RU99106754A - METHOD FOR PRODUCING POLYEPEPTIDES IN A CELLLESS SYSTEM - Google Patents

METHOD FOR PRODUCING POLYEPEPTIDES IN A CELLLESS SYSTEM

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Publication number
RU99106754A
RU99106754A RU99106754/13A RU99106754A RU99106754A RU 99106754 A RU99106754 A RU 99106754A RU 99106754/13 A RU99106754/13 A RU 99106754/13A RU 99106754 A RU99106754 A RU 99106754A RU 99106754 A RU99106754 A RU 99106754A
Authority
RU
Russia
Prior art keywords
synthesis
mixture
reaction mixture
components
combinations
Prior art date
Application number
RU99106754/13A
Other languages
Russian (ru)
Other versions
RU2169154C2 (en
Inventor
Сергей Владимирович Бирюков
Петр Николаевич Симоненко
Владимир Анатольевич Широков
Александр Сергеевич Спирин
Original Assignee
Институт белка Российской академии наук
Filing date
Publication date
Application filed by Институт белка Российской академии наук filed Critical Институт белка Российской академии наук
Priority claimed from RU99106754/13A external-priority patent/RU2169154C2/en
Priority to RU99106754/13A priority Critical patent/RU2169154C2/en
Priority to US09/937,474 priority patent/US6783957B1/en
Priority to DE60032227T priority patent/DE60032227T2/en
Priority to PCT/EP2000/002508 priority patent/WO2000058493A1/en
Priority to ES00912629T priority patent/ES2277832T3/en
Priority to AT00912629T priority patent/ATE347610T1/en
Priority to CA002365237A priority patent/CA2365237C/en
Priority to JP2000608772A priority patent/JP3836679B2/en
Priority to EP00912629A priority patent/EP1165826B1/en
Publication of RU99106754A publication Critical patent/RU99106754A/en
Publication of RU2169154C2 publication Critical patent/RU2169154C2/en
Application granted granted Critical

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Claims (9)

