RU99106754A - METHOD FOR PRODUCING POLYEPEPTIDES IN A CELLLESS SYSTEM - Google Patents
METHOD FOR PRODUCING POLYEPEPTIDES IN A CELLLESS SYSTEMInfo
- Publication number
- RU99106754A RU99106754A RU99106754/13A RU99106754A RU99106754A RU 99106754 A RU99106754 A RU 99106754A RU 99106754/13 A RU99106754/13 A RU 99106754/13A RU 99106754 A RU99106754 A RU 99106754A RU 99106754 A RU99106754 A RU 99106754A
- Authority
- RU
- Russia
- Prior art keywords
- synthesis
- mixture
- reaction mixture
- components
- combinations
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 claims 17
- 230000015572 biosynthetic process Effects 0.000 claims 13
- 238000003786 synthesis reaction Methods 0.000 claims 13
- 230000002194 synthesizing Effects 0.000 claims 13
- 239000011541 reaction mixture Substances 0.000 claims 7
- 210000004671 Cell-Free System Anatomy 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 230000035897 transcription Effects 0.000 claims 3
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims 2
- XKMLYUALXHKNFT-UUOKFMHZSA-N Guanosine-5'-triphosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XKMLYUALXHKNFT-UUOKFMHZSA-N 0.000 claims 2
- PCDQPRRSZKQHHS-XVFCMESISA-N ({[({[(2R,3S,4R,5R)-5-(4-amino-2-oxo-1,2-dihydropyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)phosphonic acid Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-XVFCMESISA-N 0.000 claims 1
- PGAVKCOVUIYSFO-XVFCMESISA-N Uridine triphosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-XVFCMESISA-N 0.000 claims 1
- 210000004027 cells Anatomy 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 239000006166 lysate Substances 0.000 claims 1
- 229920000768 polyamine Polymers 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 238000001308 synthesis method Methods 0.000 claims 1
- 229950010342 uridine triphosphate Drugs 0.000 claims 1
Claims (9)
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU99106754/13A RU2169154C2 (en) | 1999-03-25 | 1999-03-25 | Method of synthesis of polypeptides in cell-free system |
ES00912629T ES2277832T3 (en) | 1999-03-25 | 2000-03-22 | METHOD OF SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS. |
DE60032227T DE60032227T2 (en) | 1999-03-25 | 2000-03-22 | METHOD FOR SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS |
PCT/EP2000/002508 WO2000058493A1 (en) | 1999-03-25 | 2000-03-22 | Method for synthesis of polypeptides in cell-free systems |
US09/937,474 US6783957B1 (en) | 1999-03-25 | 2000-03-22 | Method for synthesis of polypeptides in cell-free systems |
AT00912629T ATE347610T1 (en) | 1999-03-25 | 2000-03-22 | METHOD FOR SYNTHESIS OF POLYPEPTIDES IN CELL-FREE SYSTEMS |
CA002365237A CA2365237C (en) | 1999-03-25 | 2000-03-22 | Method for synthesis of polypeptides in cell-free systems |
JP2000608772A JP3836679B2 (en) | 1999-03-25 | 2000-03-22 | Method for synthesizing polypeptide in cell-free system |
EP00912629A EP1165826B1 (en) | 1999-03-25 | 2000-03-22 | Method for synthesis of polypeptides in cell-free systems |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU99106754/13A RU2169154C2 (en) | 1999-03-25 | 1999-03-25 | Method of synthesis of polypeptides in cell-free system |
Publications (2)
Publication Number | Publication Date |
---|---|
RU99106754A true RU99106754A (en) | 2001-01-20 |
RU2169154C2 RU2169154C2 (en) | 2001-06-20 |
Family
ID=20217990
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU99106754/13A RU2169154C2 (en) | 1999-03-25 | 1999-03-25 | Method of synthesis of polypeptides in cell-free system |
