RU98108026A - OBTAINING L (+) - LACTATE - Google Patents

OBTAINING L (+) - LACTATE

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Publication number
RU98108026A
RU98108026A RU98108026/13A RU98108026A RU98108026A RU 98108026 A RU98108026 A RU 98108026A RU 98108026/13 A RU98108026/13 A RU 98108026/13A RU 98108026 A RU98108026 A RU 98108026A RU 98108026 A RU98108026 A RU 98108026A
Authority
RU
Russia
Prior art keywords
strain
vector
sequence
conjugative
modified sequence
Prior art date
Application number
RU98108026/13A
Other languages
Russian (ru)
Other versions
RU2202610C2 (en
Inventor
Лапьер Люсиан
Молле Бит
Деллей Мишель
Эдуар Жермон Жак
Рэймонд Придмор Дэвид
Original Assignee
Сосьете Де Продюи Нестле С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP97201337A external-priority patent/EP0875579B1/en
Application filed by Сосьете Де Продюи Нестле С.А. filed Critical Сосьете Де Продюи Нестле С.А.
Publication of RU98108026A publication Critical patent/RU98108026A/en
Application granted granted Critical
Publication of RU2202610C2 publication Critical patent/RU2202610C2/en

Links

Claims (9)

1. Бактериальный штамм со способностью выживать в кишечнике, связываться с клетками кишечника человека и осуществлять иммуномодуляцию, отличающийся тем, что он генетически перестроен так, что продуцирует только L(+)-лактат.1. A bacterial strain with the ability to survive in the intestine, bind to human intestinal cells and carry out immunomodulation, characterized in that it is genetically rearranged so that it produces only L (+) - lactate. 2. Штамм по п. 1, причем ген, кодирующий фермент D-лактатдегидрогеназу, инактивируется. 2. The strain according to claim 1, wherein the gene encoding the enzyme D-lactate dehydrogenase is inactivated. 3. Штамм по п. 1, отличающийся тем, что он является штаммом Lactobacilius acidophilus, Lactobacillus johnsonii, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus amylovorus или Lactobacillus gallinarum. 3. The strain according to claim 1, characterized in that it is a strain of Lactobacilius acidophilus, Lactobacillus johnsonii, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus amylovorus or Lactobacillus gallinarum. 4. Штамм по п. 1, помещенный на хранение в CNCM под номером 1-1851. 4. The strain according to claim 1, placed in storage at CNCM under the number 1-1851. 5. Штамм по п. 1, помещенный на хранение в CNCM под номером 1-1852. 5. The strain according to claim 1, placed in storage at CNCM under the number 1-1852. 6. Способ получения штамма по п. 1, при котором: последовательность гена, кодирующего D-лактатдегидрогеназу, выделяют из бактериального штамма-хозяина со способностью выживать в кишечнике, связываться с клетками кишечника человека и осуществлять иммуномодуляцию, осуществляется направленный мутагенез у этой последовательности с получением модифицированной последовательности, эту модифицированную последовательность интегрируют в конъюгативный вектор, конъюгативный вектор переносится с помощью конъюгации в бактериальный штамм-хозяин, и затем производится отбор бактерий-хозяев, у которых последовательность, кодирующая фермент D-лактатдегидрогеназу, была заменена с помощью гомологичной рекомбинации модифицированной последовательностью. 6. The method of producing a strain according to claim 1, wherein: the sequence of a gene encoding D-lactate dehydrogenase is isolated from a bacterial host strain with the ability to survive in the intestine, bind to human intestinal cells and carry out immunomodulation, directed mutagenesis is performed in this sequence to obtain modified sequence, this modified sequence is integrated into the conjugative vector, the conjugative vector is transferred by conjugation to the bacterial host strain, and then selection is made of host bacteria in which the sequence encoding the enzyme D-lactate dehydrogenase has been replaced by homologous recombination with a modified sequence. 7. Способ по п. 6, причем чтобы интегрировать модифицированную последовательность в конъюгативный вектор выбирают донорский бактериальный штамм, содержащий конъюгативный вектор, который не обладает способностью реплицироваться в бактериальном штамме-хозяине, конструкцию получают путем лигирования модифицированной последовательности в первый вектор, который неспособен размножаться в донорском штамме, эту конструкцию вводят в донорский бактериальный штамм, и затем отбирают донорские бактерии, в которых первый вектор и конъюгативный вектор прошли рекомбинацию. 7. The method according to claim 6, wherein in order to integrate the modified sequence into the conjugative vector, a donor bacterial strain containing a conjugative vector that does not replicate in the bacterial host strain is selected, the construct is obtained by ligation of the modified sequence into a first vector that is unable to propagate in donor strain, this construct is introduced into the donor bacterial strain, and then donor bacteria are selected in which the first vector and the conjugative The rector passed recombination. 8. Способ по п. 6, причем отбирают бактерии-хозяева, в которых последовательности ДНК конъюгативного вектора были удалены из генома, за исключением модифицированной последовательности. 8. The method of claim 6, wherein host bacteria are selected in which the DNA sequences of the conjugative vector have been removed from the genome, with the exception of the modified sequence. 9. Способ применения бактериального штамма, полученного путем осуществления способа по одному из пп. 6 - 8, в изготовлении пищевых продуктов. 9. A method of using a bacterial strain obtained by implementing the method according to one of claims. 6-8, in the manufacture of food products.
RU98108026/13A 1997-05-03 1998-04-28 Lactobacillus johnsonii as producer of l-(+)-lactate (variants) and method for preparing bacterial strain RU2202610C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97201337A EP0875579B1 (en) 1997-05-03 1997-05-03 Production of L(+)lactate
EP97201337.9 1997-05-03

