SE0401626L - A method for establishing a pluripotent human stem cell line derived from blastocytes - Google Patents

A method for establishing a pluripotent human stem cell line derived from blastocytes

Info

Publication number
SE0401626L
SE0401626L SE0401626A SE0401626A SE0401626L SE 0401626 L SE0401626 L SE 0401626L SE 0401626 A SE0401626 A SE 0401626A SE 0401626 A SE0401626 A SE 0401626A SE 0401626 L SE0401626 L SE 0401626L
Authority
SE
Sweden
Prior art keywords
cell line
blastocyst
stem cell
cells
pluripotent human
Prior art date
Application number
SE0401626A
Other languages
Swedish (sv)
Other versions
SE0401626D0 (en
Inventor
Henrik Semb
Anna Tonning
Original Assignee
Cellartis Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0104471A external-priority patent/SE0104471D0/en
Priority claimed from PCT/EP2002/014895 external-priority patent/WO2003055992A2/en
Application filed by Cellartis Ab filed Critical Cellartis Ab
Priority to SE0401626A priority Critical patent/SE0401626L/en
Publication of SE0401626D0 publication Critical patent/SE0401626D0/en
Publication of SE0401626L publication Critical patent/SE0401626L/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/166—Animal cells resulting from interspecies fusion

Abstract

Novel method for obtaining a pluripotent human blastocyst-derived stem cell line. - Obtaining a pluripotent human blastocyst-derived stem cell line comprises steps (i-v): - (i) using a fertilized oocyte optionally, having a grade 1 or 2, to obtain a blastocyst, optionally having a grade A or B; - (ii) co-culturing the blastocyst with feeder cells for establishing one or more colonies of inner cell mass cells; - (iii) isolating the inner cell mass cells by mechanical dissection; - (iv) co-culturing the inner cell mass cells with feeder cells to obtain a blastocyst-derived stem cell line; and optionally - (v) propagating the blastocyst-derived stem cell line. - Independent claims are also included for the following: - (1) a preparation of differentiated stem cells; - (2) producing a pure preparation of insulin-producing differentiated stem cells; and - (3) a kit for producing a preparation of pluripotent human blastocyst-derived stem cell line or insulin-producing differentiated stem cells. - ACTIVITY - Cytostatic; Neuroprotective; Antidiabetic; Antiparkinsonian; Nootropic. No biological data given. - MECHANISM OF ACTION - Gene therapy.
SE0401626A 2001-12-28 2004-06-23 A method for establishing a pluripotent human stem cell line derived from blastocytes SE0401626L (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SE0401626A SE0401626L (en) 2001-12-28 2004-06-23 A method for establishing a pluripotent human stem cell line derived from blastocytes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34277101P 2001-12-28 2001-12-28
SE0104471A SE0104471D0 (en) 2001-12-28 2001-12-28 New method
PCT/EP2002/014895 WO2003055992A2 (en) 2001-12-28 2002-12-27 A method for the establishment of a pluripotent human blastocyst-derived stem cell line
SE0401626A SE0401626L (en) 2001-12-28 2004-06-23 A method for establishing a pluripotent human stem cell line derived from blastocytes

Publications (2)

Publication Number Publication Date
SE0401626D0 SE0401626D0 (en) 2004-06-23
SE0401626L true SE0401626L (en) 2004-08-25

Family

ID=32912859

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0401626A SE0401626L (en) 2001-12-28 2004-06-23 A method for establishing a pluripotent human stem cell line derived from blastocytes

Country Status (1)

Country Link
SE (1) SE0401626L (en)

Also Published As

Publication number Publication date
SE0401626D0 (en) 2004-06-23

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Legal Events

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