Country Status (9)
Country | Link |
---|---|
US (1) | US6783957B1 (en) |
EP (1) | EP1165826B1 (en) |
JP (1) | JP3836679B2 (en) |
AT (1) | ATE347610T1 (en) |
CA (1) | CA2365237C (en) |
DE (1) | DE60032227T2 (en) |
ES (1) | ES2277832T3 (en) |
RU (1) | RU2169154C2 (en) |
WO (1) | WO2000058493A1 (en) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10011209A1 (en) * | 2000-03-08 | 2001-09-13 | Roche Diagnostics Gmbh | Reactor for reacting low- and high-molecular components, especially for coupled in vitro transcription-translation protein preparation reactions, including reactor chamber containing swellable matrix |
DE10137792A1 (en) * | 2001-08-06 | 2003-02-27 | Erdmann Volker | Method of gene expression |
EP1295894B1 (en) | 2001-09-25 | 2011-09-14 | F. Hoffmann-La Roche AG | Method for an in vitro sequence specific biotinylation of polypeptides |
CA2463719A1 (en) | 2003-04-05 | 2004-10-05 | F. Hoffmann-La Roche Ag | Nucleotide analogs with six membered rings |
US7341852B2 (en) * | 2003-07-18 | 2008-03-11 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of decoupling reaction scale and protein synthesis yield in batch mode |
JPWO2005123936A1 (en) * | 2004-06-15 | 2008-04-10 | 三菱化学株式会社 | Polypeptide synthesis method |
JP4513495B2 (en) | 2004-10-15 | 2010-07-28 | 株式会社島津製作所 | Cell-free protein synthesis method using insect cell-derived extract |
US20080248565A1 (en) * | 2007-03-01 | 2008-10-09 | Invitrogen Corporation | Isolated phospholipid-protein particles |
US20070117179A1 (en) * | 2005-09-27 | 2007-05-24 | Invitrogen Corporation | In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles |
US9399670B2 (en) | 2009-02-20 | 2016-07-26 | Hoffmann-La Roche Inc. | Method for obtaining immunoglobulin encoding nucleic acid |
BR112014004566A2 (en) | 2011-09-21 | 2017-06-13 | Hoffmann La Roche | IgG cognate light and heavy chain amplification and quantification method, monoclonal antibody production method, immunoglobulin and immunoglobulin fab fragment production methods, nucleic acid and kit |
US9528137B2 (en) | 2013-03-15 | 2016-12-27 | Northwestern University | Methods for cell-free protein synthesis |
KR20230048162A (en) | 2015-03-30 | 2023-04-10 | 그린라이트 바이오사이언시스, 아이엔씨. | Cell-free production of ribonucleic acid |
US11530432B2 (en) | 2018-03-19 | 2022-12-20 | Northwestern University | Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates |
US11725224B2 (en) | 2018-04-16 | 2023-08-15 | Northwestern University | Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates |
WO2020040840A2 (en) | 2018-06-01 | 2020-02-27 | Northwestern University | Expanding the chemical substrates for genetic code reprogramming |
RU199182U1 (en) * | 2019-12-10 | 2020-08-19 | Федеральное государственное бюджетное учреждение науки Институт белка Российской академии наук (ИБ РАН) | DEVICE FOR PROTEIN SYNTHESIS IN A CELLLESS SYSTEM |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4668624A (en) | 1979-02-28 | 1987-05-26 | E.I. Du Pont De Nemours And Company | Protein translation method |
US5135853A (en) | 1983-07-22 | 1992-08-04 | Rensselaer Polytechnic Institute | Three compartment bioreactor and method of use |
SU1618761A1 (en) | 1987-04-29 | 1991-01-07 | Институт Белка Ан Ссср | Method of producing peptides and proteins in cell-less translation system |
US4937190A (en) | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
