RU98119713A - SUNFLOWER PLANTS (OPTIONS), SUNFLOWER SEEDS WITH HIGH SATURATED FATTY ACIDS, FAT, MARGARINE, SOAP, SHORTENING - Google Patents

SUNFLOWER PLANTS (OPTIONS), SUNFLOWER SEEDS WITH HIGH SATURATED FATTY ACIDS, FAT, MARGARINE, SOAP, SHORTENING

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Publication number
RU98119713A
RU98119713A RU98119713/13A RU98119713A RU98119713A RU 98119713 A RU98119713 A RU 98119713A RU 98119713/13 A RU98119713/13 A RU 98119713/13A RU 98119713 A RU98119713 A RU 98119713A RU 98119713 A RU98119713 A RU 98119713A
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RU
Russia
Prior art keywords
sunflower
seeds
sunflower plant
apb
wild
Prior art date
Application number
RU98119713/13A
Other languages
Russian (ru)
Other versions
RU2185439C2 (en
Inventor
Дэнис Л. Бидни
Лиджиан Ванг
Син Кофлэн
Кристофер Дж. Скелондж
Крейг Хэстингз
Original Assignee
Пайониэ Хай-Брэд Интэнэшнл, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from US08/624,845 external-priority patent/US6084164A/en
Application filed by Пайониэ Хай-Брэд Интэнэшнл, Инк. filed Critical Пайониэ Хай-Брэд Интэнэшнл, Инк.
Publication of RU98119713A publication Critical patent/RU98119713A/en
Application granted granted Critical
Publication of RU2185439C2 publication Critical patent/RU2185439C2/en

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Claims (13)

