SE0202454D0 - Method and apparatus - Google Patents

Method and apparatus

Info

Publication number
SE0202454D0
SE0202454D0 SE0202454A SE0202454A SE0202454D0 SE 0202454 D0 SE0202454 D0 SE 0202454D0 SE 0202454 A SE0202454 A SE 0202454A SE 0202454 A SE0202454 A SE 0202454A SE 0202454 D0 SE0202454 D0 SE 0202454D0
Authority
SE
Sweden
Prior art keywords
time
alpha2mt
concentration
substrate
product
Prior art date
Application number
SE0202454A
Other languages
Swedish (sv)
Inventor
Goeran Hansson
Original Assignee
Astrazeneca Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astrazeneca Ab filed Critical Astrazeneca Ab
Priority to SE0202454A priority Critical patent/SE0202454D0/en
Publication of SE0202454D0 publication Critical patent/SE0202454D0/en
Priority to PCT/SE2003/001273 priority patent/WO2004016807A1/en
Priority to AU2003248589A priority patent/AU2003248589A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Neurosurgery (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for determining concentration of free thrombin in a sample as a function of time uses a substrate that reacts with thrombin to form a product. A physical property of the product, such as optical absorbance or fluorescence emission, is measured as a function of time. A time derivative of the property is computed, preferably using a time averaging technique, to determine a rate of reaction from substrate to product. The time derivative is then used to determine the concentration of alpha2MT as a function of time and the time derivative of the concentration of alpha2MT used to determine a concentration of free thrombin. The computation minimises the effects of variability in the alpha2MT -dependent decay parameter, k and the parameter kcat2 expressing a rate of turnover of substrate by alpha2MT.
SE0202454A 2002-08-16 2002-08-16 Method and apparatus SE0202454D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE0202454A SE0202454D0 (en) 2002-08-16 2002-08-16 Method and apparatus
PCT/SE2003/001273 WO2004016807A1 (en) 2002-08-16 2003-08-13 Method for determining free thrombin concentration
AU2003248589A AU2003248589A1 (en) 2002-08-16 2003-08-13 Method for determining free thrombin concentration

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0202454A SE0202454D0 (en) 2002-08-16 2002-08-16 Method and apparatus

Publications (1)

Publication Number Publication Date
SE0202454D0 true SE0202454D0 (en) 2002-08-16

Family

ID=20288745

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0202454A SE0202454D0 (en) 2002-08-16 2002-08-16 Method and apparatus

Country Status (3)

Country Link
AU (1) AU2003248589A1 (en)
SE (1) SE0202454D0 (en)
WO (1) WO2004016807A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004059055A1 (en) 2004-12-07 2006-06-08 Dade Behring Marburg Gmbh Method for automatically determining endogenous thrombin potential
US9091700B2 (en) 2005-12-27 2015-07-28 Mcmaster University Modified peptide substrate
JP5212821B2 (en) 2005-12-27 2013-06-19 マクマスター ユニバーシティー Enzyme assay using a modified substrate containing a substrate that binds to a polymer via a spacer
DE102006035899A1 (en) 2006-07-31 2008-02-07 Dade Behring Marburg Gmbh New polysaccharide-peptide-conjugate, where the peptide portion contains a sequence having a signal group that is splittable by thrombin at its carbon-terminal end, useful in a procedure for determining the thrombin generation in a sample
EP2975383A1 (en) * 2014-07-14 2016-01-20 Johann Wolfgang Goethe-Universität, Frankfurt am Main Method and device for analyzing a fluid medium such as a blood sample
ES2911600T3 (en) 2017-06-20 2022-05-20 Inst Nat Sante Rech Med Methods to identify if patients with acute decompensated heart failure (ADHF) have a hypercoagulable state

Also Published As

Publication number Publication date
AU2003248589A1 (en) 2004-03-03
WO2004016807A1 (en) 2004-02-26

Similar Documents

Publication Publication Date Title
Luz et al. The isotopic ratios 17O/16O and 18O/16O in molecular oxygen and their significance in biogeochemistry
ATE310101T1 (en) METHOD AND KIT FOR DETECTING KINASE ACTIVITY
Hönisch et al. Assessing scleractinian corals as recorders for paleo-pH: Empirical calibration and vital effects
Martz et al. A submersible autonomous sensor for spectrophotometric pH measurements of natural waters
ATE488604T1 (en) POLYNUCLEOTIDE DETECTION SYSTEM
ATE431427T1 (en) IMPROVED METHOD FOR DETECTING ATP
WO2006023725A3 (en) FLUORESCENT pH DETECTOR SYSTEM AND RELATED METHODS
NO20074726L (en) Iconic display of markers for a painter
SE9903192D0 (en) Procedure for determination of gas content
Iacovino Linking subsurface to surface degassing at active volcanoes: A thermodynamic model with applications to Erebus volcano
SE0202454D0 (en) Method and apparatus
Zhou et al. Frequent carbon input primes decomposition of decadal soil organic matter
ATE371015T1 (en) IMPROVEMENT IN A METHOD FOR QUANTIFYING BACTERIAL ENDOSPORES USING LANTHANIDE DIPICOLINATE LUMINESCENCE
Chen et al. A high-temperature catalytic oxidation method for the determination of marine dissolved organic carbon and its comparison with the UV photo-oxidation method
MXPA03010143A (en) An assay for detecting inhibitors of the enzyme mueloperokidase.
FI955964A0 (en) Determination of toxicity
Provenzale et al. High-frequency productivity estimates for a lake from free-water CO 2 concentration measurements
ATE346951T1 (en) TEST FOR DETECTING INHIBITORS OF THE ENZYME MYELOPEROKIDASE
AR005521A1 (en) METHOD FOR MONITORING A MICROBIOLOGICAL PROCESS IN A SOURCE OF FLUIDS THAT HAS A MICROBIOLOGICAL POPULATION.
Herrmann et al. Comparison of methods to quantify respirational carbon loss of coarse woody debris
DE602006011748D1 (en) Microsensor for the determination of D-amino acids
DE60003314D1 (en) MONITORING HYDROGEN PEROXIDE
CN104380086A (en) Quantitative analyzing method for oxidant and quantitative analyzing instrument for oxidant
Orlando et al. Atmospheric chemistry of hydrazoic acid (HN3): UV absorption spectrum, HO• reaction rate, and reactions of the• N3 radical
Guida et al. Riboflavin (vitamin B2) assay by adsorptive cathodic stripping voltammetry (adcsv) at the hanging mercury drop electrode (HMDE)