SU1705302A1 (en) | 1988-12-22 | 1992-01-15 | Институт Белка Ан Ссср | Method of preparative genes expression in the cell-free system of coupled transcription/translation |
US5478730A (en) * | 1988-12-21 | 1995-12-26 | Institute Of Protein Research | Method of preparing polypeptides in cell-free translation system |
DE3914956A1 (en) | 1989-05-06 | 1990-11-22 | Fischer Karl Heinz | METHOD FOR ACCELERATING THE MATERIAL REPLACEMENT OF A CONTINUOUS BIOREACTOR AND DEVICE FOR CARRYING OUT THIS METHOD |
ATE130633T1 (en) | 1989-07-31 | 1995-12-15 | Inst Belka Akad Nauk Sssr | METHOD FOR PRODUCING POLYPEPTIDES IN A CELL-FREE TRANSLATION SYSTEM. |
CA2064754C (en) | 1989-07-31 | 1996-06-18 | Vladimir Ivanovich Baranov | Method of preparing polypeptides in a cell-free translation system |
CA2115991A1 (en) | 1991-06-24 | 1993-01-07 | Eckard Wimmer | De novo cell-free synthesis of picornavirus |
JPH0576381A (en) | 1991-09-19 | 1993-03-30 | Hitachi Ltd | Method for synthesizing polypeptide |
DE69216385T2 (en) | 1991-10-11 | 1997-06-12 | Promega Corp | COUPLED TRANSCRIPTION AND TRANSLATION IN CELL-FREE EUKARYOT EXTRACTS |
WO1994005812A1 (en) | 1992-09-02 | 1994-03-17 | The Scripps Research Institute | Coupled isothermal polynucleotide amplification and translation system |
BR9300343A (en) | 1993-02-09 | 1994-09-27 | Semer S A | Multifunction dryer |
US5434079A (en) | 1993-02-12 | 1995-07-18 | The United States Of America As Represented By The Department Of Health And Human Services | Apparatus and process for continuous in vitro synthesis of proteins |
JPH0731494A (en) | 1993-07-14 | 1995-02-03 | Hitachi Ltd | Method for production protein in cell-free system and device therefor |
US5324428A (en) | 1993-07-19 | 1994-06-28 | Spectrum Medical Industries, Inc. | Disposable dialysis apparatus |
JPH0775592A (en) | 1993-09-07 | 1995-03-20 | Hitachi Ltd | Method for synthesizing protein by extracted solution of cell and device therefor |
EP0720508B1 (en) | 1993-09-22 | 2000-04-05 | Pierce Chemical Company | Device for dialyzing samples |
KR0131166B1 (en) | 1994-05-04 | 1998-04-11 | 최차용 | Preparation process of proteins in a non-cell system |
JPH1080295A (en) | 1996-07-15 | 1998-03-31 | Nippon Flour Mills Co Ltd | Synthesis of protein by cell-free protein synthetic system and apparatus therefor |
DE59611424D1 (en) | 1996-07-16 | 2007-05-03 | Qiagen North American Holdings | METHOD FOR PRODUCING COMPLEX MULTI-CYMATIC STORAGE-STABLE REACTION MIXTURES AND THEIR USE |
DE19832160A1 (en) | 1997-07-31 | 1999-02-04 | Boehringer Mannheim Gmbh | New peptide synthesis bioreactor |
RU2148649C1 (en) | 1998-03-31 | 2000-05-10 | Институт белка РАН | Method and apparatus for preparing polypeptides in acellular system (versions) |
-
1999
- 1999-03-25 RU RU99106754/13A patent/RU2169154C2/en not_active IP Right Cessation
-
2000
- 2000-03-22 CA CA002365237A patent/CA2365237C/en not_active Expired - Lifetime
- 2000-03-22 JP JP2000608772A patent/JP3836679B2/en not_active Expired - Lifetime
- 2000-03-22 WO PCT/EP2000/002508 patent/WO2000058493A1/en active IP Right Grant
- 2000-03-22 DE DE60032227T patent/DE60032227T2/en not_active Expired - Lifetime
- 2000-03-22 EP EP00912629A patent/EP1165826B1/en not_active Expired - Lifetime
- 2000-03-22 AT AT00912629T patent/ATE347610T1/en active
- 2000-03-22 ES ES00912629T patent/ES2277832T3/en not_active Expired - Lifetime
- 2000-03-22 US US09/937,474 patent/US6783957B1/en not_active Expired - Lifetime
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