstudiumapparat enligt denna uppfinning, varvid delarna visas sdsom de arc forpackade fore anvandningen. Fig. 2 är en delsektion i starre .skala efter linjen 2-2 pd. fig. 1,. Fig. 3 är en Mid liknande fig. 2 med delarna isdrtagna och visar hehdllarens delar fardiga for hopsattning. Fig. 4 ar en delbilcl after linjen 4-4 pa fig. 2. Fig. 5 Or en bild liknande fig. 3 med behallarens delar hopsatta. Fig. 6 är en hild underifran av behallaren med locket airlagsuat. Fig. 7 ãr en delbild underifran. air be,. hall:arm riled locket pasiatt cob. hottnen partient avlagsnad. Fig. 8 och aro delbilder liknande fig. , air modifierade utforingsformer av uppfinningen. Fig. 10 Or en delsektion som vis:ar en -annan. modifikation. The invention is described in more detail below in connection with the accompanying drawings. In these, Fig. 1 is a perspective view of a culture study apparatus according to this invention, the parts being shown as being packaged for use. Fig. 2 is a subsection on a rigid scale along the line 2-2 pd. Fig. 1 ,. Fig. 3 is a Mid similar to Fig. 2 with the parts disassembled and showing the parts of the holder ready for assembly. Fig. 4 is a fragmentary view taken along line 4-4 of Fig. 2. Fig. 5 is a view similar to Fig. 3 with the parts of the container assembled. Fig. 6 is a hollow bottom of the container with the airlagsuat lid. Fig. 7 is a partial view from below. air be ,. hall: arm riled lid pasiatt cob. hottnen partient aflagsnad. Fig. 8 and are partial views similar to Figs., Modified embodiments of the invention. Fig. 10 Or a sub-section showing a -another. modification.

Uppfinningen visas pd. ritningarna -for hely:- sande andamal i form av en kulturstudium, ap,parat omfattande en lag upptill oppen skal 11 lamplig for upptagande av en portion 12 av naringskulturinedium, sasom blod-agar, och Bled .ett last passande lock 13. Derma apparat Or speciellt lamplig for anyandning vid bakteriologiska kulturstudier for bestanming av verkningarna av olika metaboliska anmen pa bakterietillvaxten. Vid dessa studier infektern en blottad yta 14 av inediet 12 med halt, terieorganismer och portioner 15 av de olika :Lunen, som skola provas, hringas I kontakt med den infekterade ytan pd atskilda stallen., varvid avstandet mellan kontaktpunkterna for de olika imnena Or tillrOokllgt fOr att undvika overlappning .a5V dinn.enas effektivitetsZO, ner. Ett avstdnd av 3 cm mellan mitten air narbeldgna zongrupper har visat sig tillrdckligt for detta andamdl. Eller en o,dlingstid, vilken har en langd, ,som bestammes, i Overens-stammelse med kand praxis och under vilken skIlen 11 Or thckt, iakttages, kulturen vi.-audit. Nar det Or fraga om, metaholiska Om-flea, vilka arc katabologiska, exempelvis antibiotika s:dsom kloramfenikol och oxitetracydin -eller kemoterap-eutika sasom 'sulfonamider, framtrader effektiviteten av varje amne gen.orn franvaro av bakterietillvaxt omkring den del air .mediets, yta, som Or i kontakt pied anmet, varvid det Oiler att ju effektivare am-net Or, ,desto stOrre blir det 0,mrade o-mkring dinnet dO.r tillvdxten Or inhiberad. Nar anab,oliska amnen, &born, vitaminer, proteiner 2— — och kolhydrater, anvandas., ger sig amnets effektivitet till karma som en Okning .av tfflväxten, varvid de effektivare amnena ha storre tillvaxtomraden omkring sina kontaktpunkter med mediet. The invention is shown pd. the drawings -for hely: - true andamal in the form of a cultural study, ap, prepared comprising a layer at the top of open shell 11 suitable for accommodating a portion 12 of nourishing culture medium, such as blood-agar, and Bled .a load fitting lid 13. Derma apparatus Is particularly suitable for use in bacteriological culture studies to determine the effects of various metabolic pathways on bacterial growth. In these studies, the infect a exposed surface 14 of the inediet 12 with contents, terry organisms and portions 15 of the various: to avoid overlap .a5V dinn.enas efficiencyZO, down. A distance of 3 cm between the mid-air zone zones has been shown to be sufficient for this purpose. Or a period of development, which has a length, which is determined, in accordance with kand practice and during which the difference 11 Or thckt, is observed, the culture vi.-audit. When it comes to metaholic Om-flea, which are catabolic, for example antibiotics such as chloramphenicol and oxytetracycline or chemotherapeutic agents such as sulfonamides, the effectiveness of each substance in the absence of bacterial growth around the part of the air medium is apparent. surface, which Or in contact pied anmet, whereby it Oiler that the more effective am-net Or,, the larger it becomes 0, mrade o-mkring dinnet dO.r tillvdxten Or inhibited. When anab, the oily substances, & children, vitamins, proteins 2— - and carbohydrates, are used.