1. Метод синтеза полипептидов в бесклеточной системе, по которому приготавливают реакционную смесь, используя клеточный лизат или экстракт, выбирают режим синтеза и область значений, в которой устанавливают параметры бесклеточной системы и параметры процесса синтеза, определяют способ обмена компонентами между реакционной смесью и питающей смесью, выбирают тип и параметры, по крайней мере одного, пористого барьера, вводят реакционную смесь и питающую смесь в реакционный модуль и проводят синтез, отличающийся тем, что на этапе выбора параметров процесса определяют типы выбранных компонентов, которые влияют на эффективность синтеза, определяют верхнюю и нижнюю границы диапазона, в котором в процессе синтеза изменяют значения концентраций выбранных компонентов, формируют дополнительную смесь, в состав которой входят выбранные компоненты, вводят дополнительную смесь в реакционную смесь или в питающую смесь, проводят синтез в условиях, когда концентрации выбранных компонентов изменяют в выбранном диапазоне, а концентрации других компонентов поддерживают постоянными.1. The method of synthesis of polypeptides in a cell-free system, according to which the reaction mixture is prepared using a cell lysate or extract, selects the synthesis mode and the range of values in which the parameters of the cell-free system and the parameters of the synthesis process are set, determine the method of exchange of components between the reaction mixture and the feed mixture, the type and parameters of at least one porous barrier are selected, the reaction mixture and the feed mixture are introduced into the reaction module, and synthesis is carried out, characterized in that at the stage of steam selection process meters determine the types of selected components that affect the synthesis efficiency, determine the upper and lower boundaries of the range in which the concentration values of the selected components are changed during the synthesis process, form an additional mixture, which includes selected components, add an additional mixture to the reaction mixture or the feed mixture, synthesis is carried out under conditions when the concentrations of the selected components are changed in the selected range, and the concentrations of the other components are kept constant. 2. Способ по п.1, отличающийся тем, что по крайней мере один из выбранных компонентов входит в состав группы, состоящей из Mg+2, К+, NТР, polyamine или их комбинаций.2. The method according to claim 1, characterized in that at least one of the selected components is included in the group consisting of Mg +2 , K + , NTP, polyamine, or combinations thereof. 3. Способ по п. 2, отличающийся тем, что в состав одной из комбинаций выбранных компонентов входят Mg+2 и NТР.3. The method according to p. 2, characterized in that the composition of one of the combinations of the selected components includes Mg +2 and NTP. 4. Способ по п.1, отличающийся тем, что способ синтеза выбирают по крайней мере из одного режима, выбираемого из группы включающей: трансляцию, сопряженную транскрипцию - трансляцию, транскрипцию - трансляцию проводимых в одном объеме, транскрипцию или из комбинаций этих режимов. 4. The method according to claim 1, characterized in that the synthesis method is selected from at least one mode selected from the group including: translation, conjugated transcription - translation, transcription - translation carried out in one volume, transcription or from combinations of these modes. 5. Способ по п.4, отличающийся тем, что, в зависимости от режима синтеза, NТР, которые включены в состав дополнительной смеси, состоят из группы АТР, GТР, UТР, СТР или АТР, GТР. 5. The method according to claim 4, characterized in that, depending on the synthesis mode, NTP, which are included in the additional mixture, consist of the group ATP, GTP, UTP, CTP or ATP, GTP. 6. Способ по п.1, отличающийся тем, что дополнительную смесь вводят в реакционную смесь до синтеза или во время синтеза, или дополнительную смесь вводят в часть питающей смеси до синтеза или во время синтеза. 6. The method according to claim 1, characterized in that the additional mixture is introduced into the reaction mixture before synthesis or during synthesis, or the additional mixture is introduced into part of the feed mixture before synthesis or during synthesis. 7. Способ по п. 6, отличающийся тем, что ввод дополнительной смеси во время синтеза осуществляют однократно, периодически или непрерывно. 7. The method according to p. 6, characterized in that the introduction of the additional mixture during the synthesis is carried out once, periodically or continuously. 8. Способ по п.1, отличающийся тем, что ввод низкомолекулярных компонентов питающей смеси в реакционную смесь выбирают из группы режимов непрерывного обмена или группы режимов непрерывного потока или комбинаций этих режимов. 8. The method according to claim 1, characterized in that the introduction of low molecular weight components of the feed mixture into the reaction mixture is selected from the group of continuous exchange modes or the group of continuous flow modes or combinations of these modes. 9. Способ по п.1, отличающийся тем, что реакционную смесь приготавливают на основе бесклеточной системы которую приготавливают из клеток прокариотов или клеток эукариотов или их комбинаций. 9. The method according to claim 1, characterized in that the reaction mixture is prepared on the basis of a cell-free system which is prepared from prokaryotic cells or eukaryotic cells, or combinations thereof.
RU99106754/13A 1999-03-25 1999-03-25 Method of synthesis of polypeptides in cell-free system RU2169154C2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
RU99106754/13A RU2169154C2 (en) 1999-03-25 1999-03-25 Method of synthesis of polypeptides in cell-free system
ES00912629T ES2277832T3 (en) 1999-03-25 2000-03-22 METHOD OF SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS.
DE60032227T DE60032227T2 (en) 1999-03-25 2000-03-22 METHOD FOR SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS
PCT/EP2000/002508 WO2000058493A1 (en) 1999-03-25 2000-03-22 Method for synthesis of polypeptides in cell-free systems
US09/937,474 US6783957B1 (en) 1999-03-25 2000-03-22 Method for synthesis of polypeptides in cell-free systems
AT00912629T ATE347610T1 (en) 1999-03-25 2000-03-22 METHOD FOR SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS
CA002365237A CA2365237C (en) 1999-03-25 2000-03-22 Method for synthesis of polypeptides in cell-free systems
JP2000608772A JP3836679B2 (en) 1999-03-25 2000-03-22 Method for synthesizing polypeptide in cell-free system
EP00912629A EP1165826B1 (en) 1999-03-25 2000-03-22 Method for synthesis of polypeptides in cell-free systems

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU99106754/13A RU2169154C2 (en) 1999-03-25 1999-03-25 Method of synthesis of polypeptides in cell-free system

Publications (2)

Publication Number Publication Date
RU99106754A true RU99106754A (en) 2001-01-20
RU2169154C2 RU2169154C2 (en) 2001-06-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU99106754/13A RU2169154C2 (en) 1999-03-25 1999-03-25 Method of synthesis of polypeptides in cell-free system

Country Status (9)

