SA110310142B1 - Syring for Diagnose of Malaria and Other Blood Diseases - Google Patents

Abstract

Abstract: The invention is a syringe equipped with special filters and a basin in which nitrocellulose paper (separation medium) saturated with separation material is placed. When the blood sample is drawn, it passes through the first filter to filter large particles and white blood cells, and then passes to the second filter to reserve red blood cells and platelets Blood plasma passes after filtration leaving only plasma proteins represented by antigens and antibodies. The plasma reaches the vat and is spread across a nitrocellulose paper impregnated with a separator that separates the proteins from each other. Antibodies specific to the malaria parasite or other pathogen of blood diseases are added to the vat, which are bound to a fluorescent substance that produces a color if the antibody binds to its antigen for that pathogen. .

Description

Y — Syring for Diagnose of Malaria and Other Blood Diseases Full Description Background of the selection Malaria is diagnosed by a simple method of diagnosis whereby a blood sample is drawn from the patient with a syringe into an EDTA tube (to prevent clotting) (pal) and then put it on a glass slide and examine it by means of a microscope, but after the examination, the problem of getting rid of the syringe, slide, and tube appears, which often contains an amount of all, and therefore the disposal of these residues in a safe way through special containers It is considered a costly, long and complicated process General description of the invention Needle parts: 4. The blood sample passes through the malaria diagnostic needle in several parts: 1- The tip of the needle (1), which is disposed die after each diagnosis and includes the tip of the needle (1a) and the base of the needle tip containing EDTA sale (1AP) “- the large filter (©) that separates large particles from the blood sample and is also replaced after each diagnosis vo” - the small filter (£) for small particles of onion The blood sample is also replaced after each diagnosis 4- The main stream (7) and nitrocellulose paper is placed in it (1) and the paper is replaced after every 7 This method is considered one of the simplest methods for diagnosing malaria and other blood diseases, as a blood sample is drawn from the patient Rally Through the changeable head (1) BY) and pass from the tip of the needle (11) to the base of the needle tip containing EDTA (1b), a substance widely used as a substance that preserves blood flow 0 pass a sample blood through the large filter (9) and it is filtered

Y — — pore size ranges from 01-17 μm’ and separates blood cells (Land (Y0=VY) granulocytes μm); monocytes (YA=YY µm); And large lymphocytes (0 micrometers); after that, the rest of the blood sample passes through: the small filter (4) whose holes size ranges from T= micrometers and filters red blood cells (V) Erythrocytes micrometers) » Small lymphocytes (0 μm) and blood platelets (? μm). After filtration, blood plasma remains, which reaches the immersion basin (0) and migrates through nitrocellulose paper (1) Cus, the dimensions of which do not exceed © cm, which is saturated with separation material (a substance that separates plasma proteins “Sodium dodecyl sale Jie” (sulphate) after that. Anti-malarial or other anti-pathogen antibodies are added

0 enzyme linked antibodies that have the ability to bind to the antigen of the malaria parasite or other pathogens (if any). A chromogenic substrate that binds to the enzyme and then causes a visible (V) color change if the pathogen is present in the blood sample being tested.

10 The remains of the needle after the completion of the examination are the needle tip (1); the large filter (7), the small filter (;), and the nitrocellulose paper (1) only, and these can be used by single these large filter (©) and the small filter (4 ) on glass slides and examined separately under a microscope to detect any changes in blood cells, whether in shape or adie. After that, these dry residues can be disposed of in an easy and safe way. The results of this examination method are considered fast as the time required for the result to appear

© represented in the speed of diffusion through the nitrocellulose paper (7). The idea could be applied to any parasite or other microbe that attacks human blood. Brief explanation of the drawings: Figure (1): General view of the needle from the top with the filter paper perpendicular to the needle Figure (7): General view of the needle from the side with the filter paper parallel HM

ve Figure (7): Needle head and large filter Figure (?): Needle head, large filter and small filter Figure (0) Needle head, large filter, small filter and filter paper

Squirt

Figure (1) Needle mechanism Detailed description: It is clear from the attached drawings Figure (1) and (1) the parts of the needle, and the nitrocellulose paper (1) can be placed perpendicular to the needle axis Figure (1) or parallel to the needle axis Fig. oY) Parts start at 0 needle head (1) replaceable Fig. (1=T) which contains needle thread (1a) Fig. (©3©-1a) and needle thread base (1b) Figure (@V=T) which is Ble about a thin tube containing drops of EDTA liquid for blood clotting; followed by the main channel (1) of the needle Figure (7-7) and then the large filter (¥) ( The diameter of its holes is 17-01 μm) that traps large blood cells (granulocytes; monocytes and lymphocytes) shaped (T=) after the large filter there is the small 0 filter (4) (1 cup diameter -? micrometer) and filters red blood cells, small lymphocytes, and platelets (Fig. (?-4). After that, a nitrocellulose sheet (1) (Fig. (*-1) is placed so that its lower end is in contact with the immersion basin (0) (Fig. (0-0) 0) In which blood plasma is drawn after filtering and enzyme-linked antibodies and any other substances are placed in it to migrate through nitrocellulose paper (1) and give a color change (Y) Figure (7-7) when disease is present. The last part of the needle is the rear 1s piston (A) Fig. (4-3) which withdraws a pall sample from the infected person and then pulls the sample through the large filter (?) and the small filter (4) until it reaches a paper nitrocellulose (1). F

Claims (1)

__ M _- Protection elements 1-1 innovative needle that separates li Se blood and diagnoses disease at the same time. 17 1 innovative needle, as in protection element 1, works to draw blood, and contains special 0 filters with a diameter of 10-18 micrometers. 1 © needle as in the protection element ¥ contains special filters with holes 101 0 La lad Y micrometers for blood purification and diagnosis of blood diseases. 1?; Needle, as in the protective element, Y, containing special filters with holes of diameter, Yo, YY, La, micrometer, for blood purification and diagnosis of blood diseases. 1J- A needle as in the protection element 1 containing special filters with holes of YA SY Y micrometer diameter for blood purification and diagnosis of blood diseases. +101 needle as in protection 1 is used to diagnose malaria and some other diseases in one easy and safe step. 1 +” _ Needle as in protection element 1 used to withdraw a small amount of blood for the purpose of diagnosing 7 malaria ang infectious blood diseases and thus does not result in pathological residues: 0 difficult to deal with.