Enligt denna uppfinning aro. locket 13 och skalen. 11 konstruerade pa ett nytt salt for att forenkla kulturstudier av ovan angivet slag och majliggara deras utforande aned hjalp av otranad personal.. For detta andamal uppbaras portionerna 15 av de olika raetaholiska amnen, som skala anvandas vid undersOkningen, av locket for kontakt II-led den exponerade ytaa 14 alr mediet 12 i skalen saint Oro atskilda i locket i overensstammelse med ett bestamt manster. Endera locket eller skalen innefattar aven medel for avgransning av ett flertal grupper 16 (fig. 6 och 7) av koncentriska effektivitetszoner till antalet motsvarande anmena ;och aTrangerade i ett motsvarande monster sa..att de hefinna sig mitt for mitten av de olika zongrupperna med de respektive anmena i locket, nth- det senare .anbringas pa skalen, varvid organet med zonerna är tillrackligt genomskinligt for visuellt iakttagande av bakterietillvaxten i var och en av zongrupperna. For att majliggora shdant iakttagande, aven nar locket Or avlagsnat, är det lampligast att utmarka zonerna pa. skalen saSOM visats; p0. ritningen. Skalen 11 omfattar i detta fall en hottenskiva 17 och en uppatriktad flans: 18. runt skhlens periferi far upptagande av kulturmediet 12, -vilket utgor ett tunt skikt som, tacker bottenskivan. FOr .att mojliggara undersokning av ett start antal Lunen 15, °eh darigenont utnyttja det exponerade mediets yta 14 effektivt, Or det lampligt att utfora skalen i avlang rektangular form med harnen arvrundade sa att de ansluta sig till formen hos de narbelagna effektivitetszonerna, sasoin visas pa ritningarna (fig. 6). Har Or skivan framstalld genomn pressning av ett larapligt termoplastiskt harts, sh.som klar polystyren. Gruppema 16 air effektivitetszoner Ore cirkelformiga och utmarkas i detta fall av linjer 19, som ritsats pa, undersidan av battenskivan medels,t ett Titsverktyg efter pressningen. Far att passa till skalen 11 har locket 13 likadan. avlang rektangular form med lavrundade horn. Locket kan vara anordnat att passe innanfor skalflansen. 18, men i delta fall cm-fatter locket en plan skiva: 20, .som Or obetydligt storre On bottenskivan 17, sa att den viler pa den byre kanten av skalens flans, 18, och ar forsedd med en nedskjutande flans. 21, som lost kan skjutas ned utanfor skalens. flans (fig. 5),. Portionerna 15 av de olika metaboliska amnena uppbaras; ph undersidan av lockets, skiva 20 med sina nedat vanda ytor p0. avstand fran skivan, varigenom kontakt med den blottade mediumytan sakerstalles. Har nppbares varje amne vid den nedre anden av ett &tad 22, s.ont straeker sig, nedat frart lockets skiva ett avsevart ,styeke far att skiktet av kulturmedium skall kunna vara tunt och darigenom mangden av och kostnaderna for media minskas. According to this invention aro. lid 13 and shells. 11 constructed on a new salt to simplify cultural studies of the kind indicated above and may be carried out with the help of untrained personnel. the exposed surface 14 alr the medium 12 in the scales saint oro separated in the lid in accordance with a particular pattern. Either the lid or the shells also includes means for defining a plurality of groups 16 (Figs. 6 and 7) of concentric efficiency zones to the number of corresponding objects; the respective members of the lid, the latter being applied to the shells, the organ with the zones being sufficiently transparent for visual observation of the bacterial growth in each of the zone groups. In order to make this observable, even when the lid has been removed, it is most appropriate to mark the zones on. the scales as shown; p0. the drawing. The shell 11 in this case comprises a hot plate 17 and an upwardly directed flange: 18. around the periphery of the shell is received the culture medium 12, which forms a thin layer which, thanks to the bottom plate. For the possible examination of a starting number of Lunen 15, ° eh darigenont utilize the surface 14 of the exposed medium effectively. on the drawings (Fig. 6). Has the Or board produced by pressing a flexible thermoplastic resin, such as clear polystyrene. The groups 16 air efficiency zones are circular and are in this case marked by lines 19, which are drawn on, the underside of the batt disc by means of a Tits tool after pressing. If it fits the shells 11, the lid 13 has the same. oblong rectangular shape with low-rounded horns. The lid can be arranged to fit inside the shell flange. 18, but in some cases the lid has a flat disc: 20, which is slightly larger on the bottom disc 17, so that it rests on the straight edge of the flange of the shell, 18, and is provided with a projecting flange. 21, as lost can be shot down outside the shell. flange (Fig. 5),. The portions of the various metabolic substances are carried; ph underside of the lid, disc 20 with its downturned surfaces p0. distance from the disc, thereby ensuring contact with the exposed medium surface. If each item is applied to the lower spirit of a & tad 22, as it were, down from the lid of the lid a considerable, styeke father that the layer of culture medium should be able to be thin and thereby reduce the amount and cost of media.