1. Способ получения растения подсолнечника, включающий инсерцию фрагмента ДНК, отличающийся тем, что проводят инсерцню в подсолнечник дикого типа фрагмента ДНК, транскрибируемая часть которого имеет транскрипт, комплементарный эндогенной мРНК, кодирующей стеароил-АПБ-десатуразу в подсолнечнике дикого типа, затем снижают стеароил-АПБ-десатуразную активность у растения подсолнечника относительно стеароил-АПБ-десатуразной активности у подсолнечника дикого типа и получают трансгенное растение подсолнечника с семенами, содержащими стеарат в количестве 10 - 75% от общего содержания в них жирных кислот.1. A method of obtaining a sunflower plant, including the insertion of a DNA fragment, characterized in that the insertion is performed into the wild-type sunflower DNA fragment, the transcribed part of which has a transcript complementary to endogenous mRNA encoding stearoyl-APB desaturase in wild-type sunflower, then reduce the stearo- APB-desaturase activity in a sunflower plant relative to stearoyl-APB-desaturase activity in a wild-type sunflower and a transgenic sunflower plant with seeds containing stea rat in the amount of 10 - 75% of the total content of fatty acids in them. 2. Способ по п. 1, отличающийся тем, что снижают стеароил-АПБ-десатуразную активность посредством антисмысловой экспрессии указанного фрагмента ДНК. 2. The method according to p. 1, characterized in that they reduce stearoyl-APB-desaturase activity by antisense expression of the indicated DNA fragment. 3. Способ по п. 1, отличающийся тем, что далее проводят скрещивание гомозигот и получают сорт растения подсолнечника с пониженной стеароил-АПБ-десатуразной активностью. 3. The method according to p. 1, characterized in that the homozygotes are crossed and a sunflower plant variety with reduced stearoyl-APB-desaturase activity is obtained. 4. Способ по п. 3, отличающийся тем, что указанный сорт растения подсолнечника дает семена, содержащие менее 10% олеата. 4. The method according to p. 3, characterized in that the said variety of sunflower plants produces seeds containing less than 10% oleate. 5. Способ по п. 1, отличающийся тем, что растение подсолнечника имеет линию (ряд поколений), которая включает сорт подсолнечника с высоким содержанием пальмитата и высоким содержанием олеата в семенах, при этом содержание стеарата составляет 30 - 65% от общего содержания в них жирных кислот. 5. The method according to p. 1, characterized in that the sunflower plant has a line (several generations), which includes a variety of sunflower with a high content of palmitate and a high oleate content in the seeds, while the content of stearate is 30 - 65% of the total content in them fatty acids. 6. Способ по п. 1, отличающийся тем, что трансгенное растение подсолнечника дает семена, содержащие пальмитат в количестве 7 - 10% от общего содержания в них жирных кислот. 6. The method according to p. 1, characterized in that the transgenic sunflower plant produces seeds containing palmitate in an amount of 7-10% of the total content of fatty acids in them. 7. Способ по п. 1, отличающийся тем, что указанный фрагмент ДНК включает плазмиду р7874. 7. The method according to p. 1, characterized in that said DNA fragment comprises plasmid p7874. 8. Способ получения семян подсолнечника, отличающийся тем, что получают трансгенное растение подсолнечника путем инсерции в подсолнечник дикого типа фрагмента ДНК, транскрибируемая часть которого имеет транскрипт, комплементарный эндогенной мРНК, кодирующей стеароил-АПБ-десатуразу в подсолнечнике дикого типа, затем снижают стеароил-АПБ-десатуразную активность у растения подсолнечника относительно стеароил-АПБ-десатуразной активности у подсолнечника дикого типа и добывают из полученного трансгенного растения подсолнечника семена, в которых стеарат составляет 10 - 40% от общего содержания в них жирных кислот. 8. A method of producing sunflower seeds, characterized in that a transgenic sunflower plant is obtained by insertion into the sunflower of a wild-type DNA fragment, the transcribed part of which has a transcript complementary to endogenous mRNA encoding stearoyl-APB desaturase in wild-type sunflower, and then reduce the Stearoil - desaturase activity in a sunflower plant relative to stearoyl-APB desaturase activity in wild-type sunflower and seeds are obtained from the obtained transgenic sunflower plant, stearate which is 10 - 40% of the total content of fatty acids. 9. Рекомбинантная плазмида р7874, предназначенная для получения растения подсолнечника. 9. Recombinant plasmid p7874, designed to obtain a sunflower plant. 10. Жир для глазури, содержащий жирную кислоту, произведенную из семян растения подсолнечника, полученных согласно способу по любому из пп. 1-8. 10. Fat for glaze containing a fatty acid produced from seeds of a sunflower plant obtained according to the method according to any one of claims. 1-8. 11. Маргарин, содержащий жирную кислоту, произведенную из семян растения подсолнечника, полученных согласно способу по любому из пп. 1-8. 11. Margarine containing fatty acid produced from seeds of a sunflower plant, obtained according to the method according to any one of paragraphs. 1-8. 12. Мыло, содержащее жирную кислоту, произведенную из семян растения подсолнечника, полученных согласно способу по любому из пп. 1-8. 12. Soap containing a fatty acid produced from seeds of a sunflower plant obtained according to the method according to any one of paragraphs. 1-8. 13. Шортенинг (жир в тесто), содержащий жирную кислоту, произведенную из семян растения подсолнечника, полученных согласно способу по любому из пп. 1-8. 13. Shortening (fat in the dough) containing a fatty acid produced from seeds of a sunflower plant obtained according to the method according to any one of claims. 1-8.
RU98119713/13A 1996-03-25 1997-02-10 Method of preparing sunflower plant, method of producing sunflower seeds and recombinant plasmid RU2185439C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/624,845 US6084164A (en) 1996-03-25 1996-03-25 Sunflower seeds with enhanced saturated fatty acid contents
US08/624,845 1996-03-25

Publications (2)

Publication Number Publication Date
RU98119713A true RU98119713A (en) 2000-11-20
RU2185439C2 RU2185439C2 (en) 2002-07-20

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RU98119713/13A RU2185439C2 (en) 1996-03-25 1997-02-10 Method of preparing sunflower plant, method of producing sunflower seeds and recombinant plasmid

Country Status (12)

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US (2) US6084164A (en)
EP (1) EP0889962A1 (en)
AR (1) AR006977A1 (en)
AU (1) AU727803C (en)
BG (1) BG102859A (en)
CA (1) CA2250091A1 (en)
HU (1) HUP0103081A3 (en)
RO (1) RO117993B1 (en)
RU (1) RU2185439C2 (en)
SK (1) SK132098A3 (en)
TR (1) TR199801897T2 (en)
WO (1) WO1997035987A1 (en)

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