Publications (2)

Publication Number Publication Date
RU98108026A true RU98108026A (en) 2000-02-20
RU2202610C2 RU2202610C2 (en) 2003-04-20

Family

ID=8228296

Family Applications (1)

Application Number Title Priority Date Filing Date
RU98108026/13A RU2202610C2 (en) 1997-05-03 1998-04-28 Lactobacillus johnsonii as producer of l-(+)-lactate (variants) and method for preparing bacterial strain

Country Status (16)

Country Link
US (2) US6110725A (en)
EP (1) EP0875579B1 (en)
JP (1) JPH10313884A (en)
KR (1) KR100503707B1 (en)
AR (1) AR015376A1 (en)
AT (1) ATE365805T1 (en)
AU (1) AU753558B2 (en)
CA (1) CA2230435C (en)
DE (1) DE69737853T2 (en)
DK (1) DK0875579T3 (en)
ID (1) ID20238A (en)
MY (1) MY131830A (en)
NO (1) NO324916B1 (en)
NZ (1) NZ330351A (en)
RU (1) RU2202610C2 (en)
ZA (1) ZA983697B (en)

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RU2461619C1 (en) * 2011-09-12 2012-09-20 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Горский государственный аграрный университет" Lactobacillus gallinarum strain used for preparing fermented milk products

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DK0875579T3 (en) * 1997-05-03 2007-10-22 Nestle Sa Preparation of L (+) lactate
WO2003049525A2 (en) * 2001-11-23 2003-06-19 Cargill Dow Llc Methods and materials for the production of organic products in cells of $i(candida) species
JP2006506042A (en) * 2002-04-09 2006-02-23 ソシエテ デ プロデユイ ネツスル ソシエテ アノニム Genome of La1-Lactobacillus strain
WO2003102200A2 (en) * 2002-05-30 2003-12-11 Cargill Dow Llc Fermentation process using specific oxygen uptake rates as a process control
KR100449915B1 (en) * 2002-10-30 2004-09-22 주식회사한국야쿠르트 Lactobacillus acidophilus ky1909
JP2012531190A (en) * 2009-06-30 2012-12-10 セーホーエル．ハンセン アクティーゼルスカブ Method for producing fermented dairy products
GB0914878D0 (en) 2009-08-26 2009-09-30 Plant Bioscience Ltd Competetive exclusion compositions and methods
WO2012130965A1 (en) * 2011-03-29 2012-10-04 Nestec S.A. Natural derivative of a well known and successful probiotic strain deficient in d-lactic acid production
SG193566A1 (en) * 2011-03-29 2013-10-30 Nestec Sa Natural derivative of the lactobacillus johnsonii strain cncm i-1225 deficient in d-lactic acid production
US9309492B2 (en) * 2011-03-29 2016-04-12 Nestec S.A. Derivative of the Lactobacilus johnsonii strain CNCM I-1225, deficient in D-lactic acid production and with an improved shelf life
BR112013024831A2 (en) * 2011-03-29 2016-12-20 Nestec Sa natural derivative of the cncm i-1225 strain of lactobacillus johnsonii, deficient in d = lactic acid production and with a higher immune profile
KR101295369B1 (en) * 2011-10-14 2013-08-09 한국화학연구원 L―lactic acid producing genetically modified microorganisms and the method for preparing L―lactic acid using the same
KR101438882B1 (en) 2012-04-24 2014-09-05 씨제이제일제당 (주) Novel microorganism for producing D-type lactic acid

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CH683223A5 (en) * 1991-10-25 1994-02-15 Nestle Sa A process for preparing an acidified milk.
EP0768375B2 (en) * 1992-07-06 2002-06-12 Societe Des Produits Nestle S.A. Lactic bacteria
US5747310A (en) * 1992-07-10 1998-05-05 Meiji Milk Products Co., Ltd. Gene integration into chromosomes of lactobacillus delbrueckii species and integrants thereof
US5416020A (en) * 1992-09-29 1995-05-16 Bio-Technical Resources Lactobacillus delbrueckii ssp. bulgaricus strain and fermentation process for producing L-(+)-lactic acid
PH31093A (en) * 1993-08-06 1998-02-05 Nestec Ltd Lactobacillus bulgaricus having decreased acid production and/or improved aroma and flavor production and food composition comprising said lactobacillus.
EP0638642A1 (en) * 1993-08-06 1995-02-15 Societe Des Produits Nestle S.A. Lactobacillus bulgaricus having decreased acid production and/or improved aroma and flavor production, and food composition comprising said lactobacillus bulgaricus
DK0875579T3 (en) * 1997-05-03 2007-10-22 Nestle Sa Preparation of L (+) lactate

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2461619C1 (en) * 2011-09-12 2012-09-20 Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Горский государственный аграрный университет" Lactobacillus gallinarum strain used for preparing fermented milk products

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