RU98120809A - METHOD FOR DETERMINING ANTIOXIDATIVE ACTIVITY OF SUBSTANCES - Google Patents

METHOD FOR DETERMINING ANTIOXIDATIVE ACTIVITY OF SUBSTANCES

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Publication number
RU98120809A
RU98120809A RU98120809/14A RU98120809A RU98120809A RU 98120809 A RU98120809 A RU 98120809A RU 98120809/14 A RU98120809/14 A RU 98120809/14A RU 98120809 A RU98120809 A RU 98120809A RU 98120809 A RU98120809 A RU 98120809A
Authority
RU
Russia
Prior art keywords
determining
substances
reaction
antioxidative activity
test substance
Prior art date
Application number
RU98120809/14A
Other languages
Russian (ru)
Other versions
RU2163021C2 (en
Inventor
В.Е. Формазюк
Т.Н. Горшкова
В.И. Сергиенко
Original Assignee
Научно-исследовательский институт физико-химической медицины Министерства здравоохранения РФ
Filing date
Publication date
Application filed by Научно-исследовательский институт физико-химической медицины Министерства здравоохранения РФ filed Critical Научно-исследовательский институт физико-химической медицины Министерства здравоохранения РФ
Priority to RU98120809A priority Critical patent/RU2163021C2/en
Priority claimed from RU98120809A external-priority patent/RU2163021C2/en
Publication of RU98120809A publication Critical patent/RU98120809A/en
Application granted granted Critical
Publication of RU2163021C2 publication Critical patent/RU2163021C2/en

Links

Claims (1)

Способ определения антиокислительной активности вещества путем спектральной оценки их способности подавлять появление ОН-радикалов в реакции Фентон, отличающийся тем, что оценку осуществляют по интенсивности хемилюминесценции реакции Фен-тон по формуле А = (Аo - А+ )/Аo, где Аo - максимальная амплитуда хемилюминесценции реакции Фентон при отсутствии испытуемого вещества, А+ - максимальная амплитуда свечения реакции в присутствии испытуемого вещества и при значениях А>0,3 делают вывод об антиоксидантных свойствах у испытуемого вещества.A method for determining the antioxidant activity of a substance by spectrally evaluating their ability to suppress the appearance of OH radicals in the Fenton reaction, characterized in that the evaluation is carried out according to the intensity of chemiluminescence of the phenon reaction according to the formula A = (A o - A + ) / A o , where A o - the maximum amplitude of the chemiluminescence of the Fenton reaction in the absence of the test substance, A + - the maximum amplitude of the glow of the reaction in the presence of the test substance and at values of A> 0.3, make a conclusion about the antioxidant properties of the test substance.
RU98120809A 1998-11-19 1998-11-19 Method of assay of antioxidant activity of substances RU2163021C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU98120809A RU2163021C2 (en) 1998-11-19 1998-11-19 Method of assay of antioxidant activity of substances

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU98120809A RU2163021C2 (en) 1998-11-19 1998-11-19 Method of assay of antioxidant activity of substances

Publications (2)

Publication Number Publication Date
RU98120809A true RU98120809A (en) 2000-09-10
RU2163021C2 RU2163021C2 (en) 2001-02-10

Family

ID=20212425

Family Applications (1)

Application Number Title Priority Date Filing Date
RU98120809A RU2163021C2 (en) 1998-11-19 1998-11-19 Method of assay of antioxidant activity of substances

Country Status (1)

Country Link
RU (1) RU2163021C2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0621095D0 (en) * 2006-10-23 2006-11-29 Clifmar Associates Ltd Assay method
RU2452947C1 (en) * 2011-01-11 2012-06-10 Государственное образовательное учреждение высшего профессионального образования "Кубанский государственный медицинский университет" (ГОУ ВПО "КГМУ") Method for evaluation of antioxidant-and-energy potential of food substances
RU2516925C1 (en) * 2013-04-04 2014-05-20 Федеральное государственное бюджетное учреждение "Научно-исследовательский институт кардиологии" Сибирского отделения Российской академии медицинских наук Method for estimating effectiveness of antioxidant activity stimulation
RU2571229C2 (en) * 2013-10-31 2015-12-20 федеральное государственное автономное образовательное учреждение высшего профессионального образования "Московский физико-технический институт (государственный университет) Bioluminescent method to detect antioxidant activity of substance
CN117566976B (en) * 2024-01-12 2024-03-26 光大水务（淄博）有限公司 Fenton process accurate dosing control system and method thereof

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