I den fOredragna app araten enligt fig. 1-7 Or konstruktionen_ av .stoden 22 forenklad go-nom att dessa pressats. i ett stycke med lockets skiva 20, vilken bestar av samma material som skalen 11. For att underlatta pressningen Oro .stoden utformade so,m ihaliga cylindriska ror eller tapp,ar. De senare Ore anordnade i ett monster, som motsvarar manstret for grupperna 16 -av effektivitetszoner, med fyra raw 22 anbragta p5. avstand fran varandra Fangs vardera sidokanten air lockets skiva 20 och tre utefter skivans langdaxel pa s,amma .a.vstand som gruppernas eentra. Portionerna 15 av de olika metaboliska amnena kunna anbringas. phde nedskjutande roren ph olika salt, exem.pelvis genom impregnering av stycken 23 av absorberande papper med arnnena och fastande av dessa stycken vid raren med ett lampligt Mister, sasom visas ph. fig. 8, eller genom. fyllning av den. ihaliga nedre anddelen .av varje rar, .sasom visas pa fig. 9', med en portion av amnet i .pulverform, fasthallet i roret medelst lampliga bindemedel sisom glycerol. Det foredrages •emellertid att urtaga och upprugga andarna av roren, sasom, visas ph fig. 2-5, ;ocli att fylla dessa urtagningar med en hlandning .av ett hindemedel och amnet i pulverform. Dessa urtagningar kunna &tadk.onurias genom sandblastring av rorens Ondar. Den uppruggade Auden av ett rOr visas, pi fig. 4 fare 'anbringandet av amnet darpa. In the preferred apparatus according to Figs. 1-7, the construction of the post 22 is simplified by pressing them. in one piece with the cover disc 20, which consists of the same material as the shells 11. To facilitate pressing, the column formed so-called cylindrical tubes or pins. The latter Ore arranged in a sample, corresponding to the pattern for the groups 16 - of efficiency zones, with four raw 22 placed p5. distance from each other Each side edge of the air lock disc 20 and three along the long axis of the disc are caught at the same distance as the one of the groups. The portions of the various metabolic substances can be applied. phde lowering the tubes ph different salt, for example by impregnating pieces 23 of absorbent paper with the hearths and attaching these pieces to the tube with a suitable Mister, as shown ph. Fig. 8, or through. filling it. The lower part of each tube, as shown in Fig. 9 ', with a portion of the substance in powder form, is retained in the tube by means of suitable binders such as glycerol. However, it is preferred to remove and roughen the spirits of the tubes, as shown in Figs. These recesses can be & tadk.onurias by sandblasting the Rorens Ondar. The roughened Auden of a pipe is shown, in Fig. 4 the application of the blank darpa.

