RU99127946A - METHOD FOR PRODUCING IMMUNO-FLUORESCING ANTIBODIES FOR DETECTING THE TOXOPLASMOSIS PATTERN - Google Patents

METHOD FOR PRODUCING IMMUNO-FLUORESCING ANTIBODIES FOR DETECTING THE TOXOPLASMOSIS PATTERN

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Publication number
RU99127946A
RU99127946A RU99127946/14A RU99127946A RU99127946A RU 99127946 A RU99127946 A RU 99127946A RU 99127946/14 A RU99127946/14 A RU 99127946/14A RU 99127946 A RU99127946 A RU 99127946A RU 99127946 A RU99127946 A RU 99127946A
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RU
Russia
Prior art keywords
toxoplasmosis
detecting
pattern
producing
antibodies
Prior art date
Application number
RU99127946/14A
Other languages
Russian (ru)
Other versions
RU2174233C1 (en
Inventor
Тамара Петровна Пашанина
Галина Матвеевна Напалкова
Виктор Васильевич Мананков
Original Assignee
Волгоградский научно-исследовательский противочумный институт
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Application filed by Волгоградский научно-исследовательский противочумный институт filed Critical Волгоградский научно-исследовательский противочумный институт
Publication of RU99127946A publication Critical patent/RU99127946A/en
Application granted granted Critical
Publication of RU2174233C1 publication Critical patent/RU2174233C1/en

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Claims (1)

Способ получения иммунофлуоресцирующих антител для обнаружения возбудителя токсоплазмоза, включающий выделение из сыворотки иммуноглобулинов, отличающийся тем, что сыворотку получают на 10 - 14 сут обострения болезни от больных хронической формой токсоплазмоза, болеющих на протяжении 2 - 10 лет, в количестве 5 - 10 мл, с титрами антител в НМФА ≥ 1 : 640 или в ИФА ≥ 1 : 3200, инактивируют при 56oС в течение 30 мин, после чего выделяют фракцию иммуноглобулинов двухкратным осаждением 20% раствором полиэтиленгликоля, тип 6000, и метят флуоресцеин-5-изотиоцианатом в соотношении Igтокс-ФИТЦ - 20 : 1.A method of producing immunofluorescent antibodies for detecting the causative agent of toxoplasmosis, including the isolation of serum immunoglobulins, characterized in that the serum is obtained on 10-14 days of exacerbation of the disease from patients with chronic toxoplasmosis who have been ill for 2-10 years, in the amount of 5-10 ml, s antibody titers in NMFA ≥ 1: 640 in ELISA or ≥ 1: 3200, inactivated at 56 o C for 30 minutes, after which the immunoglobulin fraction was isolated by precipitation of the double solution of 20% polyethylene glycol type 6000, and labeled with fluorescein-5-isothiocyano- om in relation Igtoks-FITC - 20: 1.
RU99127946A 1999-12-30 Method for producing immunofluorescent antibodies for detecting toxoplasmosis pathogen RU2174233C1 (en)

Publications (2)

Publication Number Publication Date
RU99127946A true RU99127946A (en) 2001-09-10
RU2174233C1 RU2174233C1 (en) 2001-09-27

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