RU98117042A - ENVIRONMENT FOR PRESERVATION OF LIVER CELLS - Google Patents
ENVIRONMENT FOR PRESERVATION OF LIVER CELLSInfo
- Publication number
- RU98117042A RU98117042A RU98117042/13A RU98117042A RU98117042A RU 98117042 A RU98117042 A RU 98117042A RU 98117042/13 A RU98117042/13 A RU 98117042/13A RU 98117042 A RU98117042 A RU 98117042A RU 98117042 A RU98117042 A RU 98117042A
- Authority
- RU
- Russia
- Prior art keywords
- environment
- preservation
- liver cells
- solution
- polyvinylpyrrolidone
- Prior art date
Links
- 210000004185 Liver Anatomy 0.000 title claims 2
- 238000004321 preservation Methods 0.000 title claims 2
- 229940069337 Potassium Orotate Drugs 0.000 claims 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 claims 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims 2
- DHBUISJCVRMTAZ-UHFFFAOYSA-M potassium;2,4-dioxo-1H-pyrimidine-6-carboxylate Chemical compound [K+].[O-]C(=O)C1=CC(=O)NC(=O)N1 DHBUISJCVRMTAZ-UHFFFAOYSA-M 0.000 claims 2
- 102100001249 ALB Human genes 0.000 claims 1
- 101710027066 ALB Proteins 0.000 claims 1
- 102000008100 Human Serum Albumin Human genes 0.000 claims 1
- 108091006822 Human Serum Albumin Proteins 0.000 claims 1
- 229940050528 albumin Drugs 0.000 claims 1
- 239000002609 media Substances 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
Claims (1)
Среда RPMI - 10,0-10,5
Поливинилпирролидон - 1,0-1,5
1% раствор альбумина человеческого - 3,0-3,6
Калий оротат - 1,0-1,2
рН 7,6The environment for the preservation of liver cells, containing as a cryoprotector low molecular weight polyvinylpyrrolidone, characterized in that as life-supporting components additionally contains nutrient medium RPMI, 1% solution of albumin, potassium orotate in the following ratio, wt.h .:
RPMI environment - 10.0-10.5
Polyvinylpyrrolidone - 1.0-1.5
1% solution of human albumin - 3.0-3.6
Potassium Orotate - 1.0-1.2
pH 7.6
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU98117042A RU2161198C2 (en) | 1998-09-07 | 1998-09-07 | Medium for liver cells conservation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU98117042A RU2161198C2 (en) | 1998-09-07 | 1998-09-07 | Medium for liver cells conservation |
Publications (2)
Publication Number | Publication Date |
---|---|
RU98117042A true RU98117042A (en) | 2000-08-27 |
RU2161198C2 RU2161198C2 (en) | 2000-12-27 |
Family
ID=20210407
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU98117042A RU2161198C2 (en) | 1998-09-07 | 1998-09-07 | Medium for liver cells conservation |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2161198C2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110463689B (en) * | 2019-07-31 | 2022-12-30 | 周明 | Primary hepatocyte cryopreservation liquid, hepatocyte cryopreservation method and hepatocyte recovery method |
-
1998
- 1998-09-07 RU RU98117042A patent/RU2161198C2/en active
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