Vid en ;alternativ konstruktion, Sall visas pa fig. 10, innefatta staden. 24 for amnena p,artiellt utskurna flikar, s.orn hafts ned frail en separat styv skiva 25 av lampligt material, sawin papp, vilken_ Or anbragt mot undersidan av sldvan 20 och innanfor flansen 21. Flikarna 'skjuta ned tillrackligt langt for att de portioner 15 am metaboliska amnen, som uppbaras av flikarnas nedre andar, skola Tura i kontakt med kulturmediet 12, ph. sanuna satt som amnena p5. roren 22 vid den. foredragna_ utforingsformen, nar lockets skiva anligger mot s•khlens, flans 18. Far att minska majligheterna till fel vid kulturstudier av ovan an-. givet slag och far att forenkla dessa studier ytterligare Ore locket 13 och skalen. 11 kenstruerade pS. ett nytt salt, vilket endast medger lockets anbringande p0. skalen i ett enda foruthestamt lage i forhallan.de till den senare. For detta andamhl ,skjuta olika antal av utsprang 26 (fig. 3) ut frau motsatta andar av skalen 11 i nkheten av dess botten och motsvarande antal urtagningar 27 Oro utformade i de nedskjutande andarna av lockets flans 21, i vilka utsprangen kunna inforas sk att lockets skiva kan bringas att anligga mot skalensr flans 18, nar lockets andar Oro orienterade riktigt invid de anotsvarande andarna_ av skalen, shaoni visas ph fig. 5. Nar locket vandes sh att motsvarande andar hos locket. — ,---3 och skalen icke samnianforas, komma utsprangen att forhindra att locket .skjute.s ned Over skalen sh att amnena 15 komma i kontakt med kulturmediet 12. In an alternative construction, Sall is shown in Fig. 10, including the city. 24 for the blanks on, articulately cut flaps, which have been lowered from a separate rigid disc 25 of suitable material, sawn cardboard, which is placed against the underside of the sleeve 20 and inside the flange 21. The flaps' slide down long enough for the portions 15 am metabolic substances, carried by the lower spirits of the lobes, school Tura in contact with the culture medium 12, ph. sanuna sat as amnena p5. rudder 22 at it. preferred_ embodiment, when the lid of the lid abuts against s • khlens, flange 18. Father to reduce the possibilities of errors in cultural studies of the above an-. given kind and father to simplify these studies further Ore lid 13 and the shells. 11 constructed pS. a new salt, which only allows the application of the lid p0. the shells of a single pre-existing layer in forhallan.de to the latter. For this purpose, project different numbers of projections 26 (Fig. 3) from opposite spirits of the shells 11 in the vicinity of its bottom and corresponding number of recesses 27. the disc of the lid can be brought to abut against the flange 18 of the shell, when the spirits of the lid are oriented right next to the corresponding spirits_ of the shells, shaoni is shown ph fig. 5. When the lid is used sh that corresponding spirits of the lid. -, --- 3 and the shells are not joined together, the protrusions come to prevent the lid from being pushed down.