Country Link
US (1) US6783957B1 (en)
EP (1) EP1165826B1 (en)
JP (1) JP3836679B2 (en)
AT (1) ATE347610T1 (en)
CA (1) CA2365237C (en)
DE (1) DE60032227T2 (en)
ES (1) ES2277832T3 (en)
RU (1) RU2169154C2 (en)
WO (1) WO2000058493A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10011209A1 (en) * 2000-03-08 2001-09-13 Roche Diagnostics Gmbh Reactor for reacting low- and high-molecular components, especially for coupled in vitro transcription-translation protein preparation reactions, including reactor chamber containing swellable matrix
DE10137792A1 (en) * 2001-08-06 2003-02-27 Erdmann Volker Method of gene expression
EP1295894B1 (en) 2001-09-25 2011-09-14 F. Hoffmann-La Roche AG Method for an in vitro sequence specific biotinylation of polypeptides
CA2463719A1 (en) 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
US7341852B2 (en) * 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
JPWO2005123936A1 (en) * 2004-06-15 2008-04-10 三菱化学株式会社 Polypeptide synthesis method
JP4513495B2 (en) 2004-10-15 2010-07-28 株式会社島津製作所 Cell-free protein synthesis method using insect cell-derived extract
US20080248565A1 (en) * 2007-03-01 2008-10-09 Invitrogen Corporation Isolated phospholipid-protein particles
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
US9399670B2 (en) 2009-02-20 2016-07-26 Hoffmann-La Roche Inc. Method for obtaining immunoglobulin encoding nucleic acid
BR112014004566A2 (en) 2011-09-21 2017-06-13 Hoffmann La Roche IgG cognate light and heavy chain amplification and quantification method, monoclonal antibody production method, immunoglobulin and immunoglobulin fab fragment production methods, nucleic acid and kit
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
KR20230048162A (en) 2015-03-30 2023-04-10 그린라이트 바이오사이언시스, 아이엔씨. Cell-free production of ribonucleic acid
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
RU199182U1 (en) * 2019-12-10 2020-08-19 Федеральное государственное бюджетное учреждение науки Институт белка Российской академии наук (ИБ РАН) DEVICE FOR PROTEIN SYNTHESIS IN A CELLLESS SYSTEM

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668624A (en) 1979-02-28 1987-05-26 E.I. Du Pont De Nemours And Company Protein translation method
US5135853A (en) 1983-07-22 1992-08-04 Rensselaer Polytechnic Institute Three compartment bioreactor and method of use
SU1618761A1 (en) 1987-04-29 1991-01-07 Институт Белка Ан Ссср Method of producing peptides and proteins in cell-less translation system
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
SU1705302A1 (en) 1988-12-22 1992-01-15 Институт Белка Ан Ссср Method of preparative genes expression in the cell-free system of coupled transcription/translation
US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
DE3914956A1 (en) 1989-05-06 1990-11-22 Fischer Karl Heinz METHOD FOR ACCELERATING THE MATERIAL REPLACEMENT OF A CONTINUOUS BIOREACTOR AND DEVICE FOR CARRYING OUT THIS METHOD
ATE130633T1 (en) 1989-07-31 1995-12-15 Inst Belka Akad Nauk Sssr METHOD FOR PRODUCING POLYPEPTIDES IN A CELL-FREE TRANSLATION SYSTEM.
CA2064754C (en) 1989-07-31 1996-06-18 Vladimir Ivanovich Baranov Method of preparing polypeptides in a cell-free translation system
CA2115991A1 (en) 1991-06-24 1993-01-07 Eckard Wimmer De novo cell-free synthesis of picornavirus
JPH0576381A (en) 1991-09-19 1993-03-30 Hitachi Ltd Method for synthesizing polypeptide
DE69216385T2 (en) 1991-10-11 1997-06-12 Promega Corp COUPLED TRANSCRIPTION AND TRANSLATION IN CELL-FREE EUKARYOT EXTRACTS
WO1994005812A1 (en) 1992-09-02 1994-03-17 The Scripps Research Institute Coupled isothermal polynucleotide amplification and translation system
BR9300343A (en) 1993-02-09 1994-09-27 Semer S A Multifunction dryer
US5434079A (en) 1993-02-12 1995-07-18 The United States Of America As Represented By The Department Of Health And Human Services Apparatus and process for continuous in vitro synthesis of proteins
JPH0731494A (en) 1993-07-14 1995-02-03 Hitachi Ltd Method for production protein in cell-free system and device therefor
US5324428A (en) 1993-07-19 1994-06-28 Spectrum Medical Industries, Inc. Disposable dialysis apparatus
JPH0775592A (en) 1993-09-07 1995-03-20 Hitachi Ltd Method for synthesizing protein by extracted solution of cell and device therefor
EP0720508B1 (en) 1993-09-22 2000-04-05 Pierce Chemical Company Device for dialyzing samples
KR0131166B1 (en) 1994-05-04 1998-04-11 최차용 Preparation process of proteins in a non-cell system
JPH1080295A (en) 1996-07-15 1998-03-31 Nippon Flour Mills Co Ltd Synthesis of protein by cell-free protein synthetic system and apparatus therefor
DE59611424D1 (en) 1996-07-16 2007-05-03 Qiagen North American Holdings METHOD FOR PRODUCING COMPLEX MULTI-CYMATIC STORAGE-STABLE REACTION MIXTURES AND THEIR USE
DE19832160A1 (en) 1997-07-31 1999-02-04 Boehringer Mannheim Gmbh New peptide synthesis bioreactor
RU2148649C1 (en) 1998-03-31 2000-05-10 Институт белка РАН Method and apparatus for preparing polypeptides in acellular system (versions)

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