SE0400398L - Method to avoid fading when applying fluorescence correlation spectroscopy - Google Patents

Method to avoid fading when applying fluorescence correlation spectroscopy

Info

Publication number
SE0400398L
SE0400398L SE0400398A SE0400398A SE0400398L SE 0400398 L SE0400398 L SE 0400398L SE 0400398 A SE0400398 A SE 0400398A SE 0400398 A SE0400398 A SE 0400398A SE 0400398 L SE0400398 L SE 0400398L
Authority
SE
Sweden
Prior art keywords
correlation spectroscopy
fluorescence correlation
avoid fading
applying fluorescence
applying
Prior art date
Application number
SE0400398A
Other languages
Swedish (sv)
Other versions
SE526643C2 (en
SE0400398D0 (en
Inventor
Anders Hedqvist
Torleif Haerd
Original Assignee
Anders Hedqvist
Torleif Haerd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anders Hedqvist, Torleif Haerd filed Critical Anders Hedqvist
Priority to SE0400398A priority Critical patent/SE526643C2/en
Publication of SE0400398D0 publication Critical patent/SE0400398D0/en
Priority to PCT/SE2005/000223 priority patent/WO2005080945A2/en
Publication of SE0400398L publication Critical patent/SE0400398L/en
Publication of SE526643C2 publication Critical patent/SE526643C2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
SE0400398A 2004-02-20 2004-02-20 Method to avoid bleaching in the application of fluorescence correlation spectroscopy SE526643C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE0400398A SE526643C2 (en) 2004-02-20 2004-02-20 Method to avoid bleaching in the application of fluorescence correlation spectroscopy
PCT/SE2005/000223 WO2005080945A2 (en) 2004-02-20 2005-02-18 Method for avoiding bleaching when using fluorescence correlation spectroscopy

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0400398A SE526643C2 (en) 2004-02-20 2004-02-20 Method to avoid bleaching in the application of fluorescence correlation spectroscopy

Publications (3)

Publication Number Publication Date
SE0400398D0 SE0400398D0 (en) 2004-02-20
SE0400398L true SE0400398L (en) 2005-08-21
SE526643C2 SE526643C2 (en) 2005-10-18

Family

ID=31974271

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0400398A SE526643C2 (en) 2004-02-20 2004-02-20 Method to avoid bleaching in the application of fluorescence correlation spectroscopy

Country Status (2)

Country Link
SE (1) SE526643C2 (en)
WO (1) WO2005080945A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101821608B (en) 2007-07-27 2012-08-08 皇家飞利浦电子股份有限公司 Method and system for imaging a sample
WO2009089056A1 (en) * 2008-01-10 2009-07-16 Pacific Biosciences Of California, Inc. Methods and systems for analysis of fluorescent reactions with modulated excitation
EP4378584A3 (en) * 2010-02-19 2024-11-13 Pacific Biosciences Of California, Inc. Integrated analytical system and method for fluorescence measurement
DE102022208445A1 (en) * 2022-08-15 2024-02-15 Carl Zeiss Microscopy Gmbh Improved FCS procedure

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020167665A1 (en) * 2000-05-19 2002-11-14 Yeung Edward S. High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein
AU2002230592A1 (en) * 2000-10-27 2002-05-06 Molecular Devices Corporation Light detection device
DE10211321A1 (en) * 2002-03-14 2003-09-25 Gnothis Holding Sa Ecublens Use of capture probes for the detection of nucleic acids

Also Published As

Publication number Publication date
WO2005080945A9 (en) 2005-11-03
SE526643C2 (en) 2005-10-18
SE0400398D0 (en) 2004-02-20
WO2005080945A2 (en) 2005-09-01
WO2005080945A3 (en) 2005-12-15

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Legal Events

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