Den ovan beskrivna kulturstucliumapparaten L. speciellt lamplig for forpackning som en sluten enhet 28, vilken ekonomiskt kan bortkastas efter en enda anvandning och med silken ett fullstandigt kulturstudium kan genomforas utan ytterligare laboratorieapparatur. Denna enhet omfattar skal.en 11 med kulturmediet 12 i ett .skikt pa bottenskivan 12 och locket 13 med portionerna 15 av olika metaboliska arnnen snbringade pa de framskjutande andarna av roren 22 samt hermetiskt Made fran mediet Mom en hermetiskt sluten behallare 29 (fig. 2). Enligt en fOrbdtt- railav uppfinningen finnes ett organ. 30, som astadkommer hermetisk tatning anellan medial och amnena Mom behallaren, och detta organ kan vara utformat pa olika satt, °eh det visas som exempel i detta fall sasont omfattande ett twit ark av rnetallfolie (fig. 2), som tacker skalens oppning och medelst lampligt klister ãr fast vid sin cankrets till den ovre anden air skalens flans 18. Foretradesvis stracker sig det tatande arket 30 nal forbi skalens flans och anligger mot en utat vand ansats 31, vilken fir utforma.d. lockets flans 2.1 genom urtagning av dess insida, sasom visas pa fig. 2. Locket kan salunda uppbaras av det tatande arket, vilket hailer amnena hermetiskt skilda fran kulturmediet 12 inom behallaren 29. I detta fall Sr den senare en sluten pase med bojliga vaggar av termoplastiskt harts, sasom polyeten. Vid, hopsattningen air enheten 28 beredes forst kulturmediet 12 under sterila betingelser sasom ett skikt pa bottenskivan 17 1 skalen oak under uppratthAllande av likadana betingelser anbringas det tatande arket 30 pa den Ovre anden av ,skalens. flans 18, 'sasom visas pa fig. 2. Darefter anbringas portionerna 15 air amnena p5. raren 22 under .sterila betingelser och locket anbringas over skalen sa att ansatserna 31 anligga mot det tatande arkets 30 onnkretskanter. Skalen med det darpa anbragta locket infordes darefter, sasom visas pa fig. 2, i behallaren 29 och den senare tillslutes tatt. For beredning av enheten 28 for kulturstuclium avlagsnas skalen 11 frau behallaren 29, det tatande arket avrives fran skalens flans 18, och den ovre ytan 14 air kulturmediet 12 infekteras med bakterier, som skola anvandas i kulturen. Darefter avagsnas locket 13 fran behallaren 29 och anbringas. p5. skalen 11 genom, att flansen 21 skjutes. Over flansen 18 och locket forflyttas neat fran det pa fig. 3 visade laget. Om locket anbringas det ratta laget i forhallande till skalen komma utsprangen 26 att inforas i urtagningarna 27, varigenom lockets, skiva 20 kommer att anligga mot den owe ,kanten air skalens flans 18 med de metaholiska amnena 15 1 kontakt med ytart av kul- turmediet, .sasom visas pa fig. 5. Om locket anbringas felaktigt, komma utsprangert 26 nit stota mat den nedre delen air lockets flans och begransa lockets fOrskjutning nedat till ett lage, i vilket a.umena befinna sig pa av -stand fran kulturmediet. The culture stuclium apparatus L. described above is particularly suitable for packaging as a closed unit 28, which can be economically discarded after a single use and with the silk a complete culture study can be carried out without additional laboratory apparatus. This unit comprises the shell 11 with the culture medium 12 in a layer on the bottom plate 12 and the lid 13 with the portions 15 of different metabolic wounds wound on the projecting spirits of the tubes 22 and hermetically made from the medium Mom a hermetically sealed container 29 (Fig. 2). ). According to a forbdtt- rail of the invention there is a member. 30, which provides a hermetic seal between the medial and the parts of the container, and this means may be designed in different ways, although it is shown by way of example in this case comprising a twit sheet of metal foil (Fig. 2), which thanks to the opening of the shells and by means of a lamp adhesive is attached to its can circuit to the flange 18 of the upper second air shell. the flange 2.1 of the lid by removing its inside, as shown in Fig. 2. The lid can thus be supported by the sealing sheet, which holds the blanks hermetically separated from the culture medium 12 inside the container 29. In this case the latter is a closed passage with flexible cradles of thermoplastic resin, such as polyethylene. In the assembly of the unit 28, the culture medium 12 is first prepared under sterile conditions such as a layer on the bottom plate 17 in the shells and under the same conditions, the sealing sheet 30 is applied to the upper end of the shell. flange 18, as shown in Fig. 2. Thereafter, the portions 15 are applied to the air blanks p5. The lid 22 is placed under sterile conditions and the lid is applied over the shells so that the shoulders 31 abut against the vertical edges of the sealing sheet 30. The shell with the lid fitted is then inserted, as shown in Fig. 2, into the container 29 and the latter is closed. To prepare the culture stuclium unit 28, the shells 11 are removed from the container 29, the absorbent sheet is torn from the flange 18 of the shells, and the upper surface 14 of the culture medium 12 is infected with bacteria which are to be used in the culture. Then the lid 13 is removed from the container 29 and applied. p5. the shells 11 by sliding the flange 21. The flange 18 and the lid are not moved from the layer shown in Fig. 3. If the lid is applied to the steering wheel in relation to the shells, the projections 26 will be inserted into the recesses 27, whereby the disc 20 of the lid will abut against the flange 18 of the outer air shell with the metaholic blanks 15 in contact with the surface of the culture medium. As shown in Fig. 5. If the lid is fitted incorrectly, protruding 26 not abut the lower part of the flange of the lid and limit the displacement of the lid down to a layer in which the aumene are at a distance from the culture medium.

Nar locket 13 och skalen, 11 sammanfOras riktigt satt, sasom visas pa fig. 5, komma rOren 22 och amnena 15 pa. dessa att befinna_ sig mittfor mitten av grupperna 16 av effektivitetszoner, sasoni visas pa. fig. 7. pa grund av donna overensstammelse kan en mindre rutinerad tekniker iaktta.ga och jamfora verkningarna av de olika amnena pa bakterietillvaxten. For att teknikern latt och snabbt skall kunna identifiera dot sarskilda metaboliska amne, som Sr forenat med och befinner sig milli& varje grupp 16 air effektivitetszoner, har gruppen i den i fOrvag fOrpackade enheten, nar de olika airmen som skola anvandas bestamts, markts med heteckningar, sons Oro individuella for det sarskilda medlet. Dessa beteckningar kunna omfatta namnet eller den kemiska synaholen for medlet inristat Morn zongruppen medelst ett ritsverktyg. Beteckningarna ha i delta fall visats soul de stora bokstaverna A. till K. When the lid 13 and the shells 11 are properly assembled, as shown in Fig. 5, the tubes 22 and the blanks 15 come on. these are located in the middle of the groups 16 of efficiency zones, sasoni is shown on. Fig. 7. Due to this agreement, a less experienced technician can observe and compare the effects of the various substances on bacterial growth. In order for the technician to be able to easily and quickly identify the specific metabolic substance, which is associated with and located between each group of 16 air efficiency zones, the group in the pre-packaged unit, when the different airmen to be used have been determined, is marked with hot drawings, sons Concerns individual for the particular means. These designations may include the name or chemical syllable of the agent engraved Morn zone group by means of a drawing tool. The designations have in delta cases shown soul the capital letters A. to K.

Apparaten enlig uppfinningen kan awn anvandas for unclersOkning av sammansaltningen av metallegeringar, dvs, for bestamning av vilka metaller .som fareligga i en okand legering. Vid genomfOrandet av dessa prov lanbringas en losnin.g innehallande delar av den okanda legeringen pa skalens bottenskiva 17 oiler pa, agarskiktet 12. Darefter an,- bringas, sa kallade indikatorer ph de nedre darna air roren 22 pa samma salt som de oven omtalade amnena 15. Indikatorerna reagera darefter olika med lasningen, sa att det blir mojlig att bestamma vilka metaller som foreligga i den senare. The apparatus according to the invention can be used to increase the aggregation of metal alloys, i.e., to determine which metals are hazardous in an unknown alloy. In carrying out these tests, a solution containing parts of the unknown alloy is applied to the bottom plate 17 of the shells on the agar layer 12. Then, so-called indicators of the lower tubes 22 are applied to the same salt as the above-mentioned substances. 15. The indicators then react differently with the welding, so that it becomes possible to determine which metals are present in the latter.

Claims

Patent claim:

1. Combination intended for the study of the effects of air metabolic substances p5. bacterial organisms, comprising a shell (11) having a shell. batten. (17) thanking layer (12) of nutrient culture medium and one to the shells. (11) fitting lid (13), which pa. inside Or provided with protrusions (22), can be characterized by the fact that .portions (15) of the metabolic amrten in solid state Oro supported by nal: Linda protrusions (22) pr5. such salt that they are brought into contact with the nutrient culture medium layer. surface (14) when the lid (13) is attached p5. the scale (11),.

Combination according to claim 1, characterized in that the projections (22) with the metabolic breasts (15) supported therefrom are arranged at a distance from each other in a predetermined sample on the inside of the air cover (13), and that devices (19) are located on . the lid (20) or the shell (17) of the shells, which form groups of distance-marked lines, indicating efficiency zones (16), arranged in a sample corresponding to the monsters of protrusions (22) and darpa applied metabolic Amnon (15), so that the different , the centers of the groups are located opposite the respective subjects.

Combination according to claim 1 or 2, in which the slCalen (11) consists of a generally flat bottom disc (17) with an upwardly directed flange (18) around the circumference and the lid. (13) consists of a generally flat disc (20) of the same shape .as the bottom plate (17) ° eh with a flange (21) projecting from the circumference of the plate (20) .which flanges (18 and 21) can be telescopically displaced in relation to each other, can be characterized in that the flanges (18 and 21) ) arc provided with projections (26) and. recesses (27), which allow fitting the air cover (13) on the shells (11) .only in a predetermined position and prevent assembly of them in the other layers.

A combination according to claims 2 and 3, characterized by designations on an air disks (17 or 20) for identifying each and every one of the groups of zones (16) with the centers of these existing metabolic substances (15).

A combination according to any one of claims 1-1, characterized by a hermetically sealed container (28) enclosing the shells (11) and the lid (13), and. an organ (30) Mom container, which hermetically separates the culture medium (12) Wm the metabolic anmena (15); in order for the device to be prepared, after removal of the organ (30) and infection of the culture medium with bacteria • to be used for examination of the effect of the metabolic substances on the bacteria. Request publications: Patentskrifter! Ran Norge 92507; USA 2,533,089, 2,677,647, 2,694,033.